公开(公告)号：US20240287457A1

公开(公告)日：2024-08-29

申请号：US18569895

申请日：2022-06-14

申请人： THE CHILDREN'S MEDICAL CENTER CORPORATION ,  INSTITUTE OF HEMATOLOGY AND BLOOD DISEASES HOSPITAL, CHINESE ACADEMY OF MEDICAL SCIENCES AND PEKING

发明人： Hongbo R. LUO ,  Yuping FAN ,  Fengxia MA ,  Yan TENG

IPC分类号： C12N5/0787 ,  A61K35/15 ,  A61P37/02

CPC分类号： C12N5/0642 ,  A61K35/15 ,  A61P37/02 ,  C12N2500/33 ,  C12N2501/22 ,  C12N2501/998 ,  C12N2501/999

摘要： The technology described herein is directed to compositions and cell culture media for increasing the lifespan of neutrophils. Also described are methods and kits for culturing neutrophils using the compositions or cell culture media. Such cultured neutrophils can be used to treat disorders such as neutropenia or microbial infections.

公开(公告)号：US20240151708A1

公开(公告)日：2024-05-09

申请号：US18281706

申请日：2022-03-24

申请人： UNIVERSITAT BERN

发明人： Alexander Eggel ,  Thomas Kaufmann ,  Noemi Zbaren

IPC分类号： G01N33/50 ,  C12N5/0787

CPC分类号： G01N33/5023 ,  C12N5/0642 ,  G01N33/5047 ,  C12N2501/2303 ,  C12N2501/999 ,  C12N2502/99 ,  C12N2503/00 ,  G01N2800/24

摘要： A method for producing non-human conditionally immortalized mast cell progenitors comprises: introducing a nucleic acid molecule comprising an inducible homeobox gene into myeloid progenitor cells, wherein said myeloid progenitor cells are derived from a non-human animal and are engineered to express a heterologous high-affinity IgE receptor alpha subunit (FcεRIα); and selecting for cells which contain the nucleic acid molecule. The non-human conditionally immortalized mast cell progenitors may be cultured to obtain differentiated mast cells. The mast cells find utility in assays for the determination of IgE mediated allergies.

公开(公告)号：US11759423B2

公开(公告)日：2023-09-19

申请号：US16479561

申请日：2017-12-15

申请人： Li Li ,  Xia Peng ,  Guogang Xie

发明人： Li Li ,  Xia Peng ,  Guogang Xie

IPC分类号： A61K9/127 ,  A61P37/06 ,  A61P37/08 ,  A61K35/15 ,  C12N5/0787 ,  C12N5/077

CPC分类号： A61K9/1271 ,  A61K9/1277 ,  A61K35/15 ,  A61P37/06 ,  A61P37/08 ,  C12N5/0642 ,  C12N5/0652

摘要： Provided are an exosome preparation formed by secretion by a mast cell cultured in vitro, a preparation method of an exosome therein, an exosome containing FcεRI protein on an outer surface thereof and in a substantially unbound state, and uses of the exosome preparation or exosome in a method for inhibiting mast cell activation in vitro and in preparing a drug for treating an IgE-mediated disease.

公开(公告)号：US20180214483A1

公开(公告)日：2018-08-02

申请号：US15747902

申请日：2016-08-05

申请人： Regents of the University of Minnesota ,  St. Jude Children's Research Hospital, Inc.

