公开(公告)号：US20100184200A1

公开(公告)日：2010-07-22

申请号：US12461045

申请日：2009-07-30

申请人： Tsong-Rong Yan ,  Hung-Che Chou ,  Chao-Fa Lee

发明人： Tsong-Rong Yan ,  Hung-Che Chou ,  Chao-Fa Lee

IPC分类号： C12M1/34

CPC分类号： C12M41/36 ,  C12M23/08 ,  C12M23/46

摘要： The present invention relates to a cell culture device which includes: a cell culture container comprising a container body and a neck connecting to the container body wherein the container contains a top wall, a bottom wall having a first opening, and at least one side wall to define a cell culture zone, and an end of the neck has a second opening for cells or culture media being injected into the cell culture zone; a biosensor chip having a detective surface which faces the top wall of the cell culture container; and a fastening component for the biosensor chip being fastened in the first opening of the bottom wall. The cell culture of the present invention can be used for real-time detection of the cell growth and further for drug screening.

摘要翻译： 细胞培养装置技术领域本发明涉及一种细胞培养装置，其特征在于，包括：细胞培养容器，其包括容器主体和与所述容器主体连接的颈部，所述容器包含顶壁，具有第一开口的底壁和至少一个侧壁 以限定细胞培养区，并且颈部的末端具有注入细胞培养区的细胞或培养基的第二个开口; 具有面向细胞培养容器的顶壁的检测面的生物传感器芯片; 并且用于生物传感器芯片的紧固部件紧固在底壁的第一开口中。 本发明的细胞培养物可用于实时检测细胞生长并进一步用于药物筛选。

公开(公告)号：US07509860B2

公开(公告)日：2009-03-31

申请号：US11783943

申请日：2007-04-13

申请人： Chao-Fa Lee ,  Tsong-Rong Yan ,  Cheng-Hsing Kuo

发明人： Chao-Fa Lee ,  Tsong-Rong Yan ,  Cheng-Hsing Kuo

IPC分类号： G01H13/00 ,  G01N29/02

CPC分类号： G01H13/00

摘要： This present invention relates to a system for measuring resonant frequency and delay time of the quartz crystal microbalance. The system includes a transistor oscillating circuit, a switch circuit, a comparator circuit, and a control circuit. The transistor oscillating circuit comprises a quartz oscillator for generating an original oscillating signal. The switch circuit is coupled to the transistor oscillating circuit for outputting a start signal to generate the original oscillating signal. The comparator circuit is coupled to the transistor oscillating circuit for transforming the original oscillating signal to a square wave oscillating signal. The control circuit is coupled to the comparator circuit for receiving the square oscillating signal to estimate the resonant frequency and the delay time of the quartz crystal microbalance.

摘要翻译： 本发明涉及一种用于测量石英晶体微量天平的谐振频率和延迟时间的系统。 该系统包括晶体管振荡电路，开关电路，比较器电路和控制电路。 晶体管振荡电路包括用于产生原始振荡信号的石英振荡器。 开关电路耦合到晶体管振荡电路，用于输出起始信号以产生原始振荡信号。 比较器电路耦合到晶体管振荡电路，用于将原始振荡信号变换成方波振荡信号。 控制电路耦合到比较器电路，用于接收平方振荡信号以估计石英晶体微量天平的谐振频率和延迟时间。

公开(公告)号：US20120077257A1

公开(公告)日：2012-03-29

申请号：US12929543

申请日：2011-02-01

申请人： Chao-Fa Lee ,  Tsong-Rong Yan ,  Cheng-Hsing Kuo

发明人： Chao-Fa Lee ,  Tsong-Rong Yan ,  Cheng-Hsing Kuo

IPC分类号： C12M1/42

CPC分类号： C12M41/46 ,  C12M41/36

摘要： A cell measurement system measures changes of frequency and transepithelial electrical resistance of a tested cell sample. The cell measurement system includes a quartz crystal sensing module, an oscillation module, a periodic wave-generation module, a low-pass filtration module, and a control module. The cell measurement system of the present invention can simultaneously measure changes of frequency and transepithelial electrical resistance of a tested cell sample during cell growth so that the growth level and healthy condition of the cells and degree of a monolayer completion of the cells can be determined.

