公开(公告)号：US4289756A

公开(公告)日：1981-09-15

申请号：US860570

申请日：1977-12-14

申请人： Ulrich Zimmermann ,  Gunter Pilwat ,  Karin Bock ,  Hermann J. Buers

发明人： Ulrich Zimmermann ,  Gunter Pilwat ,  Karin Bock ,  Hermann J. Buers

IPC分类号： A61K9/50 ,  A61K35/12 ,  A61K35/18 ,  A61K37/48 ,  A61K35/14 ,  A61K43/00

CPC分类号： A61K9/5068 ,  A61K35/12 ,  A61K35/18 ,  Y10T436/101666 ,  Y10T436/107497 ,  Y10T436/108331

摘要： Loaded cells suspended in a physiological solution are prepared in a process in which their volume is expanded by osmotic pressure. Cells so prepared suspended in a physiological solution, when injected into the bloodstream of a living body are preferentially accumulated in the spleen and liver of the body, which treats them as foreign bodies, even though the original cells may have been cells of an animal of the same species. Similarly by storing the loaded cells in a solution containing substances such as glutadialdehyde, formaldehyde that react with the protein phase of membranes or which, like difluoro dinitrobenzene, react with the lipid layer thereof, the loaded cells can also be caused to be broken down by the spleen and/or liver. Premature release of the contents of the loaded cells is prevented and timely release of such contents is assured by incorporating other materials into the loaded cells during the preparation process in accordance with the related application of the same inventors, Ser. No. 859,240 filed Dec. 9, 1977, now U.S. Pat. No. 4,224,313, issued Sept. 23, 1980.

摘要翻译： 在其通过渗透压膨胀其体积的过程中制备悬浮在生理溶液中的负载细胞。 悬浮在生理溶液中的细胞在注射到生物体的血液中时优先蓄积在身体的脾脏和肝脏中，即将其视为异物，尽管原始细胞可能是动物的细胞 相同的物种 类似地，通过将​​负载的细胞储存在含有诸如谷丙二醛，甲醛与膜的蛋白质相反应的物质的溶液中，或者像二氟二硝基苯一样与其脂质层反应的负载细胞也可以被分解， 脾脏和/或肝脏。 防止负载的电池的内容物的过早释放，根据相同发明人的相关申请，在制备过程中，通过将其它材料并入加载的电池中来确保这些内容物的及时释放。 1977年12月9日提交的第859,240号，现为美国专利 1980年9月23日发布的第4,224,313号。