公开(公告)号：US08993304B2

公开(公告)日：2015-03-31

申请号：US13855090

申请日：2013-04-02

申请人： Novozymes A/S

发明人： Steen Troels Jorgensen

IPC分类号： C12N1/20 ,  C12N9/10 ,  C12N15/00 ,  C12N15/74 ,  C12P21/06 ,  C07H21/04 ,  C12N15/75

CPC分类号： C12N15/75 ,  C12N9/1033

摘要： The present invention relates to a modified Bacillus licheniformis host cell, wherein one or more naturally occurring chromosomal chloramphenicol acetyl transferase encoding gene(s), cat, has been inactivated. The inactivation of the chromosomal cat gene(s) allows the use of chloramphenicol as an efficient selective agent in methods for DNA introduction into the modified cell.

摘要翻译： 本发明涉及一种修饰的地衣芽孢杆菌宿主细胞，其中一个或多个天然存在的染色体氯霉素乙酰转移酶编码基因（cat）已被灭活。 染色体cat基因的失活允许在将DNA引入修饰细胞的方法中使用氯霉素作为有效的选择剂。

公开(公告)号：US08431382B2

公开(公告)日：2013-04-30

申请号：US12302047

申请日：2007-05-29

申请人： Steen Troels Jorgensen

发明人： Steen Troels Jorgensen

IPC分类号： C12N1/20 ,  C12N15/74 ,  C12N9/10 ,  C12N15/00 ,  C12Q1/68 ,  C12P21/06 ,  C07H21/04

CPC分类号： C12N15/75 ,  C12N9/1033

摘要： The present invention relates to a modified Bacillus licheniformis host cell, wherein one or more naturally occurring chromosomal chloramphenicol acetyl transferase encoding gene(s), cat, has been inactivated. The inactivation of the chromosomal cat gene(s) allows the use of chloramphenicol as an efficient selective agent in methods for DNA introduction into the modified cell.

公开(公告)号：US20110195080A1

公开(公告)日：2011-08-11

申请号：US12523952

申请日：2009-06-24

申请人： Keith N. Haffer ,  James Larrick ,  Andrew R. Mendelsohn

发明人： Keith N. Haffer ,  James Larrick ,  Andrew R. Mendelsohn

IPC分类号： A61K39/00 ,  A61K47/26 ,  A61P3/04 ,  A61P9/00 ,  A61P5/02 ,  A61P37/04

CPC分类号： A61K39/0008 ,  A61K39/0005 ,  A61K39/39 ,  A61K2039/55555 ,  A61K2039/55566 ,  C07K14/655 ,  C07K2319/00 ,  C07K2319/31 ,  C12N9/1033

摘要： Compositions and methods are provided for treatment growth hormone and/or insulin-like growth factor 1 deficiency in a patient in need of such treatment. Compositions and methods include novel vaccines that provide immunogenicity for somatostatin and result in the increased release of endogenously produced growth hormone and/or insulin-like growth factor 1.

摘要翻译： 提供了在需要这种治疗的患者中治疗生长激素和/或胰岛素样生长因子1缺陷的组合物和方法。 组合物和方法包括为生长抑素提供免疫原性并导致内源性产生的生长激素和/或胰岛素样生长因子1的增加释放的新型疫苗。

公开(公告)号：US20090280569A1

公开(公告)日：2009-11-12

申请号：US12302047

申请日：2007-05-29

申请人： Steen Troels Jorgensen

发明人： Steen Troels Jorgensen

IPC分类号： C12N15/75 ,  C12N1/21

CPC分类号： C12N15/75 ,  C12N9/1033

摘要： The present invention relates to a modified Bacillus licheniformis host cell, wherein one or more naturally occurring chromosomal chloramphenicol acetyl transferase encoding gene(s), cat, has been inactivated. The inactivation of the chromosomal cat gene(s) allows the use of chloramphenicol as an efficient selective agent in methods for DNA introduction into the modified cell.

摘要翻译： 本发明涉及一种修饰的地衣芽孢杆菌宿主细胞，其中一个或多个天然存在的染色体氯霉素乙酰转移酶编码基因（cat）已被灭活。 染色体cat基因的失活允许在将DNA引入修饰细胞的方法中使用氯霉素作为有效的选择剂。

公开(公告)号：US06890554B2

公开(公告)日：2005-05-10

申请号：US08450555

申请日：1995-05-25

申请人： Joel A. Jessee ,  William G. Hearl

发明人： Joel A. Jessee ,  William G. Hearl

IPC分类号： G01N33/53 ,  A61K31/70 ,  A61K38/00 ,  A61K39/00 ,  A61K39/12 ,  A61K48/00 ,  A61P37/04 ,  C07H21/04 ,  C07K16/00 ,  C07K16/40 ,  C07K16/44 ,  C12N9/10 ,  C12N15/09 ,  C12N15/88 ,  C12P21/08 ,  A61K9/127

CPC分类号： C12N15/88 ,  A61K39/00 ,  A61K2039/53 ,  C07K16/00 ,  C07K16/40 ,  C12N9/1033

摘要： A method for immunization using genetic material is disclosed. Compositions for genetic immunization comprising cationic lipids and polynucleotides are also disclosed. Methods for using genetic immunization to produce polyclonal and monoclonal antibodies are also disclosed. A method for epitope mapping is also disclosed.

