公开(公告)号：US20160215034A1

公开(公告)日：2016-07-28

申请号：US15025283

申请日：2014-09-24

Applicant: UNIVERSITY OF ULSAN FOUNDATION FOR INDUSTRY COOPERATION

Inventor： Han CHOE ,  Han Bong RYU ,  Bich Hang DO

IPC: C07K14/535 ,  C07K2/00

CPC classification number: C07K14/535 ,  C07K2/00 ,  C07K2319/21

Abstract: The present invention relates to a water-soluble expression and a purification method of a human granulocyte colony-stimulating factor (hGCSF) recombinant protein having biological activities using an N-terminal tag, and provides a recombinant expression vector containing a solubility enhancing tag and a hGCSF gene. In addition, provided are: a recombinant microorganism transformed with the recombinant expression vector; a method for producing a hGCSF recombinant protein (rhGCSF); and a rhGCSF produced by the production method.

Abstract translation: 本发明涉及使用N-末端标签具有生物活性的人粒细胞集落刺激因子（hGCSF）重组蛋白质的水溶性表达和纯化方法，并提供含有溶解性增强标签和 hGCSF基因。 此外，提供：用重组表达载体转化的重组微生物; 生产hGCSF重组蛋白（rhGCSF）的方法; 和通过生产方法生产的rhGCSF。