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公开(公告)号：US20170023562A1
公开(公告)日：2017-01-26
申请号：US15285200
申请日：2016-10-04
申请人： VALNEVA , TOYAMA PREFECTURE
发明人： Aishun JIN , Hiroyuki KISHI , Atsushi MURAGUCHI , Tsutomu OBATA
IPC分类号： G01N33/543 , G01N33/566 , G01N33/68
CPC分类号： G01N33/54366 , G01N33/5047 , G01N33/566 , G01N33/6863 , G01N2333/52 , G01N2333/715
摘要： Provided are a method and means permitting the simultaneous measurement of the reactive properties of more than 10,000 of antigen-stimulated lymphocytes being held on a chip and the separate determination of the states of individual cells. A microwell array comprises multiple wells and a coating layer on one of the principal surfaces of a base member, the wells being of a size permitting the entry of only a single cell into each well. A coating layer of a substance capable of binding to a substance produced by the cells contained in the wells is present on the principal surface around the wells. A method of screening for a target cell, comprises: causing specimen cells and a cell culture broth to be contained in the wells of the above microwell array; immersing the coating layer and the wells in the culture broth and culturing the cells in a state permitting the diffusion of substances in the culture broth from the wells into the coating layer; feeding a label substance binding specifically to a substance produced by a target cell present among the specimen cells onto the coating layer; and detecting the substance produced by the target cell that has bound to the substance in the coating layer by the label substance to specify the target cell.
摘要翻译： 提供了允许同时测量保持在芯片上的超过10,000个抗原刺激的淋巴细胞的反应性的方法和手段，以及单独测定各细胞的状态。 微孔阵列包括多个孔和在基底构件的一个主表面上的涂层，所述孔具有允许仅将单个细胞进入每个孔的尺寸。 能够与孔中包含的细胞产生的物质结合的物质的涂层存在于孔周围的主表面上。 筛选靶细胞的方法包括：使样品细胞和细胞培养液包含在上述微孔阵列的孔中; 将涂层和孔浸入培养液中，并使培养液中的物质从孔扩散到涂层中的状态下培养细胞; 将标本物质特异性地与存在于试样细胞中的靶细胞产生的物质结合到涂层上; 以及通过所述标记物质检测由所述靶层细胞产生的与所述涂层中的物质结合的物质，以指定所述靶细胞。
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公开(公告)号：US20210039103A1
公开(公告)日：2021-02-11
申请号：US16978161
申请日：2019-03-08
发明人： Kouji TAKATA , Shingi HASHIOKA
摘要： A particle separation device include a syringe, a barrel into which a sample liquid S containing target particles is injected and a barrel into which a buffer liquid B is injected. The device also includes a branching tube including a first conduit connected to a discharge port of the syringe, a second conduit corresponding to one of two branching from the first conduit and connected to an injection port of the barrel, and a third conduit corresponding to the other of two branching from the first conduit and connected to an injection port of the barrel. The device further includes a first unidirectional valve provided in the middle of the first conduit so as to prevent a backflow and operated to be opened in a positive pressure state on the side of the syringe and to be closed in a negative pressure state on the side of the syringe.
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公开(公告)号：US20170203471A1
公开(公告)日：2017-07-20
申请号：US15476446
申请日：2017-03-31
发明人： Tsutomu OBATA , Yoshiyuki YOKOYAMA , Masaaki NASUNO , Mikiharu KUCHIKI , Tomoshi AONO , Motofumi KASHIWAGI
CPC分类号： B29C33/424 , B29C33/38 , B29C33/3842 , B29C59/002 , B29C2033/426 , B29K2845/00 , B29K2995/0026 , G03F7/0002
摘要： An imprint template is used for photocuring imprinting, and includes a support plate that is transparent with respect to an exposure wavelength used for photocuring imprinting, and has flexibility; a buffer resin layer that is formed on the support plate, and is formed of an elastic material that is transparent with respect to the exposure wavelength; and a resin film mold that is removably bonded to the buffer resin layer, and is transparent with respect to the exposure wavelength, wherein a concave-convex transfer pattern is formed on a surface of the resin film mold.