摘要翻译： 公开了使用遗传材料进行免疫的方法。 还公开了包含阳离子脂质和多核苷酸的遗传免疫的组合物。 还公开了使用遗传免疫来产生多克隆和单克隆抗体的方法。 还公开了表位作图的方法。

公开(公告)号：US20040266711A1

公开(公告)日：2004-12-30

申请号：US10795078

申请日：2004-03-03

申请人： Pharming B.V.

发明人： Jeffrey M. Rosen

IPC分类号： A61K048/00 ,  A01K067/00

CPC分类号： A01K67/0275 ,  A01K67/02 ,  A01K2217/05 ,  A01K2217/20 ,  A01K2227/10 ,  A01K2227/105 ,  A01K2267/01 ,  A23C9/20 ,  A23C2230/05 ,  C07K14/4732 ,  C07K2319/00 ,  C07K2319/02 ,  C07K2319/61 ,  C12N9/1033 ,  C12N15/63 ,  C12N15/85 ,  C12N15/8509 ,  C12N15/89 ,  C12N2830/008 ,  C12N2830/30 ,  C12N2830/85

摘要： Described is a method of targeting specific genes to the mammary gland which results in the efficient synthesis and secretion of biologically important molecules. Further, there is described as a composition of matter, a transgenic mammal having the ability to reproduce itself and being suitable for the secretion of biologically active agents into its milk. Additionally there is disclosed as a composition of matter, recombinant DNA gene complexes designed to integrate into a mammalian genome and to synthesize and secrete biological active agents into the milk. Furthermore, methods of producing and using altered milk are disclosed.

摘要翻译： 描述了一种靶向乳腺特异性基因的方法，其导致生物重要分子的有效合成和分泌。 此外，描述为物质组合物，转基因哺乳动物具有自身繁殖并适于将生物活性剂分泌到其乳中的能力。 另外公开了一种物质组合物，设计用于整合到哺乳动物基因组中并将生物活性剂合成并分泌到乳中的重组DNA基因复合物。 此外，公开了生产和使用改性牛奶的方法。

公开(公告)号：US20020077305A1

公开(公告)日：2002-06-20

申请号：US08450555

申请日：1995-05-25

发明人： JOEL A. JESSEE ,  WILLIAM G. HEARL

IPC分类号： A61K048/00 ,  C07H021/04 ,  C12N015/88 ,  A61K009/127

CPC分类号： C12N15/88 ,  A61K39/00 ,  A61K2039/53 ,  C07K16/00 ,  C07K16/40 ,  C12N9/1033

摘要： A method for immunization using genetic material is disclosed. Compositions for genetic immunization comprising cationic lipids and polynucleotides are also disclosed. Methods for using genetic immunization to produce polyclonal and monoclonal antibodies are also disclosed. A method for epitope mapping is also disclosed.

摘要翻译： 公开了使用遗传材料进行免疫的方法。 还公开了包含阳离子脂质和多核苷酸的遗传免疫的组合物。 还公开了使用遗传免疫来产生多克隆和单克隆抗体的方法。 还公开了表位作图的方法。

公开(公告)号：US06303340B1

公开(公告)日：2001-10-16

申请号：US09349644

申请日：1999-07-08

申请人： N. Stephen Pollitt ,  Douglas I. Buckley ,  Peter A. Stathis ,  Taymar E. Hartman ,  Ziyang Zhong

发明人： N. Stephen Pollitt ,  Douglas I. Buckley ,  Peter A. Stathis ,  Taymar E. Hartman ,  Ziyang Zhong

IPC分类号： C12P2104

CPC分类号： C07K1/36 ,  C07K1/122 ,  C07K14/58 ,  C07K2319/00 ,  C07K2319/35 ,  C07K2319/75 ,  C12N9/1033 ,  C12N15/62 ,  C12P21/06 ,  C12Y203/01028

摘要： A method is described for producing a peptide having a pI above 8 or below 5 wherein the peptide is expressed as a fusion protein in which it is linked to a fusion partner through an acid cleavage site. The peptide is released from the fusion partner by acid cleavage in the absence of chaotrope. The fusion partner and its acid cleavage products, if any, have a net charge sufficiently different from that of the desired peptide to allow isolation of the peptide by ion-exchange chromatography.

