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公开(公告)号:US12116627B2
公开(公告)日:2024-10-15
申请号:US17034024
申请日:2020-09-28
申请人: Tsinghua University
发明人: Ting Zhu , Xianyu Liu , Wenjun Jiang , Min Wang
IPC分类号: C12Q1/6869 , C12N15/10 , C12Q1/6806
CPC分类号: C12Q1/6869 , C12N15/1096 , C12Q1/6806
摘要: Methods of sequencing and producing nucleic acid sequences are provided. Accordingly there are provided methods of sequencing a nucleic acid sequence comprising L-nucleotides comprising subjecting the nucleic acid sequence comprising the L-nucleotides to a chemical sequencing method. Also provided is a method of reverse transcribing a ribose nucleic acid sequence into a deoxyribose nucleic acid sequence comprising catalyzing reverse transcription of the ribose nucleic acid sequence with a Sulfolobus solfataricus P2 DNA polymerase IV (Dpo4).