公开(公告)号：US12195797B2

公开(公告)日：2025-01-14

申请号：US18737230

申请日：2024-06-07

Applicant: Ultima Genomics, Inc.

Inventor： Daniel Mazur

IPC: C12Q1/68 ,  C12Q1/6844 ,  C12Q1/6869

Abstract: The present disclosure provides compositions, methods, and systems for preparing nucleic acid samples for sequencing, including attaching adapters to a template nucleic acid molecule having a first and second strands; attaching the template nucleic acid molecule to a support; detaching the first and second strands from each other; generating a reverse complement copy of the second strand using a primer coupled to the support; generating amplified copies of the first strand and the reverse complement copy of the second strand using primers coupled to the support; and identifying the sequences of the first strand and the reverse complement copy of the second strand. Further provided herein are methods of error correction by preserving both strands of a template nucleic acid molecule during amplification.