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公开(公告)号：US20230007988A1
公开(公告)日：2023-01-12
申请号：US17950319
申请日：2022-09-22
申请人： TOYAMA PREFECTURE
发明人： Hideki YAMAGISHI
IPC分类号： B23K20/02 , B23K20/227
摘要： The method comprises applying a spot load to a joint part between a first metal material and a second metal material in a state where sites to form the joint part are superposed on each other. When a total thickness of the first metal material and the second metal material at the joint part before bonding is defined as T0 mm, the total thickness thereof after bonding is defined as T1 mm, and T0/T1=R is defined as a reduction ratio, the reduction ratio R is 1.4 or more.
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公开(公告)号：US20220146934A1
公开(公告)日：2022-05-12
申请号：US17429489
申请日：2020-01-16
摘要： A method for producing a metal pattern by processing a substrate having on its surface a metal layer with a photosensitive fiber having a specific composition, a method for producing a metal pattern, and a composition for producing the photosensitive fiber. The photosensitive fiber contains a positive photosensitive material. The positive photosensitive material may contain a novolac resin, etc. The method for producing a metal pattern includes a first step of forming a fiber layer of photosensitive resin on a substrate having on its surface a metal layer; a second step of exposing the fiber layer to light via a mask; a third step of developing the fiber layer with a developer to thereby form a photosensitive fiber pattern; and a fourth step of etching the metal layer with an etchant and removing the photosensitive fiber, to thereby form a network metal pattern.
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公开(公告)号：US20180148859A1
公开(公告)日：2018-05-31
申请号：US15568482
申请日：2016-04-22
CPC分类号： D01D5/003 , D01D5/04 , D01F1/10 , D01F6/50 , G03F7/022 , G03F7/038 , G03F7/0382 , G03F7/039 , G03F7/0392
摘要： The invention provides a method capable of conveniently producing an intricate and fine resist pattern. The invention also provides a fiber containing a positive-type or negative-type photosensitive material.
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公开(公告)号：US09695460B2
公开(公告)日：2017-07-04
申请号：US14016408
申请日：2013-09-03
发明人： Yasuhisa Asano , Masafumi Kameya , Hiroyasu Onaka
IPC分类号： C12Q1/26
CPC分类号： C12Q1/26 , C12Y104/03
摘要： Disclosed is a method for quantifying L-tryptophan involving a step for mixing a specimen, L-tryptophan oxidase, and water, a step for allowing the obtained reaction solution to stand a predetermined period of time in the presence of oxygen, and a step for measuring the reaction product resulting from action of enzymes present in the reaction solution after allowing to stand. Also disclosed are a kit used to quantify the L-tryptophan containing L-tryptophan oxidase, and an enzyme sensor using the L-tryptophan oxidase. This method, kit and enzyme sensor use an L-tryptophan-specific enzyme, so are capable of quantifying L-tryptophan even in the presence of other amino acids.
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8.发明申请Method of Analyzing L-Tryptophan in Biological Samples, and Kit Used Therein 有权生物样品中L-色氨酸的分析方法及其中使用的试剂盒公开(公告)号：US20130344526A1
公开(公告)日：2013-12-26
申请号：US14016408
申请日：2013-09-03
发明人： Yasuhisa Asano , Masafumi Kameya , Hiroyasu Onaka
IPC分类号： C12Q1/26
CPC分类号： C12Q1/26 , C12Y104/03
摘要： Disclosed is a method for quantifying L-tryptophan involving a step for mixing a specimen, L-tryptophan oxidase, and water, a step for allowing the obtained reaction solution to stand a predetermined period of time in the presence of oxygen, and a step for measuring the reaction product resulting from action of enzymes present in the reaction solution after allowing to stand. The L-tryptophan oxidase has a given amino acid sequence and has oxidase activity that generates hydrogen peroxide and ammonia by acting on the L-tryptophan in the presence of oxygen and water. The oxidase activity of the L-tryptophan oxidase on the L-phenylalanine is in the range of 0-3% of the oxidase activity thereof on the L-tryptophan, and the L-tryptophan oxidase does not have oxidase activity on protein-constituting amino acids other than L-tryptophan and L-phenylalanine. Also disclosed are a kit used to quantify the L-tryptophan containing L-tryptophan oxidase, and an enzyme sensor using the L-tryptophan oxidase. This method, kit and enzyme sensor use an L-tryptophan-specific enzyme, so are capable of quantifying L-tryptophan even in the presence of other amino acids.
