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公开(公告)号:US20050014142A1
公开(公告)日:2005-01-20
申请号:US10479866
申请日:2002-06-06
申请人: Uwe Scherf , Michael Elashoff , Yasmin Beazer-Barclay , Kristen Antonellis , Scott Jelinsky , Maryann Whitley , Eugene Brown
发明人: Uwe Scherf , Michael Elashoff , Yasmin Beazer-Barclay , Kristen Antonellis , Scott Jelinsky , Maryann Whitley , Eugene Brown
IPC分类号: C12Q1/68 , C12Q1/6832 , C12Q1/6837
CPC分类号: C12Q1/6832 , C12Q1/6837 , C12Q2545/113
摘要: The present invention includes methods of normalizing hybridization reactions that are designed to select normalization control genes, specifically 5′-3′-, and middle portions of the these genes, that hybridize similarly to a probe array and that produce the most consistently linear curve of hybridization signal over a range of normalization control gene segment concentrations. These methods have applicability across a broad spectrum of hybridization formats.
摘要翻译: 本发明包括归一化杂交反应的方法,其被设计用于选择归一化对照基因,特别是这些基因的5'-3'-和中间部分,其与探针阵列类似地杂交并产生最一致的线性曲线 杂交信号在一系列归一化控制基因片段浓度。 这些方法在广泛的杂交格式中具有适用性。