公开(公告)号：US20200002771A1

公开(公告)日：2020-01-02

申请号：US16457067

申请日：2019-06-28

Applicant: UNIVERSITY OF CENTRAL FLORIDA RESEARCH FOUNDATION, INC.

Inventor： Kiminobu SUGAYA ,  Vaidya MANJUSHA

IPC: C12Q1/6886

Abstract: Disclosed herein are methods of detecting cancer in a patient that involve analyzing DNA in extracellular vesicles. In an exemplary embodiment, detection involves screening for an insert in a 3′ UTR of NANOGP8.

公开(公告)号：US20240399379A1

公开(公告)日：2024-12-05

申请号：US18697604

申请日：2022-09-28

Applicant: UNIVERSITY OF CENTRAL FLORIDA RESEARCH FOUNDATION, INC.

Inventor： Kiminobu SUGAYA ,  Vaidya MANJUSHA

IPC: B01L7/00 ,  C12Q1/6844

Abstract: Disclosed is a method to amplified nucleic acids by polymerase chain reaction (PCR) without isolation or purification from the biological samples. All the reactions associated with reverse transcriptase qPCR or real-time qPCR are completed on a filter paper chamber using a handheld Real-time PCR device. The process requires a very small amount of sample since there is no loss of materials associated with isolation and purification steps. The amplification process is faster than a normal tube-based PCR reaction because the thin chamber makes heating and cooling process fast. This technique is applicable in the rapid detection of pathogenic viruses from body fluids, such as COVID-19 detection in saliva. The technology is valuable for quantitative detection of nucleic acids that are established markers of diseases. Novel use of a piece of filter paper to perform sample collection, storage, and conduct direct PCR amplification without any extraction of the sample will allow us to test infections and the diseases anywhere by anybody without the requirement of technical knowledge or skills.