公开(公告)号：US11841492B2

公开(公告)日：2023-12-12

申请号：US17274625

申请日：2019-09-13

Applicant: University of Massachusetts

Inventor： David Grunwald ,  Maximiliaan Huisman ,  Karina Jouravleva ,  Carlas Smith ,  Phillip D. Zamore

IPC: G02B21/26 ,  G02B21/00 ,  G02B21/30 ,  G02B21/06

CPC classification number: G02B21/30 ,  G02B21/06 ,  G02B21/26

Abstract: The invention is directed to a heated stage assembly that can achieve high temperatures (i.e., 40 degrees Celsius and higher and more specifically, 55 degrees Celsius and higher) for use in microscopy including fluorescence microscopy. The microscope stage heater assembly includes a mounting adapter element, a sample holder element mounted within the mounting adapter element, one or more heating elements attached to the sample holder element, and an optical window holder element disposed on a surface of the sample holder element that encloses an optical window element. The optical window element may be in contact with a microscope sample such that the optical window element is heated by the sample holder element to uniformly distribute heat to the specimen.

公开(公告)号：US20200174353A1

公开(公告)日：2020-06-04

申请号：US16703614

申请日：2019-12-04

Applicant: University of Massachusetts

Inventor： David Grunwald ,  Maximiliaan Huisman ,  Carlas Smith

IPC: G03B21/20 ,  G02B21/00 ,  G02B21/36

Abstract: The described embodiments are directed to a system and methods of calibrating a fluorescence microscope and/or light detection device using a calibrating apparatus. The apparatus may comprise a main body housing, a sensor head, and a microcontroller assembly disposed within the housing. The housing may include an adapter to mechanically couple the housing to a microscope. The sensor head may comprise (i) an optical power sensor to produce a power signal representative of an optical power magnitude of light applied to the optical power sensor, (ii) an optical wavelength sensor configured to produce wavelength information associated with the light applied to the optical wavelength sensor, and (iii) a light source configured to direct light toward a detection device associated with the microscope. The microcontroller assembly may be configured to generate an optical power magnitude value based on the power signal and adjusted according to the wavelength information.

公开(公告)号：US10890838B2

公开(公告)日：2021-01-12

申请号：US16703614

申请日：2019-12-04

Applicant: University of Massachusetts

Inventor： David Grunwald ,  Maximiliaan Huisman ,  Carlas Smith

IPC: G03B21/20 ,  G03B21/00 ,  G02B21/00 ,  G02B21/36

Abstract: The described embodiments are directed to a system and methods of calibrating a fluorescence microscope and/or light detection device using a calibrating apparatus. The apparatus may comprise a main body housing, a sensor head, and a microcontroller assembly disposed within the housing. The housing may include an adapter to mechanically couple the housing to a microscope. The sensor head may comprise (i) an optical power sensor to produce a power signal representative of an optical power magnitude of light applied to the optical power sensor, (ii) an optical wavelength sensor configured to produce wavelength information associated with the light applied to the optical wavelength sensor, and (iii) a light source configured to direct light toward a detection device associated with the microscope. The microcontroller assembly may be configured to generate an optical power magnitude value based on the power signal and adjusted according to the wavelength information.

公开(公告)号：US20210041778A1

公开(公告)日：2021-02-11

申请号：US17069335

申请日：2020-10-13

Applicant: University of Massachusetts

Inventor： David Grunwald ,  Maximiliaan Huisman ,  Carlas Smith

IPC: G03B21/20 ,  G02B21/00

Abstract: The described embodiments are directed to a system and methods of calibrating a fluorescence microscope and/or light detection device using a calibrating apparatus. The apparatus may comprise a main body housing, a sensor head, and a microcontroller assembly disposed within the housing. The housing may include an adapter to mechanically couple the housing to a microscope. The sensor head may comprise (i) an optical power sensor to produce a power signal representative of an optical power magnitude of light applied to the optical power sensor, (ii) an optical wavelength sensor configured to produce wavelength information associated with the light applied to the optical wavelength sensor, and (iii) a light source configured to direct light toward a detection device associated with the microscope. The microcontroller assembly may be configured to generate an optical power magnitude value based on the power signal and adjusted according to the wavelength information.