公开(公告)号：US20220206279A1

公开(公告)日：2022-06-30

申请号：US17644278

申请日：2021-12-14

Applicant: UNIVERSITY OF CENTRAL FLORIDA RESEARCH FOUNDATION, INC.

Inventor： Kyu Young Han ,  Benjamin Croop ,  Jialei Tang

IPC: G02B21/16 ,  G02B21/06 ,  G02B21/33 ,  G01N21/64

Abstract: A method and associated apparatus for generating instantaneous and uniform total internal reflection fluorescence (TIRF) excitation. An annular fiber bundle and is used with spatially incoherent light to provide appropriate illumination matched to parameters of the back focal plane of an oil-immersion or in-air imaging objective lens, enabling quantitative shadowless TIRF imaging.

公开(公告)号：US20210325652A1

公开(公告)日：2021-10-21

申请号：US17236941

申请日：2021-04-21

Applicant: University of Central Florida Research Foundation, Inc.

Inventor： Jialei Tang ,  Vahid Ebrahimi ,  Kyu Young Han

IPC: G02B21/06 ,  G02B3/06 ,  G02B27/09 ,  G02B27/48 ,  G02B21/02 ,  G02B21/36 ,  G02B21/16 ,  G02B21/26 ,  G01N21/64

Abstract: An illumination system may include an illumination source, a line-projection system to simultaneously illuminate a pupil plane with a static distribution of a plurality of one-dimensional (1D) coherent beams. Each of the 1D coherent beams of the plurality of 1D coherent beams may extend lengthwise along a coherent direction between boundaries of the pupil plane, have a width along an incoherent direction perpendicular to the coherent direction, and are distributed in a parallel distribution along the incoherent direction. Each of the 1D coherent beams of the plurality of 1D coherent beams is also spatially coherent along the coherent direction and spatially incoherent along the incoherent direction. The system may further include an objective lens to form a light sheet in an imaging plane based on an incoherent superposition of the plurality of 1D coherent beams.

公开(公告)号：US11237370B2

公开(公告)日：2022-02-01

申请号：US16398630

申请日：2019-04-30

Applicant: University of Central Florida Research Foundation, Inc.

Inventor： Kyu Young Han ,  Jialei Tang

IPC: G01N21/64 ,  G02B21/00 ,  G02B21/36 ,  G02B21/02

Abstract: A dual inclined beam line-scanning confocal microscope apparatus, associated method, and applications thereof. An embodied dual-inclined beam line-scanning confocal microscope (2iLS) utilizes dual, parallel excitation beams each having a focused line shape that are scanned over a fluorescent sample. The emitted fluorescence from the sample is spatially filtered and detected by an array detector. 2iLS microscopy provides high resolution, ultrasensitivity, and deep optical sectioning capability. A reduced excitation intensity lowers photobleaching and photodamage.

公开(公告)号：US11231570B2

公开(公告)日：2022-01-25

申请号：US16398463

申请日：2019-04-30

Applicant: University of Central Florida Research Foundation, Inc.

Inventor： Kyu Young Han ,  Jialei Tang

IPC: G02B21/00 ,  G02B21/36 ,  G02B21/02 ,  G01N21/64

Abstract: A fluorescent emission HIST microscope utilizes a highly inclined tile beam that is scanned over a biological sample object. Fluorescence emission from the sample is propagated through a confocal slit into an sCMOS camera supporting a rolling shutter mode. The tile beam is synchronously swept with the readout of the camera to facilitate the rejection of background. The system provides for decoupling of the total imaging area from the beam thickness, which now solely depends on the width of the tile beam, enabling a thinner illumination and larger FOV imaging. Clear visualization of single molecules across a 130 μm×130 μm FOV provided a greater than 40× FOV than conventional HILO imaging. An associated imaging method is disclosed.

公开(公告)号：US20190331904A1

公开(公告)日：2019-10-31

申请号：US16398463

申请日：2019-04-30

Applicant: University of Central Florida Research Foundation, Inc.

Inventor： Kyu Young Han ,  JIALEI Tang

IPC: G02B21/00 ,  G02B21/36 ,  G02B21/02

Abstract: A fluorescent emission HIST microscope utilizes a highly inclined tile beam that is scanned over a biological sample object. Fluorescence emission from the sample is propagated through a confocal slit into an sCMOS camera supporting a rolling shutter mode. The tile beam is synchronously swept with the readout of the camera to facilitate the rejection of background. The system provides for decoupling of the total imaging area from the beam thickness, which now solely depends on the width of the tile beam, enabling a thinner illumination and larger FOV imaging. Clear visualization of single molecules across a 130 μm×130 μm FOV provided a greater than 40× FOV than conventional HILO imaging. An associated imaging method is disclosed.