公开(公告)号：US20220206279A1

公开(公告)日：2022-06-30

申请号：US17644278

申请日：2021-12-14

Applicant: UNIVERSITY OF CENTRAL FLORIDA RESEARCH FOUNDATION, INC.

Inventor： Kyu Young Han ,  Benjamin Croop ,  Jialei Tang

IPC: G02B21/16 ,  G02B21/06 ,  G02B21/33 ,  G01N21/64

Abstract: A method and associated apparatus for generating instantaneous and uniform total internal reflection fluorescence (TIRF) excitation. An annular fiber bundle and is used with spatially incoherent light to provide appropriate illumination matched to parameters of the back focal plane of an oil-immersion or in-air imaging objective lens, enabling quantitative shadowless TIRF imaging.