公开(公告)号：US20240352414A1

公开(公告)日：2024-10-24

申请号：US18633066

申请日：2024-04-11

申请人： Washington University

发明人： Andrew Yoo ,  Zhao Sun

IPC分类号： C12N5/0793 ,  C12N15/113 ,  C12N15/86 ,  G01N33/50

CPC分类号： C12N5/0619 ,  C12N15/113 ,  C12N15/86 ,  G01N33/5023 ,  G01N33/5058 ,  C12N2310/14 ,  C12N2501/60 ,  C12N2506/1307 ,  C12N2513/00

摘要： Provided herein are methods of making and using reprogrammed neuronal cells and neuronal spheroids from patient-derived somatic cells in 3D-cultures, by expressing at least one neurogenic miRNA and optionally at least one neurogenic transcription factor.

公开(公告)号：US20240327796A1

公开(公告)日：2024-10-03

申请号：US18606805

申请日：2024-03-15

申请人： MEMORIAL SLOAN-KETTERING CANCER CENTER

发明人： Lorenz STUDER ,  Bastian ZIMMER ,  Jason TCHIEU

IPC分类号： C12N5/0775 ,  A61K35/12 ,  C12N5/00 ,  C12N5/0735 ,  C12N5/074 ,  C12N5/079 ,  C12N5/0793

CPC分类号： C12N5/0667 ,  C12N5/0018 ,  C12N5/0606 ,  C12N5/0618 ,  C12N5/0619 ,  C12N5/0696 ,  A61K35/12 ,  C12N2501/01 ,  C12N2501/115 ,  C12N2501/155 ,  C12N2501/16 ,  C12N2501/727 ,  C12N2501/999 ,  C12N2506/02 ,  C12N2506/03 ,  C12N2506/04 ,  C12N2510/00 ,  C12N2533/52

摘要： The presently disclosed subject matter provides for in vitro methods of inducing differentiation of human stem cells into neural crest, cranial placode or non-neuro ectoderm precursors, and cells generated by such methods. The presently disclosed subject matter also provides for uses of such cells for treating neurodegenerative and pituitary disorders.

公开(公告)号：US20240327789A1

公开(公告)日：2024-10-03

申请号：US18625740

申请日：2024-04-03

申请人： University of Southern California

发明人： Alexander Atamian ,  Giorgia Quadrato

IPC分类号： C12N5/0793 ,  C12N5/00 ,  G01N33/50

CPC分类号： C12N5/0619 ,  C12N5/0018 ,  G01N33/5014 ,  G01N33/5023 ,  G01N33/5058 ,  G01N33/5088 ,  C12N2500/25 ,  C12N2500/40 ,  C12N2500/44 ,  C12N2501/119 ,  C12N2501/13 ,  C12N2501/21 ,  C12N2501/395 ,  C12N2501/91 ,  C12N2501/999 ,  C12N2503/02 ,  C12N2506/02 ,  C12N2506/45 ,  C12N2513/00 ,  C12N2527/00

摘要： We report human cerebellar organoids which recapitulate the major milestones of cerebellar development including generating bona fide Purkinje cells, as well as methods of making and using these human cerebellar organoids such as uses in drug target identification and drug screening. In various embodiments, we report a human organoid model (human cerebellar organoids [hCerOs]) capable of developing the complex cellular diversity of the fetal cerebellum, including a human-specific rhombic lip progenitor population that have never been generated in vitro prior to our study. 2-month-old hCerOs form distinct cytoarchitectural features, including laminar organized layering, and create functional connections between inhibitory and excitatory neurons that display coordinated network activity. Long-term culture of hCerOs allows healthy survival and maturation of Purkinje cells that display molecular and electrophysiological hallmarks of their in vivo counterparts, addressing a long-standing challenge in the field.

公开(公告)号：US20240309320A1

公开(公告)日：2024-09-19

申请号：US18576909

申请日：2022-07-08

申请人： THE BROAD INSTITUTE, INC. ,  MASSACHUSETTS INSTITUTE OF TECHNOLOGY

发明人： Feng Zhang ,  Julia Joung

IPC分类号： C12N5/0793 ,  A61K35/30 ,  C12Q1/6809 ,  C12Q1/6881

CPC分类号： C12N5/0619 ,  A61K35/30 ,  C12Q1/6809 ,  C12Q1/6881 ,  C12N2510/00 ,  C12Q2600/158

摘要： The subject matter disclosed herein is generally directed to methods of differentiating pluripotent cells into target cell types and screening platforms for systematically identifying transcription factors (TFs) that drive differentiation of pluripotent cells into target cell types. Also disclosed is a high-throughput multiplex screening platform. Also disclosed are in vitro models for neural progenitor cells and cardiomyocytes.

