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公开(公告)号：US20170313999A1
公开(公告)日：2017-11-02
申请号：US15602622
申请日：2017-05-23
发明人： Greg Pogue , Ernie Hiatt , Romy Kandzia , Stefan Werner , Frank Thieme , Tsafrir Mor , Steven Hume
CPC分类号： C12N9/18 , A61K38/465 , C12Y301/01008
摘要： A new, reliable, easily scalable and reproducible method for the production of recombinant butyrylcholinesterase (rBuChE) is provided. Through the utilization of a plant transfection procedure, various plant strains have been shown to generate effective and scalable amounts of rBuChE under acceptable manufacturing processes to permit reliable levels of such enzymes for desired nerve agent protection requirements (including tetrameric products). As well, such methods in engineered plant lines have shown suitable production of these enzymes in tetramer form with glycan formation and sialylation (for terminal groups) to allow for optimal potency against organophosphorus agent exposure as well as proper immunogenic response within the plant sources. The overall production method, including the transfection and production within mammalian cells, as well as the process steps involved for such a reliable sourcing platform from plants is thus encompassed within the invention.
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公开(公告)号：US10035994B2
公开(公告)日：2018-07-31
申请号：US15602622
申请日：2017-05-23
发明人： Greg Pogue , Ernie Hiatt , Romy Kandzia , Stefan Werner , Frank Thieme , Tsafrir Mor , Steven Hume
CPC分类号： C12N9/18 , A61K38/465 , C12Y301/01008
摘要： A new, reliable, easily scalable and reproducible method for the production of recombinant butyrylcholinesterase (rBuChE) is provided. Through the utilization of a plant transfection procedure, various plant strains have been shown to generate effective and scalable amounts of rBuChE under acceptable manufacturing processes to permit reliable levels of such enzymes for desired nerve agent protection requirements (including tetrameric products). As well, such methods in engineered plant lines have shown suitable production of these enzymes in tetramer form with glycan formation and sialylation (for terminal groups) to allow for optimal potency against organophosphorus agent exposure as well as proper immunogenic response within the plant sources. The overall production method, including the transfection and production within mammalian cells, as well as the process steps involved for such a reliable sourcing platform from plants is thus encompassed within the invention.
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公开(公告)号：US09688970B2
公开(公告)日：2017-06-27
申请号：US14448545
申请日：2014-07-31
发明人： Greg Pogue , Ernie Hiatt , Romy Kandzia , Stefan Werner , Frank Thieme , Tsafrir Mor , Steven Hume
CPC分类号： C12N9/18 , A61K38/465 , C12Y301/01008
摘要： A new, reliable, easily scalable and reproducible method for the production of recombinant butyrylcholinesterase (rBuChE) is provided. Through the utilization of a plant transfection procedure, various plant strains have been shown to generate effective and scalable amounts of rBuChE under acceptable manufacturing processes to permit reliable levels of such enzymes for desired nerve agent protection requirements (including tetrameric products). As well, such methods in engineered plant lines have shown suitable production of these enzymes in tetramer form with glycan formation and sialyalation (for terminal groups) to allow for optimal potency against organophosphorus agent exposure as well as proper immunogenic response within the plant sources. The overall production method, including the transfection and production within mammalian cells, as well as the process steps involved for such a reliable sourcing platform from plants is thus encompassed within the invention.
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公开(公告)号：US20060013831A1
公开(公告)日：2006-01-19
申请号：US10506796
申请日：2003-03-06
申请人： Tsafrir Mor , Nobuyuki Matoba , Charles Arntzen
发明人： Tsafrir Mor , Nobuyuki Matoba , Charles Arntzen
CPC分类号： C07K14/005 , A61K39/39 , A61K2039/541 , A61K2039/55516 , A61K2039/6037 , A61K2039/6075 , C12N2740/16122
摘要： A composition and method for enhancing immune response in a living organism is disclosed. In particular, the present disclosure provides an adjuvant peptide for use in raising an immune response to an antigen. The adjuvant peptide is selected from a group of peptides with an HIV-related sequence. Additionally, the adjuvant peptide can comprise a fusion-protein that acts as a mucosal adjuvant. The adjuvant peptide can be transformed into one or more living cells, such that the mucosal adjuvant can be produced in living cells and then administered by systemic, mucosal or epidermal delivery.
