公开(公告)号：US20230022632A1

公开(公告)日：2023-01-26

申请号：US17812494

申请日：2022-07-14

申请人： University of Rochester

发明人： Qiang Yang ,  Jennifer Hunter ,  Keith Parkins

IPC分类号： A61B3/13 ,  A61B3/12 ,  G02B21/16 ,  G02B21/00

摘要： A microscopy imaging system comprises a fluorescence lifetime imaging microscopy (FLIM) system comprising a pulsed light source configured to direct a plurality of excitation light pulses onto a sample, a photo detector configured to detect emitted fluorescent photons created by the plurality of excitation pulses interacting with the sample, and a FLIM data acquisition system configured to measure the time interval between the excitation light pulses and the detected emitted fluorescent photons, a scanning light microscopy (SLM) system comprising a SLM data acquisition system, a fast scanning mirror and a slow scanning mirror, wherein the mirrors are configured to scan the light pulses across the sample; and a data processing system communicatively connected to the FLIM and SLM systems. Microscopy imaging methods are also disclosed.