公开(公告)号：US11732294B2

公开(公告)日：2023-08-22

申请号：US16590301

申请日：2019-10-01

Applicant: Twist Bioscience Corporation

Inventor： Ramsey Ibrahim Zeitoun ,  Siyuan Chen ,  Richard Gantt ,  Kristin D. Butcher ,  E. Hutson Chilton

IPC: C12Q1/68 ,  C12Q1/6869 ,  G16B40/10 ,  C40B30/10 ,  C40B70/00

CPC classification number: C12Q1/6869 ,  C40B30/10 ,  C40B70/00 ,  G16B40/10

Abstract: Provided herein are compositions, methods and systems relating to libraries of polynucleotides such that the libraries allow for accurate and efficient hybridization after binding to target sequences. Further provided herein are probes, blockers, additives, buffers, and methods that result in improved hybridization. Such compositions and methods are useful for improvement of Next Generation Sequencing applications, such as reducing off-target binding or reducing workflow times.

公开(公告)号：US11492665B2

公开(公告)日：2022-11-08

申请号：US16417023

申请日：2019-05-20

Applicant: Twist Bioscience Corporation

Inventor： Ramsey Ibrahim Zeitoun ,  Siyuan Chen ,  Richard Gantt ,  Kristin D. Butcher ,  E. Hutson Chilton

IPC: C12Q1/68 ,  C12Q1/6869 ,  G16B40/10 ,  C40B30/10 ,  C40B70/00

Abstract: Provided herein are compositions, methods and systems relating to libraries of polynucleotides such that the libraries allow for accurate and efficient hybridization after binding to target sequences. Further provided herein are probes, blockers, additives, buffers, and methods that result in improved hybridization. Such compositions and methods are useful for improvement of Next Generation Sequencing applications, such as reducing off-target binding or reducing workflow times.