摘要翻译： 本发明公开了一种定量L-色氨酸的方法，其涉及将样品，L-色氨酸氧化酶和水混合的步骤，使得所得反应溶液在氧气存在下放置预定时间的步骤，以及用于 测量在静置后在反应溶液中存在的酶的作用产生的反应产物。 L-色氨酸氧化酶具有给定的氨基酸序列，具有通过在氧和水存在下作用于L-色氨酸而产生过氧化氢和氨的氧化酶活性。 L-色氨酸氧化酶对L-苯丙氨酸的氧化酶活性在L-色氨酸上的氧化酶活性的0-3％的范围内，并且L-色氨酸氧化酶对构成蛋白质的氨基没有氧化酶活性 L-色氨酸和L-苯丙氨酸以外的酸。 还公开了用于定量含有L-色氨酸的L-色氨酸氧化酶的试剂盒，以及使用L-色氨酸氧化酶的酶传感器。 这种方法，试剂盒和酶传感器使用L-色氨酸特异性酶，因此即使在其他氨基酸存在下也能够定量L-色氨酸。
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公开(公告)号：US10222697B2
公开(公告)日：2019-03-05
申请号：US15030049
申请日：2014-10-17
IPC分类号： D01F6/14 , G03F7/09 , D01D5/00 , D01F1/10 , D01F2/28 , D01F6/26 , C08F220/56 , C08L3/02 , G03F7/038
摘要： The invention provides a fiber containing (A) a polymer compound containing a structural unit having, in a side chain, at least one kind of organic group selected from a hydroxy group, a hydroxymethyl group and an alkoxymethyl group having 1-5 carbon atoms, and (B) a photoacid generator.
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公开(公告)号：US09977017B2
公开(公告)日：2018-05-22
申请号：US15285200
申请日：2016-10-04
申请人： VALNEVA , TOYAMA PREFECTURE
发明人： Aishun Jin , Hiroyuki Kishi , Atsushi Muraguchi , Tsutomu Obata
IPC分类号： G01N33/543 , G01N33/50 , G01N33/566 , G01N33/68
CPC分类号： G01N33/54366 , G01N33/5047 , G01N33/566 , G01N33/6863 , G01N2333/52 , G01N2333/715
摘要： Provided are a method and means permitting the simultaneous measurement of the reactive properties of more than 10,000 of antigen-stimulated lymphocytes being held on a chip and the separate determination of the states of individual cells. A microwell array comprises multiple wells and a coating layer on one of the principal surfaces of a base member, the wells being of a size permitting the entry of only a single cell into each well. A coating layer of a substance capable of binding to a substance produced by the cells contained in the wells is present on the principal surface around the wells. A method of screening for a target cell, comprises: causing specimen cells and a cell culture broth to be contained in the wells of the above microwell array; immersing the coating layer and the wells in the culture broth and culturing the cells in a state permitting the diffusion of substances in the culture broth from the wells into the coating layer; feeding a label substance binding specifically to a substance produced by a target cell present among the specimen cells onto the coating layer; and detecting the substance produced by the target cell that has bound to the substance in the coating layer by the label substance to specify the target cell.
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