公开(公告)号：US20220243171A1

公开(公告)日：2022-08-04

申请号：US17622102

申请日：2020-07-01

Applicant: University of Florida Research Foundation, Inc.

Inventor： Thomas Ettor ANGELINI ,  Cameron D MORLEY ,  Christopher S. O'BRYAN ,  Peter S. MCFETRIDGE ,  Malisa SARNTINORANONT ,  Tanmay P. LELE ,  Naohiro Terada

IPC: C12N5/00 ,  C12M1/12 ,  C12M1/00

Abstract: Described herein are systems and methods relating to three-dimensional (3D) cell manufacturing utilizing a 3D growth media. According to aspects of the present disclosure, pluralities of cells and ECM components can be printed into a bioreactor as a sheet. Sheets can be retrieved, digested, split, and re-printed, thereby expanding the number of cells in an efficient manner that conserves space in a tissue culture incubator, conserves bioreactor consumables, and consumes nutrients required for cell maintenance and growth.