摘要翻译： 公开了一种增强活体内免疫应答的组合物和方法。 特别地，本公开提供了用于提高对抗原的免疫应答的佐剂肽。 佐剂肽选自具有HIV相关序列的肽组。 另外，佐剂肽可以包含充当粘膜佐剂的融合蛋白。 辅助肽可以转化成一个或多个活细胞，使得粘膜佐剂可以在活细胞中产生，然后通过全身，粘膜或表皮递送进行施用。
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5.发明申请MATERIALS AND METHODS FOR TREATING OR PREVENTING ORGANOPHOSPHATE EXPOSURE ASSOCIATED DAMAGE 审中-公开用于治疗或预防有机磷酸盐暴露相关损害的材料和方法公开(公告)号：US20110064716A1
公开(公告)日：2011-03-17
申请号：US12673984
申请日：2007-08-20
申请人： Hermona Soreq , Tsafrir Mor
发明人： Hermona Soreq , Tsafrir Mor
CPC分类号： A61K9/0019 , A61K9/0073 , A61K9/7007 , A61K38/465 , C12Y301/01007
摘要： A method of treating or preventing organophosphate exposure associated damage in a subject in need thereof is provided. The method comprising providing the subject with a therapeutically effective amount of AChE-R to thereby treat the organophosphate exposure associated damage in the subject.
摘要翻译： 提供了在有需要的受试者中治疗或预防有机磷酸盐暴露相关损伤的方法。 所述方法包括向受试者提供治疗有效量的AChE-R，从而治疗受试者中与有机磷酸盐暴露相关的损伤。
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公开(公告)号：US20150184138A1
公开(公告)日：2015-07-02
申请号：US14448545
申请日：2014-07-31
申请人： Greg Pogue , Ernie Hiatt , Romy Kandzia , Stefan Werner , Frank Thieme , Tsafrir Mor
发明人： Greg Pogue , Ernie Hiatt , Romy Kandzia , Stefan Werner , Frank Thieme , Tsafrir Mor
CPC分类号： C12N9/18 , A61K38/465 , C12Y301/01008
摘要： A new, reliable, easily scalable and reproducible method for the production of recombinant butyrylcholinesterase (rBuChE) is provided. Through the utilization of a plant transfection procedure, various plant strains have been shown to generate effective and scalable amounts of rBuChE under acceptable manufacturing processes to permit reliable levels of such enzymes for desired nerve agent protection requirements (including tetrameric products). As well, such methods in engineered plant lines have shown suitable production of these enzymes in tetramer form with glycan formation and sialyalation (for terminal groups) to allow for optimal potency against organophosphorus agent exposure as well as proper immunogenic response within the plant sources. The overall production method, including the transfection and production within mammalian cells, as well as the process steps involved for such a reliable sourcing platform from plants is thus encompassed within the invention.
摘要翻译： 提供了一种用于生产重组丁酰胆碱酯酶（rBuChE）的新的，可靠的，易于扩展和重现的方法。 通过利用植物转染方法，已经显示各种植物菌株在可接受的制造过程中产生有效和可缩放量的rBuChE，以允许可靠水平的这些酶用于所需的神经药物保护要求（包括四聚物产品）。 同样，工程化植物品系中的这些方法已经显示出以四聚体形式合适地生产这些酶，其中聚糖形成和sialyalation（用于末端基团）以允许针对有机磷剂暴露的最佳效力以及在植物来源内的适当的免疫原性反应。 因此，包括哺乳动物细胞内的转染和生产的总体生产方法以及从植物获得这种可靠的采购平台所涉及的工艺步骤。
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7.发明申请COMPOSITIONS AND METHODS FOR THE PRODUCTION AND USE OF HUMAN CHOLINESTERASES 审中-公开用于生产和使用人类胆固醇的组合物和方法公开(公告)号：US20140248684A1
公开(公告)日：2014-09-04
申请号：US14344974
申请日：2012-09-17
CPC分类号： C12N9/18 , C12N15/8255 , C12N15/8257 , C12Y301/01008
摘要： In some aspects, the present invention relates to compositions and methods for the production of human cholinesterases. More particularly, it relates to methods for the production of human cholinesterases using transient expression and vectors for producing the same. In one aspect, the present invention relates to a plant viral vector encoding a plant codon-optimized DNA sequence that results in accumulation of the cholinesterase in a plant leaf at levels greater than 20 mg, and in some embodiments greater than 200 mg, of the enzyme per kilogram of the plant leaf.
摘要翻译： 在一些方面，本发明涉及用于生产人胆碱酯酶的组合物和方法。 更具体地说，本发明涉及使用瞬时表达生产人胆碱酯酶的方法和用于生产人胆碱酯酶的方法。 一方面，本发明涉及编码植物密码子优化的DNA序列的植物病毒载体，其导致胆碱酯酶在植物叶中的积累，其水平大于20mg，在一些实施方案中大于200mg， 酶每千克植物叶。
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