公开(公告)号：US20040132683A1

公开(公告)日：2004-07-08

申请号：US10732416

申请日：2003-12-11

申请人： Vical Incorporated

发明人： Philip L. Felgner ,  Jon Asher Wolff ,  Gary H. Rhodes ,  Robert Wallace Malone ,  Dennis A. Carson

IPC分类号： A61K048/00

CPC分类号： C07K14/005 ,  A61K9/1272 ,  A61K48/00 ,  C07K14/61 ,  C12N9/0069 ,  C12N9/1033 ,  C12N9/1247 ,  C12N9/78 ,  C12N15/87 ,  C12N2740/16122 ,  C12Y113/12007

摘要： A method for delivering a naked or isolated polynucleotide to the interior of a cell in a vertebrate, comprising the interstitial introduction of a naked polynucleotide into a tissue of the vertebrate where the polynucleotide is taken up by the cells of the tissue and exerts a therapeutic effect on the vertebrate. The method can be used to deliver a therapeutic polypeptide to the cells of the vertebrate, to provide an immune response upon in vivo translation of the polynucleotide, to deliver antisense polynucleotides, to deliver receptors to the cells of the vertebrate, or to provide transitory gene therapy.

摘要翻译： 一种用于将裸露或分离的多核苷酸递送至脊椎动物细胞内部的方法，包括将裸多核苷酸间插入脊椎动物的组织中，其中所述多核苷酸被组织的细胞吸收并发挥治疗效果 在脊椎动物上。 该方法可用于将治疗性多肽递送至脊椎动物的细胞，以在多核苷酸的体内翻译中提供免疫应答，递送反义多核苷酸，将受体递送至脊椎动物的细胞，或提供短暂基因 治疗。

公开(公告)号：US20040023911A1

公开(公告)日：2004-02-05

申请号：US10387525

申请日：2003-03-14

申请人： Vical Incorporated ,  Wisconsin Alumni Research Foundation

发明人： Philip L. Felgner ,  Jon Asher Wolff ,  Gary H. Rhodes ,  Robert Wallace Malone ,  Dennis A. Carson

IPC分类号： A61K048/00

CPC分类号： C07K14/005 ,  A61K9/1272 ,  A61K48/00 ,  C07K14/61 ,  C12N9/0069 ,  C12N9/1033 ,  C12N9/1247 ,  C12N9/78 ,  C12N15/87 ,  C12N2740/16122 ,  C12Y113/12007

摘要： A method for delivering a naked or isolated polynucleotide to the interior of a cell in a vertebrate, comprising the interstitial introduction of a naked polynucleotide into a tissue of the vertebrate where the polynucleotide is taken up by the cells of the tissue and exerts a therapeutic effect on the vertebrate. The method can be used to deliver a therapeutic polypeptide to the cells of the vertebrate, to provide an immune response upon in vivo translation of the polynucleotide, to deliver antisense polynucleotides, to deliver receptors to the cells of the vertebrate, or to provide transitory gene therapy.

公开(公告)号：US20030032615A1

公开(公告)日：2003-02-13

申请号：US10202858

申请日：2002-07-26

申请人： Vical Incorporated

发明人： Philip L. Felgner ,  Jon Asher Wolff ,  Gary H. Rhodes ,  Robert Wallace Malone ,  Dennis A. Carson

IPC分类号： A61K048/00

CPC分类号： C07K14/005 ,  A61K9/1272 ,  A61K48/00 ,  C07K14/61 ,  C12N9/0069 ,  C12N9/1033 ,  C12N9/1247 ,  C12N9/78 ,  C12N15/87 ,  C12N2740/16122 ,  C12Y113/12007

摘要： A method for delivering an isolated polynucleotide to the interior of a cell in a vertebrate, comprising the interstitial introduction of an isolated polynucleotide into a tissue of the vertebrate where the polynucleotide is taken up by the cells of the tissue and exerts a therapeutic effect on the vertebrate. The method can be used to deliver a therapeutic polypeptide to the cells of the vertebrate, to provide an immune response upon in vivo translation of the polynucleotide, to deliver antisense polynucleotides, to deliver receptors to the cells of the vertebrate, or to provide transitory gene therapy.

摘要翻译： 一种用于将分离的多核苷酸递送至脊椎动物细胞内部的方法，包括将分离的多核苷酸间插入脊椎动物的组织中，其中所述多核苷酸被组织的细胞吸收并对其施用治疗效果 脊椎动物。 该方法可用于将治疗性多肽递送至脊椎动物的细胞，以在多核苷酸的体内翻译中提供免疫应答，递送反义多核苷酸，将受体递送至脊椎动物的细胞，或提供短暂基因 治疗。