公开(公告)号：US5869335A

公开(公告)日：1999-02-09

申请号：US519599

申请日：1995-08-25

申请人： Ulrike G. Munderloh ,  Timothy J. Kurtti ,  Katherine M. Kocan ,  Edmour F. Blouin ,  Sidney A. Ewing

发明人： Ulrike G. Munderloh ,  Timothy J. Kurtti ,  Katherine M. Kocan ,  Edmour F. Blouin ,  Sidney A. Ewing

IPC分类号： A61K39/02 ,  C07K14/29 ,  C12N1/00

CPC分类号： C07K14/29 ,  A61K39/0233

摘要： The invention is directed to methods of culturing rickettsiae in Ixodes scapularis cell lines. The methods of the invention provide for culture of microorganisms such as Anaplasma marginale, Ehrlichia canis, and Rickettsia rickettsii. A method of the invention involves incubating a rickettsia with an Ixodes scapularis tick cell culture in a culture medium under reduced oxygen and increased CO.sub.2 at a sufficient temperature until growth of the rickettsia is detected. The culture medium comprises a medium suitable for the growth of invertebrate cells supplemented with an organic buffer. The cell culture method can be used in large scale production of rickettsia containing products useful in diagnostic assays and vaccine preparations.

摘要翻译： 本发明涉及在sc in is细胞系中培养立克次氏体的方法。 本发明的方法提供了微生物的培养物，例如ma as margin，艾氏杆菌和立克次体体积立克次氏体。 本发明的方法包括在减压氧培养基中培养立克次体和肩胛骨蜱细胞培养物，并在足够的温度下升高CO 2，直到检测到立克次体的生长。 培养基包括适合于补充有机缓冲液的无脊椎动物细胞生长的培养基。 细胞培养方法可用于大规模生产含有诊断测定和疫苗制剂的立克次体含有产品。