公开(公告)号：US12085533B2

公开(公告)日：2024-09-10

申请号：US18475534

申请日：2023-09-27

Applicant: University of Washington

Inventor： Jens H. Gundlach ,  Ian Michael Derrington ,  Andrew Laszlo ,  Jonathan Craig ,  Henry Brinkerhoff

IPC: G01N27/447 ,  G01N33/487 ,  G01N33/68

CPC classification number: G01N27/44743 ,  G01N27/44791 ,  G01N33/48721 ,  G01N33/6818

Abstract: Methods for nanopore-based protein analysis are provided. The methods address the characterization of a target protein analyte, which has a dimension greater than an internal diameter of the nanopore tunnel, and which is also physically associated with a polymer. The methods further comprise applying an electrical potential to the nanopore system to cause the polymer to interact with the nanopore tunnel. The ion current through the nanopore is measured to provide a current pattern reflective of the structure of the portion of the polymer interacting with the nanopore tunnel. This is used as a metric for characterizing the associated protein that does not pass through the nanopore.

公开(公告)号：US10948454B2

公开(公告)日：2021-03-16

申请号：US16517996

申请日：2019-07-22

Applicant: University of Washington

Inventor： Jens Gundlach ,  Ian Michael Derrington ,  Andrew Laszlo ,  Jonathan Craig ,  Henry Brinkerhoff

IPC: G01N27/447 ,  G01N33/487 ,  G01N33/68

Abstract: Methods for nanopore-based protein analysis are provided. The methods address the characterization of a target protein analyte, which has a dimension greater than an internal diameter of the nanopore tunnel, and which is also physically associated with a polymer. The methods further comprise applying an electrical potential to the nanopore system to cause the polymer to interact with the nanopore tunnel. The ion current through the nanopore is measured to provide a current pattern reflective of the structure of the portion of the polymer interacting with the nanopore tunnel. This is used as a metric for characterizing the associated protein that does not pass through the nanopore.

公开(公告)号：US20240377357A1

公开(公告)日：2024-11-14

申请号：US18789966

申请日：2024-07-31

Applicant: University of Washington

Inventor： Jens H. Gundlach ,  Ian Michael Derrington ,  Andrew Laszlo ,  Jonathan Craig ,  Henry Brinkerhoff

IPC: G01N27/447 ,  G01N33/487 ,  G01N33/68

Abstract: Methods for nanopore-based protein analysis are provided. The methods address the characterization of a target protein analyte, which has a dimension greater than an internal diameter of the nanopore tunnel, and which is also physically associated with a polymer. The methods further comprise applying an electrical potential to the nanopore system to cause the polymer to interact with the nanopore tunnel. The ion current through the nanopore is measured to provide a current pattern reflective of the structure of the portion of the polymer interacting with the nanopore tunnel. This is used as a metric for characterizing the associated protein that does not pass through the nanopore.

公开(公告)号：US10359395B2

公开(公告)日：2019-07-23

申请号：US15120286

申请日：2014-11-26

Applicant: University of Washington

Inventor： Jens Gundlach ,  Ian Michael Derrington ,  Andrew Laszlo ,  Jonathan Craig ,  Henry Brinkerhoff

IPC: G01N27/447 ,  G01N33/487 ,  G01N33/68

Abstract: Methods for nanopore-based protein analysis are provided. The methods address the characterization of a target protein analyte, which has a dimension greater than an internal diameter of the nanopore tunnel, and which is also physically associated with a polymer. The methods further comprise applying an electrical potential to the nanopore system to cause the polymer to interact with the nanopore tunnel. The ion current through the nanopore is measured to provide a current pattern reflective of the structure of the portion of the polymer interacting with the nanopore tunnel. This is used as a metric for characterizing the associated protein that does not pass through the nanopore.

公开(公告)号：US20240085372A1

公开(公告)日：2024-03-14

申请号：US18475534

申请日：2023-09-27

Applicant: University of Washington

Inventor： Jens H. Gundlach ,  Ian Michael Derrington ,  Andrew Laszlo ,  Jonathan Craig ,  Henry Brinkerhoff

IPC: G01N27/447 ,  G01N33/487 ,  G01N33/68

CPC classification number: G01N27/44743 ,  G01N27/44791 ,  G01N33/48721 ,  G01N33/6818

Abstract: Methods for nanopore-based protein analysis are provided. The methods address the characterization of a target protein analyte, which has a dimension greater than an internal diameter of the nanopore tunnel, and which is also physically associated with a polymer. The methods further comprise applying an electrical potential to the nanopore system to cause the polymer to interact with the nanopore tunnel. The ion current through the nanopore is measured to provide a current pattern reflective of the structure of the portion of the polymer interacting with the nanopore tunnel. This is used as a metric for characterizing the associated protein that does not pass through the nanopore.