摘要翻译： 细胞测量系统测量测试细胞样品的频率和跨上皮电阻的变化。 电池测量系统包括石英晶体感测模块，振荡模块，周期波发生模块，低通滤波模块和控制模块。 本发明的细胞测量系统可以同时测量细胞生长期间测试细胞样品的频率和跨上皮电阻的变化，从而可以确定细胞的生长水平和健康状况以及细胞单层完成程度。

公开(公告)号：US20080184801A1

公开(公告)日：2008-08-07

申请号：US11783943

申请日：2007-04-13

申请人： Chao-Fa Lee ,  Tsong-Rong Yan ,  Cheng-Hsing Kuo

发明人： Chao-Fa Lee ,  Tsong-Rong Yan ,  Cheng-Hsing Kuo

IPC分类号： G01H13/00 ,  G01H3/06

CPC分类号： G01H13/00

摘要： This present invention relates to a system for measuring resonant frequency and delay time of the quartz crystal microbalance. The system includes a transistor oscillating circuit, a switch circuit, a comparator circuit, and a control circuit. The transistor oscillating circuit comprises a quartz oscillator for generating an original oscillating signal. The switch circuit is coupled to the transistor oscillating circuit for outputting a start signal to generate the original oscillating signal. The comparator circuit is coupled to the transistor oscillating circuit for transforming the original oscillating signal to a square wave oscillating signal. The control circuit is coupled to the comparator circuit for receiving the square oscillating signal to estimate the resonant frequency and the delay time of the quartz crystal microbalance.

摘要翻译： 本发明涉及一种用于测量石英晶体微量天平的谐振频率和延迟时间的系统。 该系统包括晶体管振荡电路，开关电路，比较器电路和控制电路。 晶体管振荡电路包括用于产生原始振荡信号的石英振荡器。 开关电路耦合到晶体管振荡电路，用于输出起始信号以产生原始振荡信号。 比较器电路耦合到晶体管振荡电路，用于将原始振荡信号变换成方波振荡信号。 控制电路耦合到比较器电路，用于接收平方振荡信号以估计石英晶体微量天平的谐振频率和延迟时间。

公开(公告)号：US07814795B2

公开(公告)日：2010-10-19

申请号：US12107788

申请日：2008-04-23

申请人： Chao-Fa Lee ,  Cheng-Hsing Kuo ,  Tsong-Rong Yan

发明人： Chao-Fa Lee ,  Cheng-Hsing Kuo ,  Tsong-Rong Yan

IPC分类号： G01G11/04

CPC分类号： G01G3/13 ,  G01G3/16

摘要： A dual mode measurement system with quartz crystal microbalance (QCM) is provided, which includes a quartz sensing component, a first measurement circuit, a second measurement circuit and a switch unit. When the first measurement circuit is selected through the switch unit, the first measurement circuit and the quartz sensing component form an oscillation circuit and output a resonance signal. When the second measurement circuit is selected through the switch unit, the second measurement circuit outputs a frequency scanning signal to scan the quartz sensing component, so as to output an impedance sensing signal.

摘要翻译： 提供了具有石英晶体微量天平（QCM）的双模测量系统，其包括石英感测元件，第一测量电路，第二测量电路和开关单元。 当通过开关单元选择第一测量电路时，第一测量电路和石英感测组件形成振荡电路并输出谐振信号。 当通过开关单元选择第二测量电路时，第二测量电路输出频率扫描信号以扫描石英感测部件，以便输出阻抗感测信号。

公开(公告)号：US20090165560A1

公开(公告)日：2009-07-02

申请号：US12107788

申请日：2008-04-23

申请人： Chao-Fa Lee ,  Cheng-Hsing Kuo ,  Tsong-Rong Yan

发明人： Chao-Fa Lee ,  Cheng-Hsing Kuo ,  Tsong-Rong Yan

IPC分类号： G01G11/04

CPC分类号： G01G3/13 ,  G01G3/16

摘要： A dual mode measurement system with quartz crystal microbalance (QCM) is provided, which includes a quartz sensing component, a first measurement circuit, a second measurement circuit and a switch unit. When the first measurement circuit is selected through the switch unit, the first measurement circuit and the quartz sensing component form an oscillation circuit and output a resonance signal. When the second measurement circuit is selected through the switch unit, the second measurement circuit outputs a frequency scanning signal to scan the quartz sensing component, so as to output an impedance sensing signal.

摘要翻译： 提供了具有石英晶体微量天平（QCM）的双模测量系统，其包括石英感测元件，第一测量电路，第二测量电路和开关单元。 当通过开关单元选择第一测量电路时，第一测量电路和石英感测组件形成振荡电路并输出谐振信号。 当通过开关单元选择第二测量电路时，第二测量电路输出频率扫描信号以扫描石英感测部件，以便输出阻抗感测信号。