公开(公告)号：US20210163451A1

公开(公告)日：2021-06-03

申请号：US17049708

申请日：2019-05-16

Applicant: University of Florida Research Foundation, Inc.

Inventor： Yousong Ding ,  Guangde Jiang

IPC: C07D403/06 ,  A01N43/60

Abstract: Thaxtomins are phytotoxic secondary metabolites produced in plant pathogenic Streptomyces strains and have received considerable interests as bioherbicides. A cell-free, biocombinatorial approach was developed to produce a thaxtomin library for herbicide development. Combination of biosynthetic enzymes led to the production of 136 substituted 2,5-diketopiperazines, thaxtomin D, thaxtomin B and thaxtomin A analogs in a single pot. Furthermore, rational engineering of TxtA allowed the synthesis of azido-containing thaxtomin analog. Selected unnatural thaxtomins demonstrated improved herbicidal activities. This abstract is intended as a scanning tool for purposes of searching in the particular art and is not intended to be limiting of the present disclosure.

公开(公告)号：US11655477B2

公开(公告)日：2023-05-23

申请号：US16616059

申请日：2018-05-23

Applicant: University of Florida Research Foundation, Inc.

Inventor： Rosemary Loria ,  Yucheng Zhang ,  Yousong Ding ,  Guangde Jiang

IPC: C12N15/76 ,  C12N1/20 ,  C12P17/16 ,  A01N63/28

CPC classification number: C12N15/76 ,  A01N63/28 ,  C12N1/20 ,  C12P17/165 ,  C12N2510/02

Abstract: The present disclosure includes genetically engineered, non-pathogenic Streptomyces bacterium with exogenous, non-native Thaxtomin A (ThxA) biosynthetic gene clusters conferring the genetically engineered, non-pathogenic Streptomyces bacterium with the ability to produce thaxtomin A. Also included are methods of providing thaxtomin producing capability in non-native Streptomyces bacterial strains, methods of producing thaxtomin compounds with the genetically engineered Streptomyces bacteria of the present disclosure, and methods of producing thaxtomin compounds and nitro-tryptophan analogs, and fluorinated thaxtomin compounds, analogs, and intermediates with the genetically engineered Streptomyces bacteria of the present disclosure.

公开(公告)号：US11530415B2

公开(公告)日：2022-12-20

申请号：US16768347

申请日：2018-11-29

Applicant: University of Florida Research Foundation, Inc.

Inventor： Yousong Ding ,  Guangde Jiang

IPC: C12N15/76 ,  C12N1/20 ,  C12P17/16 ,  A01N63/28

Abstract: The present disclosure includes refactored thaxtomin biosynthetic gene clusters including thaxtomin modules including one or more thaxtomin genes such that the expression of the refactored thaxtomin biosynthetic gene cluster produces at least one thaxtomin compound in the absence of thaxtomin-inducing conditions. Also included are genetically engineered Streptomyces bacterium from a non-pathogenic Streptomyces strain comprising an exogenous, refactored thaxtomin biosynthetic gene cluster of the present disclosure, such that the expression of the refactored thaxtomin biosynthetic gene cluster provides the genetically engineered Streptomyces bacterium with the ability to produce at least one thaxtomin compound in the absence of thaxtomin-inducing conditions. The present disclosure also includes methods of producing thaxtomin compounds, analogs, or intermediate with the refactored thaxtomin biosynthetic gene clusters and genetically engineered bacteria of the present disclosure.