公开(公告)号：US20220213465A1

公开(公告)日：2022-07-07

申请号：US17526743

申请日：2021-11-15

申请人： University of Maryland, College Park ,  Canon U.S.A., Inc.

发明人： Ian M. White ,  Srinivasa Raghavan ,  Kunal R. Pandit ,  Imaly Nanayakkara ,  Weidong Cao

IPC分类号： C12N15/10 ,  C12Q1/686

摘要： The present invention relates to a method for concentrating a biological sample containing nucleic acids by using magnetic chitosan microparticles and subsequently performing a PCR reaction on the nucleic acids captured on the microparticles. The chitosan microparticles added to the biological sample at a PCR compatible pH are mechanically agitated to provide for cell lysis and simultaneous DNA capture, and then serve as a solid support for the nucleic acid template during the PCR reaction. As the chitosan microparticles are utilized for lysis and the nucleic acids do not need to be removed from the microparticles before PCR, the ease of the sample preparation procedure is dramatically improved.

公开(公告)号：US11174478B2

公开(公告)日：2021-11-16

申请号：US15160935

申请日：2016-05-20

申请人： University of Maryland ,  Canon U.S.A., Inc.

发明人： Ian M. White ,  Srinivasa Raghavan ,  Kunal R. Pandit ,  Imaly Nanayakkara ,  Weidong Cao

IPC分类号： C12Q1/68 ,  C12N15/10 ,  C12Q1/686 ,  B01L7/00 ,  B01L3/00

摘要： The present invention relates to a method for concentrating a biological sample containing nucleic acids by using magnetic chitosan microparticles and subsequently performing a PCR reaction on the nucleic acids captured on the microparticles. The chitosan microparticles added to the biological sample at a PCR compatible pH are mechanically agitated to provide for cell lysis and simultaneous DNA capture, and then serve as a solid support for the nucleic acid template during the PCR reaction. As the chitosan microparticles are utilized for lysis and the nucleic acids do not need to be removed from the microparticles before PCR, the ease of the sample preparation procedure is dramatically improved.