公开(公告)号：US12060573B2

公开(公告)日：2024-08-13

申请号：US16069774

申请日：2017-01-17

申请人： AMORPHICAL LTD.

发明人： Yosef Ben ,  Abraham Shahar ,  Amir Arav

IPC分类号： C12N5/00 ,  C12N5/073 ,  C12N5/075 ,  C12N5/076 ,  C12N5/077 ,  C12N5/0775 ,  C12N5/0793

CPC分类号： C12N5/0018 ,  C12N5/0604 ,  C12N5/0609 ,  C12N5/061 ,  C12N5/0619 ,  C12N5/0658 ,  C12N5/0663 ,  C12N2500/14

摘要： Stabilized amorphous calcium carbonate (ACC) as a supplement of cell culture media and the cell culture medium supplements comprising stabilized ACC are provided. In particular the stabilized ACC is useful for enhancing the growth of cell and tissue cultures, gametes and embryos in vitro.

公开(公告)号：US20240263134A1

公开(公告)日：2024-08-08

申请号：US18591734

申请日：2024-02-29

申请人： MEMORIAL SLOAN-KETTERING CANCER CENTER

发明人： Lorenz Studer ,  Jae-Won Shim ,  Sonja Kriks

IPC分类号： C12N5/0793 ,  A61K35/30 ,  C12N5/0797

CPC分类号： C12N5/0619 ,  A61K35/30 ,  C12N5/0623 ,  C12N2501/01 ,  C12N2501/119 ,  C12N2501/13 ,  C12N2501/15 ,  C12N2501/16 ,  C12N2501/41 ,  C12N2501/415 ,  C12N2501/999 ,  C12N2506/02 ,  C12N2506/45

摘要： The present invention relates to the field of stem cell biology, in particular the linage specific differentiation of pluripotent or multipotent stem cells, which can include, but is not limited to, human embryonic stem cells (hESC) in addition to nonembryonic human induced pluripotent stem cells (hiPSC), somatic stem cells, stem cells from patients with a disease, or any other cell capable of lineage specific differentiation. Specifically described are methods to direct the lineage specific differentiation of hESC and/or hiPSC into floor plate midbrain progenitor cells and then further into large populations of midbrain fate FOXA2+LMX1A+TH+ dopamine (DA) neurons using novel culture conditions. The midbrain fate FOXA2+LMX1A+TH+ dopamine (DA) neurons made using the methods of the present invention are further contemplated for various uses including, but not limited to, use in in vitro drug discovery assays, neurology research, and as a therapeutic to reverse disease of, or damage to, a lack of dopamine neurons in a patient. Further, compositions and methods are provided for differentiating midbrain fate FOXA2+LMX1A+TH+ dopamine (DA) neurons from human pluripotent stem cells for use in disease modeling, in particular Parkinson's disease. Additionally, authentic DA neurons are enriched for markers, such as CD142, and A9 type neuronal cells.

公开(公告)号：US20240238445A1

公开(公告)日：2024-07-18

申请号：US18552290

申请日：2022-07-08

申请人： NEURACLE SCIENCE CO., LTD. ,  KOREA UNIVERSITY RESEARCH AND BUSINESS FOUNDATION ,  KOREA UNIVERSITY RESEARCH AND BUSINESS FOUNDATION, SEJONG CAMPUS

发明人： Jae Young SEONG ,  Youngjoo BYUN ,  Hoyun KWAK ,  Sitaek OH ,  Min-Hyeok LEE ,  Yongwoo JEONG ,  Nui HA ,  Eun-Ho CHO ,  Suhyun LEE ,  Sang-Myeong LEE ,  Yerim LEE ,  Eun Bee CHO ,  Jae Keun LEE ,  Han-Byul KIM ,  Soon-gu KWON

