公开(公告)号：US20230183711A1

公开(公告)日：2023-06-15

申请号：US17767962

申请日：2020-10-14

Applicant: University of Massachusetts

Inventor： Guangping Gao ,  Dan Wang ,  Jote Bulcha

IPC: C12N15/52 ,  C07K14/005

CPC classification number: C12N15/52 ,  C07K14/005 ,  C12N2750/14122 ,  C12N2750/14143 ,  C12N2750/14152

Abstract: Aspects of the disclosure relate to manufacturing AAV using a stable cell line that expresses the rep gene by engineering tRNA to suppress a mutation introduced to a rep integrated into the genome of the cell line.