公开(公告)号：US20160130555A1

公开(公告)日：2016-05-12

申请号：US14897403

申请日：2014-06-12

Applicant: UNIVERSITY OF WASHINGTON THROUGH ITS CENTER FOR COMMERCIALIZATION

Inventor： Hannele RUOHOLA-BAKER ,  Kavitha KUPPUSAMY ,  Henrik SPERBER

IPC: C12N5/077 ,  A61K35/34 ,  C12N15/113

CPC classification number: C12N5/0657 ,  A61K35/34 ,  C12N15/111 ,  C12N15/113 ,  C12N2310/141 ,  C12N2320/30

Abstract: Described herein are methods and compositions useful for inducing maturation of a cardiomyocyte to a mature (e.g., adult) phenotype, such that the function and morphology of the mature cardiomyocyte matches or more closely mimics that of the adult heart. The methods and compositions use Let-7 miRNAs and modified forms thereof. Such methods and compositions permit the study and treatment of adult-onset cardiac diseases, disorders or injuries with mature cardiomyocytes that mimic the heart function of an adult. Methods of using cardiomyocytes matured in this manner for drug identification and drug cardiotoxicity testing are also provided.

Abstract translation: 本文描述的是用于诱导成熟（例如成人）表型的心肌细胞成熟的方法和组合物，使得成熟心肌细胞的功能和形态与成人心脏的功能和形态相匹配或更接近。 方法和组合物使用Let-7 miRNA及其修饰形式。 这些方法和组合物允许研究和治疗成人心脏病，成熟心肌细胞的障碍或损伤，其模拟成人的心脏功能。 还提供了以这种方式成熟的心肌细胞用于药物鉴定和药物心脏毒性测试的方法。