公开(公告)号：US20230381778A1

公开(公告)日：2023-11-30

申请号：US18245947

申请日：2021-09-24

Applicant: University of Florida Research Foundation, Inc.

Inventor： Benjamin Earl VALLEY ,  Jason E BUTLER ,  Anthony J. LADD

IPC: B01L3/00

CPC classification number: B01L3/50273 ,  B01L3/502715 ,  B01L2300/16 ,  B01L2400/0421

Abstract: Provided herein are microfluidic devices that can be configured to generate an electrophoretic flow that is in opposition to a fluid flow through a microcapillary of a microfluidic device provided herein for nucleic acid isolation. Also provided herein are systems comprising such. Also provided herein are methods that include adding an amount of a sample comprising nucleic acids to the inlet area of a microfluidic device as provided herein, generating a first fluid flow through a microcapillary of a microfluidic device provided herein; and applying a uniform electric field to the microfluidic device, where the uniform electric field generates an electrophoretic flow that is in opposition to the fluid flow. Methods as described herein can further comprise quantifying or otherwise characterizing isolated nucleic acids separated by devices, systems, and methods as described herein.