公开(公告)号：US20240151708A1

公开(公告)日：2024-05-09

申请号：US18281706

申请日：2022-03-24

申请人： UNIVERSITAT BERN

发明人： Alexander Eggel ,  Thomas Kaufmann ,  Noemi Zbaren

IPC分类号： G01N33/50 ,  C12N5/0787

CPC分类号： G01N33/5023 ,  C12N5/0642 ,  G01N33/5047 ,  C12N2501/2303 ,  C12N2501/999 ,  C12N2502/99 ,  C12N2503/00 ,  G01N2800/24

摘要： A method for producing non-human conditionally immortalized mast cell progenitors comprises: introducing a nucleic acid molecule comprising an inducible homeobox gene into myeloid progenitor cells, wherein said myeloid progenitor cells are derived from a non-human animal and are engineered to express a heterologous high-affinity IgE receptor alpha subunit (FcεRIα); and selecting for cells which contain the nucleic acid molecule. The non-human conditionally immortalized mast cell progenitors may be cultured to obtain differentiated mast cells. The mast cells find utility in assays for the determination of IgE mediated allergies.