公开(公告)号：US20190300861A1

公开(公告)日：2019-10-03

申请号：US16315675

申请日：2017-07-07

Applicant: University of Florida Research Foundation, Inc.

Inventor： CHRISTOPHER DILLON VULPE ,  AMIN SOBH ,  DAVID MICHAEL FAULKNER ,  ABDERRAHMANE TAGMOUNT ,  MICHAEL FASULLO

IPC: C12N5/16 ,  C12N15/10 ,  C12N15/113 ,  C12N9/06 ,  C12N9/10 ,  G01N33/50 ,  C12N15/90

Abstract: Provided herein are genetically engineered cells containing one or more modulated metabolic genes, where the expression of the modulated metabolic gene(s) can be greater than that of an unmodified control. Also provided herein are methods of making the genetically engineered cells using synergistic activation mediator CRISPR-Cas9. Further provided herein are high throughput assays that can employ the genetically engineered cells provided herein.