公开(公告)号：US20160194607A1

公开(公告)日：2016-07-07

申请号：US14989310

申请日：2016-01-06

申请人： Université Pierre et Marie Curiè (Paris 6) ,  Etablissement Français du Sang ,  ASSISTANCE PUBLIQUE - HOPITAUX DE PARIS

发明人： Luc Douay ,  Marie-Catherine Giarratana

IPC分类号： C12N5/0789

CPC分类号： C12N5/0647 ,  C12N5/0641 ,  C12N2500/25 ,  C12N2500/90 ,  C12N2501/125 ,  C12N2501/14 ,  C12N2501/145 ,  C12N2501/155 ,  C12N2501/165 ,  C12N2501/2303 ,  C12N2501/2306 ,  C12N2501/26 ,  C12N2501/91

摘要： A method for the production of cells of the hematopoietic lineage includes culturing hematopoietic stem cells (HSC) or embryonic bodies with a cell culture medium (the cell culture medium includes insulin at a concentration of from 1 to 50 μg/ml, transferrin at a concentration of from 100 μg/ml to 2000 μg/ml and plasma or serum at a concentration of from 1% to 30%) under conditions allowing producing cells of the hematopoietic lineage; and collecting cells of the hematopoietic lineage.

摘要翻译： 用于制造造血谱系细胞的方法包括用细胞培养基培养造血干细胞（HSC）或胚胎体（细胞培养基包括浓度为1至50μg/ ml的胰岛素，浓度为的转铁蛋白 在100μg/ ml至2000μg/ ml的浓度范围内，血浆或血清浓度为1％至30％），在允许产生造血谱系细胞的条件下; 并收集造血谱系的细胞。