公开(公告)号：US11808734B2

公开(公告)日：2023-11-07

申请号：US17202118

申请日：2021-03-15

Applicant: University of Washington

Inventor： Jens Gundlach ,  Ian Michael Derrington ,  Andrew Laszlo ,  Jonathan Craig ,  Henry Brinkerhoff

IPC: G01N27/447 ,  G01N33/487 ,  G01N33/68

CPC classification number: G01N27/44743 ,  G01N27/44791 ,  G01N33/48721 ,  G01N33/6818

Abstract: Methods for nanopore-based protein analysis are provided. The methods address the characterization of a target protein analyte, which has a dimension greater than an internal diameter of the nanopore tunnel, and which is also physically associated with a polymer. The methods further comprise applying an electrical potential to the nanopore system to cause the polymer to interact with the nanopore tunnel. The ion current through the nanopore is measured to provide a current pattern reflective of the structure of the portion of the polymer interacting with the nanopore tunnel. This is used as a metric for characterizing the associated protein that does not pass through the nanopore.

公开(公告)号：US20210293748A1

公开(公告)日：2021-09-23

申请号：US17202118

申请日：2021-03-15

Applicant: University of Washington

Inventor： Jens Gundlach ,  Ian Michael Derrington ,  Andrew Laszlo ,  Jonathan Craig ,  Henry Brinkerhoff

IPC: G01N27/447 ,  G01N33/487 ,  G01N33/68

Abstract: Methods for nanopore-based protein analysis are provided. The methods address the characterization of a target protein analyte, which has a dimension greater than an internal diameter of the nanopore tunnel, and which is also physically associated with a polymer. The methods further comprise applying an electrical potential to the nanopore system to cause the polymer to interact with the nanopore tunnel. The ion current through the nanopore is measured to provide a current pattern reflective of the structure of the portion of the polymer interacting with the nanopore tunnel. This is used as a metric for characterizing the associated protein that does not pass through the nanopore.

公开(公告)号：US20200049656A1

公开(公告)日：2020-02-13

申请号：US16517996

申请日：2019-07-22

Applicant: University of Washington

Inventor： Jens Gundlach ,  Ian Michael Derrington ,  Andrew Laszlo ,  Jonathan Craig ,  Henry Brinkerhoff

IPC: G01N27/447 ,  G01N33/487 ,  G01N33/68

Abstract: Methods for nanopore-based protein analysis are provided. The methods address the characterization of a target protein analyte, which has a dimension greater than an internal diameter of the nanopore tunnel, and which is also physically associated with a polymer. The methods further comprise applying an electrical potential to the nanopore system to cause the polymer to interact with the nanopore tunnel. The ion current through the nanopore is measured to provide a current pattern reflective of the structure of the portion of the polymer interacting with the nanopore tunnel. This is used as a metric for characterizing the associated protein that does not pass through the nanopore.

公开(公告)号：US20170199149A1

公开(公告)日：2017-07-13

申请号：US15120286

申请日：2014-11-26

Applicant: University of Washington

Inventor： Jens Gundlach ,  Ian Michael Derrington ,  Andrew Laszlo ,  Jonathan Craig ,  Henry Brinkerhoff

IPC: G01N27/447 ,  G01N33/68 ,  G01N33/487

CPC classification number: G01N27/44743 ,  G01N27/44791 ,  G01N33/48721 ,  G01N33/6818

Abstract: Methods for nanopore-based protein analysis are provided. The methods address the characterization of a target protein analyte, which has a dimension greater than an internal diameter of the nanopore tunnel, and which is also physically associated with a polymer. The methods further comprise applying an electrical potential to the nanopore system to cause the polymer to interact with the nanopore tunnel. The ion current through the nanopore is measured to provide a current pattern reflective of the structure of the portion of the polymer interacting with the nanopore tunnel. This is used as a metric for characterizing the associated protein that does not pass through the nanopore.