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公开(公告)号:US09670495B2
公开(公告)日:2017-06-06
申请号:US14770074
申请日:2014-02-25
发明人: Maitreya Dunham , Ivan Liachko
CPC分类号: C12N15/81 , C12N15/69 , C12N15/815
摘要: A DNA sequence that functions as an origin in many different yeast species. From 1 to 17 mutations can be introduced into this sequence to improve its function across multiple yeasts. The resulting synthetic DNA sequence confers stable plasmid replication function in all yeast species tested, including but not limited to Saccharomyces cerevisiae, Lachancea kluyveri, Kluyveromyces lactis, Kluyveromyces wickerhammii, Hansenula polymorpha, and Pichia pastoris. Also provided are sequences that function as an optimal origin in the industrially useful Pichia pastoris.