公开(公告)号：US11359185B2

公开(公告)日：2022-06-14

申请号：US15064792

申请日：2016-03-09

申请人： Ventana Medical Systems, Inc.

发明人： Christopher Bieniarz ,  Jerome W Kosmeder ,  Mark Lefever ,  Casey A Kernag ,  Julia Ashworth-Sharpe ,  Jennifer Wong

IPC分类号： G01N33/543 ,  G01N33/552 ,  C12N9/16 ,  A61K47/68 ,  A61K47/65 ,  G01N33/535 ,  C07K16/42 ,  C07K16/44 ,  C12N9/08 ,  C12N9/96 ,  G01N33/53

摘要： Antibody/signal-generating moiety conjugates are disclosed that include an antibody covalently linked to a signal-generating moiety through a heterobifunctional polyalkyleneglycol linker. The disclosed conjugates show exceptional signal-generation in immunohistochemical and in situ hybridization assays on tissue sections and cytology samples. In one embodiment, enzyme-metallographic detection of nucleic acid sequences with hapten-labeled probes can be accomplished using the disclosed conjugates as a primary antibody without amplification.