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公开(公告)号:US20220098552A1
公开(公告)日:2022-03-31
申请号:US17421840
申请日:2020-01-13
申请人: UNIVERSITÉ CÔTE D'AZUR , INSTITUT NATIONAL DE LA SANTÉ ET DE LA RECHERCHE MÉDICALE , CENTRE NATIONAL DE LA RECHERCHE SCIENTIFIQUE (CNRS) , CHU DE NICE
摘要: The method for tin vitro or ex vivo amplification of human adipose tissue stem cells includes: —extracting a stromal vascular fraction of a human adipose tissue including endothelial cells of the human adipose tissue vascular network and human adipose tissue stem cells, and an extracellular matrix of the human adipose tissue, the extracellular matrix including endothelial cells of the human adipose tissue vascular network, human adipose tissue stem cells and collagen; —mixing the stromal vascular fraction and the extracellular matrix; and—culturing the mixture obtained in the preceding step, in suspension, in a culture medium.