公开(公告)号：US09977017B2

公开(公告)日：2018-05-22

申请号：US15285200

申请日：2016-10-04

申请人： VALNEVA ,  TOYAMA PREFECTURE

发明人： Aishun Jin ,  Hiroyuki Kishi ,  Atsushi Muraguchi ,  Tsutomu Obata

IPC分类号： G01N33/543 ,  G01N33/50 ,  G01N33/566 ,  G01N33/68

CPC分类号： G01N33/54366 ,  G01N33/5047 ,  G01N33/566 ,  G01N33/6863 ,  G01N2333/52 ,  G01N2333/715

摘要： Provided are a method and means permitting the simultaneous measurement of the reactive properties of more than 10,000 of antigen-stimulated lymphocytes being held on a chip and the separate determination of the states of individual cells. A microwell array comprises multiple wells and a coating layer on one of the principal surfaces of a base member, the wells being of a size permitting the entry of only a single cell into each well. A coating layer of a substance capable of binding to a substance produced by the cells contained in the wells is present on the principal surface around the wells. A method of screening for a target cell, comprises: causing specimen cells and a cell culture broth to be contained in the wells of the above microwell array; immersing the coating layer and the wells in the culture broth and culturing the cells in a state permitting the diffusion of substances in the culture broth from the wells into the coating layer; feeding a label substance binding specifically to a substance produced by a target cell present among the specimen cells onto the coating layer; and detecting the substance produced by the target cell that has bound to the substance in the coating layer by the label substance to specify the target cell.