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1.发明公开公开(公告)号：US20240264050A1
公开(公告)日：2024-08-08
申请号：US18640578
申请日：2024-04-19
CPC分类号： G01N1/30 , C12Q1/04 , G01N1/312 , G01N2001/302
摘要： Provided herein are methods of staining biological material for the purpose of detecting, and in some examples also identifying, microorganisms. Methods of Gram staining bacteria using a slow-acting decolorizing formulation, such as one that includes 1,2-propandiol or ethylene glycol, can be used to extend the time of the decolorizing step, and thus permit automation of the Gram staining method. Also provided are compositions and kits for performing automated Gram staining on microscope slides.
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2.发明申请GRAM STAINING METHOD WITH IMPROVED DECOLORIZATION OF THE CRYSTAL VIOLET-IODINE COMPLEX FROM GRAM NEGATIVE BACTERIA 审中-公开GRAM染色方法具有改善灰色阴性细菌的晶状体 - 碘复合物的脱色作用公开(公告)号：US20150211965A1
公开(公告)日：2015-07-30
申请号：US14680820
申请日：2015-04-07
发明人： Lesley Schumack , Parula Mehta , Setareh Duquette
IPC分类号： G01N1/31
CPC分类号： G01N1/30 , C12Q1/04 , G01N1/312 , G01N2001/302
摘要： Provided herein are methods of staining biological material for the purpose of detecting, and in some examples also identifying, microorganisms. Methods of Gram staining bacteria using a slow-acting decolorizing formulation, such as one that includes 1,2-propandiol or ethylene glycol, can be used to extend the time of the decolorizing step, and thus permit automation of the Gram staining method. Also provided are compositions and kits for performing automated Gram staining on microscope slides.
摘要翻译： 本文提供了为了检测和在一些实例中还鉴定微生物的方法来染色生物材料。 可以使用使用缓慢作用的脱色制剂（例如包括1,2-丙二醇或乙二醇）的革兰氏染色细菌的方法延长脱色步骤的时间，从而允许革兰氏染色法的自动化。 还提供了用于在显微镜载片上进行自动革兰氏染色的组合物和试剂盒。
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3.发明申请公开(公告)号：US20160091401A1
公开(公告)日：2016-03-31
申请号：US14938307
申请日：2015-11-11
CPC分类号： G01N1/30 , C12Q1/04 , G01N1/312 , G01N2001/302
摘要： Provided herein are methods of staining biological material for the purpose of detecting, and in some examples also identifying, microorganisms. Methods of Gram staining bacteria using a slow-acting decolorizing formulation, such as one that includes 1,2-propandiol or ethylene glycol, can be used to extend the time of the decolorizing step, and thus permit automation of the Gram staining method. Also provided are compositions and kits for performing automated Gram staining on microscope slides.
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4.发明授权Gram staining method with improved decolorization of the crystal violet-iodine complex from gram negative bacteria 有权革兰氏染色法，改善革兰氏阴性细菌结晶紫 - 碘络合物的脱色公开(公告)号：US09023615B2
公开(公告)日：2015-05-05
申请号：US13773864
申请日：2013-02-22
CPC分类号： G01N1/30 , C12Q1/04 , G01N1/312 , G01N2001/302
摘要： Provided herein are methods of staining biological material for the purpose of detecting, and in some examples also identifying, microorganisms. Methods of Gram staining bacteria using a slow-acting decolorizing formulation, such as one that includes 1,2-propandiol or ethylene glycol, can be used to extend the time of the decolorizing step, and thus permit automation of the Gram staining method. Also provided are compositions and kits for performing automated Gram staining on microscope slides.
摘要翻译： 本文提供了为了检测和在一些实例中还鉴定微生物的方法来染色生物材料。 可以使用使用缓慢作用的脱色制剂（例如包括1,2-丙二醇或乙二醇）的革兰氏染色细菌的方法延长脱色步骤的时间，从而允许革兰氏染色法的自动化。 还提供了用于在显微镜载片上进行自动革兰氏染色的组合物和试剂盒。
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