公开(公告)号：US06849776B1

公开(公告)日：2005-02-01

申请号：US09617543

申请日：2000-07-14

申请人： Viktor Kuvshinov ,  Kimmo Koivu ,  Anne Kanerva ,  Eija Pehu

发明人： Viktor Kuvshinov ,  Kimmo Koivu ,  Anne Kanerva ,  Eija Pehu

IPC分类号： A01H1/02 ,  A01H1/04 ,  C12N15/31 ,  C12N15/55 ,  C12N15/82 ,  A01H5/00 ,  A01H1/00

CPC分类号： C12N15/8251 ,  A01H1/02 ,  A01H1/04 ,  C12N15/8213 ,  C12N15/8241 ,  C12N15/8261 ,  C12N15/8263 ,  C12N15/8265 ,  C12N15/8287 ,  Y02A40/146

摘要： The invention is related to a method and a DNA construct for controlling transgene segregation in a sexually reproducing multicellular organism (SRMO), especially in such SRMOs which are susceptible of interbreeding with their cultured or wild-type relatives. The method and construct allows the farmer to reuse the transgenic crop without risk for escape of the transgene into the environment. The DNA construct is a recoverable block of function (RBF) system comprising at least one blocking construct (BC), and at least one means for recovering the blocked functions. The BC has a capacity of blocking at least one molecular or physiological function essential for the development and/or reproductive cycle of the SRMO. The BC is closely linked to the transgene of intereset (TGI). The blocked function is recoverable by an external treatment or intervention, which is optionally combined with one or more recovering constructs (RC). Different types of RBFs, including single, full, delayed, double, multiple and reversed delayed RBF are disclosed.

摘要翻译： 本发明涉及一种用于控制性生殖多细胞生物体（SRMO）中转基因分离的方法和DNA构建体，特别是在易受其培养或野生型亲本杂交的SRMO中。 该方法和结构允许农民重新利用转基因作物，而不会将转基因逃离到环境中的风险。 DNA构建体是包含至少一个阻断构建体（BC）的可恢复功能块（RBF）系统，以及用于恢复阻断功能的至少一种装置。 BC具有阻断SRMO的发育和/或生殖循环所必需的至少一种分子或生理功能的能力。 BC与intereset（TGI）的转基因密切相关。 阻断的功能可以通过外部治疗或干预来恢复，其可选地与一种或多种恢复构建体（RC）组合。 公开了不同类型的RBF，包括单个，全部，延迟，双重，多个和反向延迟RBF。

公开(公告)号：US06455761B1

公开(公告)日：2002-09-24

申请号：US09508371

申请日：2000-03-10

申请人： Viktor Kuvshinov ,  Kimmo Koivu ,  Anne Kanerva ,  Eija Pehu

发明人： Viktor Kuvshinov ,  Kimmo Koivu ,  Anne Kanerva ,  Eija Pehu

IPC分类号： C12N1582

CPC分类号： C12N15/8205

摘要： The present invention relates to plant biotechnology and specifically to a novel transformation protocol for obtaining transgenic turnip rape plants with Agrobacterium mediated transformation. In the protocol an internode section of the inflorescence carrying stem of mature turnip rape is used as explant.

摘要翻译： 本发明涉及植物生物技术，特别涉及用农杆菌介导的转化获得转基因萝卜油菜植物的新型转化方案。 在协议中，使用成熟萝卜油菜的花序携带茎的节间部分作为外植体。

公开(公告)号：US20090151028A1

公开(公告)日：2009-06-11

申请号：US12288791

申请日：2008-10-23

申请人： Viktor Kuvshinov ,  Anne Kanerva ,  Kimmo Koivu ,  Svetlana Kuvshinova ,  Eija Pehu

发明人： Viktor Kuvshinov ,  Anne Kanerva ,  Kimmo Koivu ,  Svetlana Kuvshinova ,  Eija Pehu

IPC分类号： A01H5/00

CPC分类号： C12N15/8205

摘要： The present invention relates to plant biotechnology and specifically to a method for genetically transforming Camelina sativa with Agrobacterium-mediated transformation system. It comprises Camelina sativa for producing homologous and heterologous recombinant products including oil and protein products and assessing and screening the efficacy of plant transformation. Also disclosed are transgenic Camelina sativa plants, seeds as well as cells, cell-lines and tissue of Camelina sativa.

