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公开(公告)号：US11384136B2
公开(公告)日：2022-07-12
申请号：US16674003
申请日：2019-11-05
Applicant: UCB BIOPHARMA SPRL
Inventor： Jean-Pascal Pierre Bilgischer , Philip Jonathan Bassett , Mark Robert Pearce-Higgins , Andrew John Kenny
Abstract: The present disclosure relates to a method for the manufacture of recombinant antibody molecules comprising culturing a host cell sample transformed with an expression vector encoding a recombinant antibody molecule; adding an extraction buffer to the sample; and subjecting the sample to a heat treatment step; wherein the pH of the sample is detected after addition of the extraction buffer, and optionally adjusted, to ensure that the pH of the sample is 6 to 9 prior to the heat treatment step.
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