IPC分类号： A61K48/00 ,  A61K31/055 ,  A61K31/085 ,  A61K31/136 ,  A61K31/137 ,  A61K38/08 ,  A61K38/10 ,  A61K38/17 ,  A61K45/06 ,  A61K47/64 ,  A61P25/02 ,  A61P25/28 ,  C12N5/0793

CPC分类号： A61K48/005 ,  A61K31/055 ,  A61K31/085 ,  A61K31/136 ,  A61K31/137 ,  A61K38/08 ,  A61K38/10 ,  A61K38/1709 ,  A61K45/06 ,  A61K47/64 ,  A61P25/02 ,  A61P25/28 ,  C12N5/0619

摘要： The present disclosure provides mimic molecules that are capable of specifically targeting a FAM19A5 protein, and thereby, inhibiting, reducing, and/or dissociating the interaction between members of the LRRC4 protein family and the FAM19A5 protein. The present disclosure also provides methods for promoting neurite outgrowth, by administering a mimic molecule described herein.

公开(公告)号：US20240238254A1

公开(公告)日：2024-07-18

申请号：US18563800

申请日：2022-05-24

申请人： The Johns Hopkins University ,  The University of Southern California

发明人： Gabsang LEE ,  Hyungjin EOH ,  Hojae LEE ,  Thomas LLOYD ,  Nicholas J. MARAGAKIS

IPC分类号： A61K31/415 ,  A61K31/05 ,  A61K31/192 ,  A61K31/352 ,  A61P25/28 ,  C12N5/0793 ,  C12Q1/6809 ,  C12Q1/6883

CPC分类号： A61K31/415 ,  A61K31/05 ,  A61K31/192 ,  A61K31/352 ,  A61P25/28 ,  C12N5/0619 ,  C12Q1/6809 ,  C12Q1/6883 ,  C12N2500/38 ,  C12N2501/13 ,  C12N2501/41 ,  C12N2501/999 ,  C12N2506/02 ,  C12N2506/45 ,  C12Q2600/158

摘要： Embodiments disclosed relate to methods of treating amyotrophic lateral sclerosis (ALS), including: selecting a therapeutic compound: treating an aberrant arachidonic acid (AA) metabolic pathway in an ALS cell.

公开(公告)号：US20240226605A9

公开(公告)日：2024-07-11

申请号：US18335541

申请日：2023-06-15

申请人： Arizona Board of Regents on Behalf of Arizona State University

发明人： William James P. TYLER

IPC分类号： A61N7/00 ,  A61B5/00 ,  A61B5/369 ,  A61B5/372 ,  A61N5/06 ,  C12N5/0793 ,  C12N13/00

CPC分类号： A61N7/00 ,  A61B5/0036 ,  A61B5/369 ,  A61B5/372 ,  A61B5/4836 ,  A61N5/062 ,  A61N5/0622 ,  C12N5/0619 ,  C12N13/00 ,  A61N5/0613 ,  A61N2005/0651 ,  A61N5/067 ,  A61N2007/0026 ,  A61N2007/0078

摘要： The present invention comprises methods and devices for modulating the activity or activities of living cells, such as cells found in or derived from humans, animals, plants, insects, microorganisms and other organisms. Methods of the present invention comprise use of the application of ultrasound, such as low intensity, low frequency ultrasound, to living cells to affect the cells and modulate the cells' activities. Devices of the present invention comprise one or more components for generating ultrasound waves, such as ultrasonic emitters, transducers or piezoelectric transducers, composite transducers, CMUTs, and which may be provided as single or multiple transducers or in an array configurations. The ultrasound waves may be of any shape, and may be focused or unfocused.

公开(公告)号：US20240218362A1

公开(公告)日：2024-07-04

申请号：US18323956

申请日：2023-05-25

申请人： The Regents of the University of California

发明人： Xiang-Dong Fu ,  Hao Qian

IPC分类号： C12N15/113 ,  A61P25/16 ,  C12N5/0793

CPC分类号： C12N15/113 ,  A61P25/16 ,  C12N5/0619 ,  C12N2310/11 ,  C12N2310/14 ,  C12N2310/531

摘要： Provided herein is a method of reprogramming a non-neuronal cell to a neuron. Aspects of the present disclosure relate to using cell reprogramming agent suppresses the expression or activity of PTB to convert a non-neuronal cell into a neuron. Also provided herein is a method of treating neurodegenerative disease by reprogramming non-neuronal cells in vivo to functional neurons.