公开(公告)号：US11702459B2

公开(公告)日：2023-07-18

申请号：US16563987

申请日：2019-09-09

Applicant: UNIVERSITÄT BASEL

Inventor： Gennaro De Libero ,  Marco Lepore ,  Lucia Mori

IPC: C07K14/725 ,  C12N5/0783 ,  G01N1/30 ,  G01N33/569 ,  C12N5/09 ,  C12N15/85 ,  A61K35/17 ,  A61P35/00

CPC classification number: C07K14/7051 ,  A61K35/17 ,  A61P35/00 ,  C12N5/0636 ,  C12N5/0693 ,  C12N15/85 ,  G01N1/30 ,  G01N33/56966 ,  C12N2501/2302 ,  C12N2501/2307 ,  C12N2501/2315 ,  C12N2502/1114 ,  C12N2502/30

Abstract: The invention relates to a method of isolating a T cell that expresses a T cell receptor capable of binding specifically to an antigen presented by a cancer cell in association with an MR1 molecule. The method comprises the steps of (a) providing a preparation of T cells, (b) contacting the preparation with cancer cells expressing MR1 protein; (c) isolating a T cell that is specifically reactive to said cancer cells.
The invention further relates to a method of preparing a T cell preparation expressing select MR1 recognizing T cell receptors from transgene expression vectors, the use of such T cell preparations in treatment of cancer, and to collections of MR1 reactive T cell receptor encoding nucleic acids and cells.