摘要翻译： 描述了一种制备pI高于8或低于5的肽的方法，其中肽被表达为通过酸切割位点与融合配偶体连接的融合蛋白。 在不存在离液剂的情况下，通过酸裂解将肽从融合配偶体释放出来。 融合配偶体及其酸裂解产物（如果有的话）具有与所需肽充分不同的净电荷，以允许通过离子交换层析分离肽。

公开(公告)号：US5879919A

公开(公告)日：1999-03-09

申请号：US56966

申请日：1998-04-08

申请人： Brian W. Grinnell

发明人： Brian W. Grinnell

IPC分类号： C12N1/38 ,  C12N9/10 ,  C12N9/64 ,  C12N9/72 ,  C12N15/85 ,  C12N15/64

CPC分类号： C12N9/6464 ,  C12N1/38 ,  C12N15/85 ,  C12N9/1033 ,  C12N9/6459 ,  C12Y304/21069 ,  C12N2799/022 ,  C12N2830/00 ,  C12N2830/002 ,  C12N2830/30 ,  C12N2830/42 ,  C12N2830/55 ,  C12N2830/60 ,  C12N2830/85 ,  C12N2840/105 ,  C12N2840/20

摘要： The present invention is a method of using the BK enhancer in tandem with a eukaryotic promoter to promote transcription of DNA that encodes a useful substance. The method of the present invention requires the presence of the E1A gene product for maximum expression of the useful substance. The present invention also comprises a number of useful expression vectors that comprise the BK enhancer in tandem with the adenovirus 2 late promoter positioned to drive expression of a variety of proteins, such as protein C, chloramphenicol acetyltransferase, and tissue plasminogen activator. The present invention further comprises a method for increasing the activity of the BK enhancer involving placement of the BK enhancer immediately upstream of the eukaryotic promoter used in tandem with the BK enhancer to drive expression of a useful substance. Furthermore, the present invention also comprises a method for coamplification of genes in primate cells. Additionally, the invention further comprises the recombinant human protein C molecule produced in 293 cells which comprises novel glycosylation patterns.

公开(公告)号：US5703055A

公开(公告)日：1997-12-30

申请号：US187630

申请日：1994-01-26

申请人： Philip L. Felgner ,  Jon Asher Wolff ,  Gary H. Rhodes ,  Robert Wallace Malone ,  Dennis A. Carson

发明人： Philip L. Felgner ,  Jon Asher Wolff ,  Gary H. Rhodes ,  Robert Wallace Malone ,  Dennis A. Carson

IPC分类号： A61K9/127 ,  A61K48/00 ,  C07K14/16 ,  C07K14/61 ,  C12N9/02 ,  C12N9/10 ,  C12N9/12 ,  C12N9/78 ,  C12N15/87 ,  A61K39/00 ,  A61K39/395 ,  C12P21/06

CPC分类号： C07K14/005 ,  A61K48/00 ,  A61K9/1272 ,  C07K14/61 ,  C12N15/87 ,  C12N9/0069 ,  C12N9/1033 ,  C12N9/1247 ,  C12N9/78 ,  C12Y113/12007 ,  C12N2740/16122

摘要： A method is disclosed for delivering an isolated polynucleotide to the interior of a cell in a vertebrate, comprising the interstitial introduction of an isolated polynucleotide into a tissue of the vertebrate where the polynucleotide is taken up by the cells of the tissue and exerts a therapeutic effect on the vertebrate. The method can be used to deliver a therapeutic polypeptide to the cells of the vertebrate, to provide an immune response upon in vivo translation of the polynucleotide, to deliver antisense polynucleotides, to deliver receptors to the cells of the vertebrate, or to provide transitory gene therapy. In particular, a method is disclosed for the generation of detectable antibodies by the direct administration to a tissue in a mammal a DNA sequence encoding a immunogen where the DNA is complexed to a cationic lipid.

摘要翻译： 公开了一种用于将分离的多核苷酸递送至脊椎动物细胞内部的方法，其包括将分离的多核苷酸间插入脊椎动物的组织中，其中所述多核苷酸被组织的细胞吸收并发挥治疗效果 在脊椎动物上。 该方法可用于将治疗性多肽递送至脊椎动物的细胞，以在多核苷酸的体内翻译中提供免疫应答，递送反义多核苷酸，将受体递送至脊椎动物的细胞，或提供短暂基因 治疗。 具体地，公开了通过直接向哺乳动物的组织施用编码免疫原的DNA序列产生可检测抗体的方法，其中DNA与阳离子脂质复合。