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    Method of growing rickettsiae in Ixodes scapularis tick cell culture and preparing antigens and vaccines of rickettsiae
    2.
    发明授权
    Method of growing rickettsiae in Ixodes scapularis tick cell culture and preparing antigens and vaccines of rickettsiae 失效
    在sc is蜱细胞培养中生长立克次体的方法，并制备立克次体的抗原和疫苗

    公开(公告)号：US5869335A

    公开(公告)日：1999-02-09

    申请号：US519599

    申请日：1995-08-25

    申请人： Ulrike G. Munderloh ,  Timothy J. Kurtti ,  Katherine M. Kocan ,  Edmour F. Blouin ,  Sidney A. Ewing

    发明人： Ulrike G. Munderloh ,  Timothy J. Kurtti ,  Katherine M. Kocan ,  Edmour F. Blouin ,  Sidney A. Ewing

    IPC分类号： A61K39/02 ,  C07K14/29 ,  C12N1/00

    CPC分类号： C07K14/29 ,  A61K39/0233

    摘要： The invention is directed to methods of culturing rickettsiae in Ixodes scapularis cell lines. The methods of the invention provide for culture of microorganisms such as Anaplasma marginale, Ehrlichia canis, and Rickettsia rickettsii. A method of the invention involves incubating a rickettsia with an Ixodes scapularis tick cell culture in a culture medium under reduced oxygen and increased CO.sub.2 at a sufficient temperature until growth of the rickettsia is detected. The culture medium comprises a medium suitable for the growth of invertebrate cells supplemented with an organic buffer. The cell culture method can be used in large scale production of rickettsia containing products useful in diagnostic assays and vaccine preparations.

    摘要翻译： 本发明涉及在sc in is细胞系中培养立克次氏体的方法。 本发明的方法提供了微生物的培养物，例如ma as margin，艾氏杆菌和立克次体体积立克次氏体。 本发明的方法包括在减压氧培养基中培养立克次体和肩胛骨蜱细胞培养物，并在足够的温度下升高CO 2，直到检测到立克次体的生长。 培养基包括适合于补充有机缓冲液的无脊椎动物细胞生长的培养基。 细胞培养方法可用于大规模生产含有诊断测定和疫苗制剂的立克次体含有产品。

    Recombinant antigen MSP1a from anaplasma marginale to reduce infections in ticks, vaccine compositions and methods of use
    4.
    发明授权
    Recombinant antigen MSP1a from anaplasma marginale to reduce infections in ticks, vaccine compositions and methods of use 失效
    重组抗原MSP1a来自不定形的margin ale，以减少蜱感染，疫苗组合物和使用方法

    公开(公告)号：US06979451B1

    公开(公告)日：2005-12-27

    申请号：US10285319

    申请日：2002-10-31

    申请人： Jose de Jesus de la Fuente ,  Katherine M. Kocan ,  Jose Carlos Garcia-Garcia ,  Edmour F. Blouin

    发明人： Jose de Jesus de la Fuente ,  Katherine M. Kocan ,  Jose Carlos Garcia-Garcia ,  Edmour F. Blouin

    IPC分类号： A01N63/00 ,  A01N65/00 ,  A61K39/00 ,  A61K39/002 ,  A61K39/005 ,  A61K39/02 ,  A61K39/38 ,  C07K14/195

    CPC分类号： A61K39/0233 ,  A61K39/00 ,  C07K14/195 ,  Y02A50/403

    摘要： Vaccines and methods for inducing an immune response in a ruminant. The vaccine composition including pathogen and tick-derived antigens and a carrier or diluent. The method for inducing an immune response in a ruminate to provide immune protection which reduces the infection of ticks by A. marginale and/or prevents the transmission of the pathogen includes the steps of administering to the ruminant an effective amount of the vaccine composition having at least one antigen member of the group comprising at least one antigen member of the group comprising (i) recombinant MSP1a surface protein antigen of A. marginale, (ii) a subunit of recombinant MSP1a surface protein antigen of A. marginale and (iii) recombinant MSP1a surface protein antigen or subunits thereof in combination with antigen preparation derived from A. marginale infected cultured tick IDE8 cells and/or other pathogen and tick-derived antigens, and a carrier or diluent.

    摘要翻译： 在反刍动物中诱导免疫应答的疫苗和方法。 疫苗组合物包括病原体和蜱衍生的抗原以及载体或稀释剂。 用于在反刍动物中诱导免疫应答以提供免疫保护的方法，其减少虱子的感染，并且/或防止病原体的传播包括以下步骤：向反刍动物施用有效量的疫苗组合物，其具有 该组的至少一个抗原成员包括至少一个抗原成员，其包含（i）重要的MSP1a表面蛋白抗原，（ii）重要的MSP1a表面蛋白抗原亚单位和（iii）重组 MSP1a表面蛋白抗原或其亚基与衍生自A.Repale感染的培养的蜱IDE8细胞和/或其他病原体和蜱衍生抗原的抗原制剂，以及载体或稀释剂组合。