发明人： Bruce R. Blazar ,  Brent Koehn ,  Peter J. Murray ,  Jenny P.Y. Ting ,  Robert Zeiser ,  Jeff S. Miller

IPC分类号： A61K35/15 ,  A61P37/06 ,  A61K45/06 ,  C12N5/0787 ,  C12N5/0786 ,  A61K39/00 ,  C12N5/078

CPC分类号： A61K35/15 ,  A61K39/001 ,  A61K45/06 ,  A61K2035/122 ,  A61K2035/124 ,  A61K2039/515 ,  A61K2039/545 ,  A61K2039/577 ,  A61P37/06 ,  C12N5/0642 ,  C12N5/0645 ,  C12N5/0648 ,  C12N2501/22 ,  C12N2501/2313 ,  C12N2501/24

摘要： In one aspect, a method of treating a subject having or at risk of having graft-versus-host disease (GvHD) generally includes administering to the subject a plurality of myeloid-derived suppressor cells (MDSCs) effective to ameliorate at least one symptom or clinical sign of graft-versus-host disease compared to a suitable control subject. In another aspect, a method of treating a tumor in a subject generally includes administering to the subject an anti-tumor therapy and co-administering to the subject an inflammasome inciting agent in an amount effective to increase inflammasome activation of MDSCs sufficiently to reduce suppressor function of the MDSCs.

公开(公告)号：US09447379B2

公开(公告)日：2016-09-20

申请号：US13581432

申请日：2011-02-28

申请人： Reza Izadpanah ,  Eckhard Alt

发明人： Reza Izadpanah ,  Eckhard Alt

IPC分类号： C12N5/078 ,  C12N5/0786 ,  C12N5/0784 ,  C12N5/0787 ,  C12N5/0789 ,  C12N5/0793 ,  C12N15/85 ,  C12N15/88 ,  C12Q1/68

CPC分类号： C12N5/0647 ,  C12N5/0619 ,  C12N5/0639 ,  C12N5/0642 ,  C12N5/0645 ,  C12N15/85 ,  C12N15/88 ,  C12N2501/22 ,  C12N2501/2303 ,  C12N2506/1384 ,  C12Q1/6886

摘要： Methods and compositions for differentiating tissue resident multipotent mesenchmal stromal cells (MSCs) such as adipose tissue resident MSCs into a hematopoietic lineage are described.

摘要翻译： 描述了将组织驻留的多能间质基质细胞（MSC）如脂肪组织驻留的MSC分化成造血谱系的方法和组合物。

公开(公告)号：US20160060600A1

公开(公告)日：2016-03-03

申请号：US14427700

申请日：2013-09-11

申请人： INDUSTRY-UNIVERSITY COOPERATION FOUNDATION HANYANG

发明人： Eun Jung BAEK ,  Eun Mi LEE

IPC分类号： C12N5/078 ,  A61K38/17

CPC分类号： C12N5/0641 ,  A61K35/15 ,  A61K35/18 ,  A61K38/1709 ,  C12N5/0642 ,  C12N2500/99 ,  C12N2501/125 ,  C12N2501/14 ,  C12N2501/2303 ,  C12N2501/48 ,  C12N2501/998 ,  C12N2506/025 ,  C12N2506/11

摘要： The present invention relates to the in vitro production of erythrocytes/granulocytes and to the treatment of myelodysplastic syndrome using a method for inducing hemoblast differentiation. The present invention provides a media composition comprising gelsolin as an active ingredient for inducing the differentiation of hematopoietic precursor cells into erythrocytes/granulocytes, and a pharmaceutical composition comprising gelsolin as an active ingredient for treating myelodysplastic syndrome. Since the composition of the present invention improves the efficiency of differentiation of hematopoietic precursor cells into erythrocytes/granulocytes while maintaining a low rate of occurrence of cell dysplasia and having the effect of improving the enucleation rate and cell survivability, the present invention can be effectively used for producing erythrocytes/granulocytes in vitro and for treating myelodysplastic syndrome.

