公开(公告)号：US20210388376A1

公开(公告)日：2021-12-16

申请号：US17361888

申请日：2021-06-29

Applicant: University of Manitoba

Inventor： Steve Whyard ,  Mark Belmonte

IPC: C12N15/82 ,  A01H5/00 ,  A01N37/46 ,  A01N63/60 ,  A01N63/30 ,  A01N57/16

Abstract: Provided herein are plants that reduce growth of a fungal pathogen, increase resistance of the plant to a fungal pathogen, or a combination thereof. The plant includes a polynucleotide that reduces expression of a coding region present in a fungal pathogen, such as Sclerotinia sclerotiorum or Botrytis cinerea. The polynucleotide can be present on the surface of the plant, expressed by a plant, or a combination thereof. Also provided are methods of making and methods of using the plants.

公开(公告)号：US10039287B2

公开(公告)日：2018-08-07

申请号：US15022759

申请日：2014-09-18

Applicant: UNIVERSITY OF MANITOBA

Inventor： Steve Whyard

IPC: C07H21/04 ,  A01N63/00 ,  C12N15/113 ,  A01K67/033

Abstract: Provided herein are modified insects having decreased expression of a testis-specific coding region compared to a control insect. In one embodiment, the modified insect includes the characteristic of reduced fertility, reduced fecundity, or a combination thereof, when compared to the control insect. Optionally and preferably, the competiveness of the modified insect is not significantly reduced compared to the control insect. Also included herein are methods for making a modified insect, and methods for using the modified insects, including making populations of modified insects and use of the modified insects in sterile insect technique for biological control.

公开(公告)号：US20220225622A1

公开(公告)日：2022-07-21

申请号：US17605788

申请日：2020-04-14

Applicant: University of Manitoba

Inventor： Steve Whyard

IPC: A01N63/60

Abstract: Flea beetles (species of the genus Phyllotreta) are serious pests of cruciferous crops, causing millions of dollars losses each year, despite the use of chemical pesticides. Our current pesticides are broad-spectrum in their activity, killing not just the pests, but also many non-target species, including beneficial insects such as pollinators and predators. Here, an alternative set of pesticides, based on double-stranded RNA (dsRNA), is shown to be effective at killing flea beetles when the dsRNAs are applied to a leaf surface and fed to the insects. Insects that fed on leaves sprayed with the dsRNAs died within 8 days of first exposure, but also showed reduced feeding activity within several days, thereby reducing feeding damage to the plants. Importantly, the dsRNAs were designed to be specific for the flea beetles, and when delivered to non-target beetles, no adverse effects were observed, illustrating the specificity of this new type of pesticide.

公开(公告)号：US20190153467A1

公开(公告)日：2019-05-23

申请号：US16098387

申请日：2017-05-03

Applicant: University of Manitoba

Inventor： Steve Whyard ,  Mark Belmonte

IPC: C12N15/82 ,  A01N63/04 ,  A01N57/16

Abstract: Provided herein are plants that reduce growth of a fungal pathogen, increase resistance of the plant to a fungal pathogen, or a combination thereof. The plant includes a polynucleotide that reduces expression of a coding region present in a fungal pathogen, such as Sclerotinia sclerotiorum or Botrytis cinerea. The polynucleotide can be present on the surface of the plant, expressed by a plant, or a combination thereof. Also provided are methods of making and methods of using the plants.

公开(公告)号：US20230255212A1

公开(公告)日：2023-08-17

申请号：US18005118

申请日：2021-07-05

Applicant: University of Manitoba

Inventor： Steve Whyard

IPC: A01N63/60 ,  C12N15/113

CPC classification number: A01N63/60 ,  C12N15/113 ,  C12N2310/14 ,  C12N2310/531

Abstract: Described herein is a “paperclip” structured dsRNA (pcRNA) that has two closed ends (FIG. 1), and was developed and tested for its ability to enter insect cells and induce RNAi. While conventional dsRNAs, with their two collinear RNA strands, enter insect cells by clathrin-mediated endocytosis, the pcRNAs can enter cells by a clathrin-independent mechanism. The new structured dsRNA can be used to deliver dsRNA to insects that either develop resistance through alterations to their conventional uptake mechanisms and to insects that are naturally refractory to dsRNAs, including lepidopteran pests.

公开(公告)号：US11085052B2

公开(公告)日：2021-08-10

申请号：US16098387

申请日：2017-05-03

Applicant: University of Manitoba

Inventor： Steve Whyard ,  Mark Belmonte

IPC: C12N15/82 ,  A01H5/00 ,  A01N37/46 ,  A01N63/30 ,  A01N57/16

Abstract: Provided herein are plants that reduce growth of a fungal pathogen, increase resistance of the plant to a fungal pathogen, or a combination thereof. The plant includes a polynucleotide that reduces expression of a coding region present in a fungal pathogen, such as Sclerotinia sclerotiorum or Botrytis cinerea. The polynucleotide can be present on the surface of the plant, expressed by a plant, or a combination thereof. Also provided are methods of making and methods of using the plants.