公开(公告)号：US20120149111A1

公开(公告)日：2012-06-14

申请号：US13309280

申请日：2011-12-01

申请人： Ulrike G.K. WEGST ,  Margaret WHEATLEY ,  Benjamin W. RIBLETT ,  Nicola FRANCIS ,  Amalie Elizabeth DONIUS

发明人： Ulrike G.K. WEGST ,  Margaret WHEATLEY ,  Benjamin W. RIBLETT ,  Nicola FRANCIS ,  Amalie Elizabeth DONIUS

IPC分类号： C12N5/02 ,  B29C35/16

CPC分类号： C12N5/0619 ,  C12N2533/32 ,  C12N2533/52 ,  C12N2533/54 ,  C12N2533/72 ,  C12N2533/74 ,  C12N2537/00

摘要： The present invention relates to scaffolds that can physically guide cells, e.g. neurons, while best matching the material properties of native tissue. The present invention also relates to methods of generating such scaffolds, and for the use of such scaffolds, e.g. in spinal cord and peripheral nerve injury repair. The methods of the present invention include a uniquely controlled freeze casting process to generate highly porous, linearly oriented scaffolds. The scaffolds of the present invention not only comprise a highly aligned porosity, but also contain secondary guidance structures in the form of ridges running parallel to the pores to create a series of microstructured and highly aligned channels. This hierarchy of structural guidance aligns and guides neurite outgrowth down the channels created by the ridges, and keep neurites from branching perpendicular to the inter-ridge grooves.

摘要翻译： 本发明涉及能够物理引导细胞的支架，例如， 神经元，同时最好地匹配天然组织的材料性质。 本发明还涉及产生这种支架的方法，以及用于这种支架的使用。 在脊髓和周围神经损伤修复。 本发明的方法包括独特控制的冷冻铸造方法以产生高度多孔的，线性定向的支架。 本发明的支架不仅包括高度对准的孔隙率，而且还包含平行于孔的脊的形式的次级引导结构，以产生一系列微结构和高度排列的通道。 这种结构指导层次结构使脊突产生的神经突向下生长，并使脊突变成垂直于脊间沟的分支。