摘要翻译： 本发明涉及使用诱导成血细胞分化的方法体外生产红细胞/粒细胞和治疗骨髓增生异常综合征。 本发明提供了含有凝溶胶蛋白作为诱导将造血前体细胞分化为红细胞/粒细胞的活性成分的媒体组合物，以及含有凝溶胶蛋白作为治疗骨髓增生异常综合征的有效成分的药物组合物。 由于本发明的组合物提高了造血前体细胞分化为红细胞/粒细胞的效率，同时保持细胞发育不良的发生率低，并且具有提高摘除率和细胞存活率的效果，因此本发明可以有效地使用 用于在体外产生红细胞/粒细胞并用于治疗骨髓增生异常综合征。

公开(公告)号：US20150104820A1

公开(公告)日：2015-04-16

申请号：US13990991

申请日：2011-12-02

申请人： Tatsutoshi Nakahata ,  Kohichiro Tsuji ,  Feng Ma ,  Hirohisa Saito ,  Kenji Matsumoto

发明人： Tatsutoshi Nakahata ,  Kohichiro Tsuji ,  Feng Ma ,  Hirohisa Saito ,  Kenji Matsumoto

IPC分类号： C12N5/0787 ,  G01N33/50

CPC分类号： C12N5/0642 ,  C12N5/0696 ,  C12N2501/125 ,  C12N2501/145 ,  C12N2501/165 ,  C12N2501/20 ,  C12N2501/22 ,  C12N2501/2303 ,  C12N2501/2305 ,  C12N2501/2306 ,  C12N2501/26 ,  C12N2502/02 ,  C12N2506/02 ,  C12N2506/45 ,  G01N33/5029 ,  G01N33/5047 ,  G01N33/505 ,  G01N2500/04 ,  G01N2500/10

摘要： The present invention relates to a method for producing human eosinophils from human pluripotent stem cells. More specifically, the present invention provides a method for producing human eosinophils from human pluripotent stem cells, which method comprises the steps of: (1) co-culturing, in the presence of VEGF, human pluripotent stem cells with cells separated from the AGM region of a mammalian fetus; (2) performing suspension culture using a medium comprising IL-3, IL-6, Flt3 ligand, SCF, TPO and serum; (3) performing suspension culture using a medium comprising IL-3, SCF, GM-CSF and serum; and, optionally, (4) performing suspension culture using a medium comprising IL-3, IL-5 and serum.

摘要翻译： 本发明涉及从人多能干细胞生产人嗜酸性粒细胞的方法。 更具体地说，本发明提供从人多能干细胞生产人嗜酸性粒细胞的方法，该方法包括以下步骤：（1）在VEGF的存在下，将人多能干细胞与从AGM区域分离的细胞共培养 的哺乳动物胎儿; （2）使用包含IL-3，IL-6，Flt3配体，SCF，TPO和血清的培养基进行悬浮培养; （3）使用包含IL-3，SCF，GM-CSF和血清的培养基进行悬浮培养; 和（4）使用包含IL-3，IL-5和血清的培养基进行悬浮培养。

公开(公告)号：US20150044767A1

公开(公告)日：2015-02-12

申请号：US14523405

申请日：2014-10-24

申请人： KANEKA CORPORATION

发明人： Nobuyoshi UMEDA

IPC分类号： C12N5/0787

CPC分类号： G01N33/491 ,  A61M1/34 ,  C12N5/0087 ,  C12N5/0642

摘要： The present invention has an object to solve the problem in a method for separating nucleated cells from a cell-containing fluid using a cell separation filter. Specifically, the present invention provides a cell separation filter and a method for preparing cells using a cell separation filter, which are capable of reducing unnecessary cell contamination in a nucleated cell fraction without needs of designing non-woven fabrics to suit cell species to be recovered. The present invention also provides a cell separation filter and a method for preparing cells using the filter capable of improving the recovery yield of nucleated cells. The present invention provides a cell separation filter comprising: a container having an inlet and an outlet, an adsorbent filled in the container, and a partition having an opening, the partition separating the adsorbent. The present invention also provides a method for preparing nucleated cells including the steps of: introducing a cell-containing fluid through the inlet of the cell separation filter to contact the cell-containing fluid with the adsorbent; and recovering a nucleated cell fraction from the filter.

