公开(公告)号：US11841492B2

公开(公告)日：2023-12-12

申请号：US17274625

申请日：2019-09-13

Applicant: University of Massachusetts

Inventor： David Grunwald ,  Maximiliaan Huisman ,  Karina Jouravleva ,  Carlas Smith ,  Phillip D. Zamore

IPC: G02B21/26 ,  G02B21/00 ,  G02B21/30 ,  G02B21/06

CPC classification number: G02B21/30 ,  G02B21/06 ,  G02B21/26

Abstract: The invention is directed to a heated stage assembly that can achieve high temperatures (i.e., 40 degrees Celsius and higher and more specifically, 55 degrees Celsius and higher) for use in microscopy including fluorescence microscopy. The microscope stage heater assembly includes a mounting adapter element, a sample holder element mounted within the mounting adapter element, one or more heating elements attached to the sample holder element, and an optical window holder element disposed on a surface of the sample holder element that encloses an optical window element. The optical window element may be in contact with a microscope sample such that the optical window element is heated by the sample holder element to uniformly distribute heat to the specimen.

公开(公告)号：US20220298507A1

公开(公告)日：2022-09-22

申请号：US17616750

申请日：2020-06-10

Applicant: Chan Zuckerberg Biohub, Inc. ,  The Board of Trustees of the Leland Stanford Junior University ,  University of Massachusetts

Inventor： William J. Greenleaf ,  Phillip D. Zamore ,  Winston R. Becker ,  Benjamin Ober-Reynolds ,  Karina Jouravleva ,  Samson M. Jolly

IPC: C12N15/113

Abstract: The disclosure provides inhibitory RNA polynucleotides that have partial complementarity to a target gene. The inhibitory RNA polynucleotides have at least one mismatched nucleotide and can be designed to increase or decrease the cleavage rate when loaded onto the RNA-induced silencing complex (RISC).