摘要翻译： 本发明的目的是解决使用细胞分离过滤器从含细胞液中分离有核细胞的方法中的问题。 具体地说，本发明提供了一种细胞分离过滤器和使用细胞分离过滤器制备细胞的方法，其能够减少有核细胞部分中不必要的细胞污染，而不需要设计非织造织物以适应要回收的细胞种类 。 本发明还提供一种细胞分离过滤器和使用能够提高有核细胞的回收率的过滤器来制备细胞的方法。 本发明提供了一种细胞分离过滤器，包括：具有入口和出口的容器，填充在容器中的吸附剂，以及具有开口的分隔件，隔板分隔吸附剂。 本发明还提供一种制备有核细胞的方法，包括以下步骤：通过细胞分离过滤器的入口引入含细胞的流体，以使细胞含有流体与吸附剂接触; 并从过滤器中回收有核细胞部分。

公开(公告)号：US08846395B2

公开(公告)日：2014-09-30

申请号：US12586600

申请日：2009-09-24

申请人： Igor I. Slukvin ,  Kyung-Dal Choi ,  Maksym A. Vodyanyk

发明人： Igor I. Slukvin ,  Kyung-Dal Choi ,  Maksym A. Vodyanyk

IPC分类号： C12N5/00 ,  C12N5/0787 ,  C12N5/0786 ,  C12N5/0784 ,  C12N5/078

CPC分类号： C12N5/0642 ,  C12N5/0639 ,  C12N5/0643 ,  C12N5/0645 ,  C12N2500/38 ,  C12N2500/90 ,  C12N2500/99 ,  C12N2501/15 ,  C12N2501/185 ,  C12N2501/22 ,  C12N2501/23 ,  C12N2501/25 ,  C12N2502/1394 ,  C12N2506/02 ,  C12N2506/45 ,  C12N2533/30

摘要： A method for efficient generation of neutrophils, eosinophils, macrophages, osteoclasts, dendritic cells an Langerhans cells from human embryonic stem cells is disclosed.

摘要翻译： 公开了一种从人胚胎干细胞中有效产生嗜中性粒细胞，嗜酸性粒细胞，巨噬细胞，破骨细胞，树突细胞的朗格汉斯细胞的方法。

公开(公告)号：US20110252487A1

公开(公告)日：2011-10-13

申请号：US13125090

申请日：2009-10-26

申请人： Kenji Nakanishi ,  Tomohiro Yoshimoto

发明人： Kenji Nakanishi ,  Tomohiro Yoshimoto

IPC分类号： A01K67/027 ,  C07K19/00 ,  G01N33/566 ,  C12N5/0783 ,  C12P21/00

CPC分类号： G01N33/505 ,  A61K39/39583 ,  A61K47/6901 ,  A61K49/0008 ,  A61K2039/5158 ,  A61K2039/57 ,  A61K2039/6056 ,  C12N5/0636 ,  C12N5/0642 ,  C12N2502/11 ,  G01N33/5047 ,  G01N33/5088 ,  G01N2800/122 ,  G01N2800/24 ,  A61K2300/00

摘要： The present invention provides a complex of an antigen and IgE binding to the antigen, a composition including an antigen and IgE binding to the antigen, and a method of using the complex or the composition. With the present invention, it is possible to induce naive T cells to develop into Th2 cells. Moreover, the present invention clarified a working mechanism of a Th2-type immune response, particularly a production mechanism of early IL-4. With use of the present invention, it is possible to provide a technique of treating and preventing Th2-type diseases.

摘要翻译： 本发明提供了与抗原结合的抗原和IgE的复合物，包含与抗原结合的抗原和IgE的组合物，以及使用该复合物或组合物的方法。 利用本发明，可以诱导幼稚T细胞发育成Th2细胞。 此外，本发明明确了Th2型免疫应答的工作机理，特别是早期IL-4的生产机制。 利用本发明，可以提供治疗和预防Th2型疾病的技术。