摘要翻译： 本发明涉及植物生物技术，特别涉及用农杆菌介导的转化系统遗传转化Camelina sativa的方法。 它包括用于产生包括油和蛋白质产物的同源和异源重组产物的Camelina sativa，并评估和筛选植物转化的功效。 还公开了转基因Camelina sativa植物，种子以及Camelina sativa的细胞，细胞系和组织。

公开(公告)号：US20090151023A1

公开(公告)日：2009-06-11

申请号：US12290379

申请日：2008-10-30

申请人： Viktor Kuvshinov ,  Anne Kanerva ,  Kimmo Koivu ,  Svetlana Kuvshinova ,  Eija Pehu

发明人： Viktor Kuvshinov ,  Anne Kanerva ,  Kimmo Koivu ,  Svetlana Kuvshinova ,  Eija Pehu

IPC分类号： C12N15/82 ,  A01H5/00

CPC分类号： C12N15/8205

摘要： The present invention relates to plant biotechnology and specifically to a method for genetically transforming Camelina sativa with Agrobacterium-mediated transformation system. It comprises Camelina sativa for producing homologous and heterologous recombinant products including oil and protein products and assessing and screening the efficacy of plant transformation. Also disclosed are transgenic Camelina sativa plants, seeds as well as cells, cell-lines and tissue of Camelina sativa.

摘要翻译： 本发明涉及植物生物技术，特别涉及用农杆菌介导的转化系统遗传转化Camelina sativa的方法。 它包括用于产生包括油和蛋白质产物的同源和异源重组产物的Camelina sativa，并评估和筛选植物转化的功效。 还公开了转基因Camelina sativa植物，种子以及Camelina sativa的细胞，细胞系和组织。

公开(公告)号：US20120192318A1

公开(公告)日：2012-07-26

申请号：US13385329

申请日：2012-02-14

申请人： Viktor Kuvshinov ,  Anne Kanerva ,  Kimmo Koivu ,  Eija Pehu

发明人： Viktor Kuvshinov ,  Anne Kanerva ,  Kimmo Koivu ,  Eija Pehu

IPC分类号： A01H5/00 ,  A01H5/10 ,  C12N5/04

CPC分类号： C12N15/8205

摘要： In order to better aid consumers of prescription medicine and the like in efficient and rapid identification of the correct pill to be taken from among multiple containers, a novel group of articles of manufacture adapted for use with end-use prescription medicine containers at least includes a plurality of predetermined medicine purpose types corresponding to different types of medical or vitality purposes that underlying medicines are targeted to treat or address. Each article of manufacture is adapted to be coupled to an end-use, target prescription medicine container by an end-user or pharmacy function, and each article of manufacture at least includes an ideogram adapted to encourage a psychological association with, and identification of, the medicine expected to be placed in the target prescription medicine container to which the article of manufacture is adapted, and each article of manufacture is associated with a particular medicine purpose type.

摘要翻译： 为了更好地帮助处方药等的消费者高效快速地鉴定从多个容器中取出的正确药丸，适于与终端使用处方药容器一起使用的新一批制造制品至少包括 多种预定的药物目的类型对应于基础药物被针对性地治疗或解决的不同类型的医疗或活力目的。 每个制品都适用于通过终端用户或药房功能与最终用途的目标处方药容器相连，并且每个制品至少包括适于鼓励心理关联的识别， 期望将药物放置在适于制造制品的目标处方药容器中，并且每个制品与特定药物目的类型相关联。

公开(公告)号：US07910803B2

公开(公告)日：2011-03-22

申请号：US12288791

申请日：2008-10-23

申请人： Viktor Kuvshinov ,  Anne Kanerva ,  Kimmo Koivu ,  Svetlana Kuvshinova ,  Eija Pehu

发明人： Viktor Kuvshinov ,  Anne Kanerva ,  Kimmo Koivu ,  Svetlana Kuvshinova ,  Eija Pehu

IPC分类号： A01H1/00 ,  C12N15/82

CPC分类号： C12N15/8205

摘要： The present invention relates to plant biotechnology and specifically to a method for genetically transforming Camelina sativa with Agrobacterium-mediated transformation system. It comprises Camelina sativa for producing homologous and heterologous recombinant products including oil and protein products and assessing and screening the efficacy of plant transformation. Also disclosed are transgenic Camelina sativa plants, seeds as well as cells, cell-lines and tissue of Camelina sativa.

摘要翻译： 本发明涉及植物生物技术，特别涉及用农杆菌介导的转化系统遗传转化Camelina sativa的方法。 它包括用于产生包括油和蛋白质产物的同源和异源重组产物的Camelina sativa，并评估和筛选植物转化的功效。 还公开了转基因Camelina sativa植物，种子以及Camelina sativa的细胞，细胞系和组织。

公开(公告)号：US20050039229A1

公开(公告)日：2005-02-17

申请号：US10892513

申请日：2004-07-15

申请人： Viktor Kuvshinov ,  Kimmo Koivu ,  Anne Kanerva ,  Andrei Anissimov

发明人： Viktor Kuvshinov ,  Kimmo Koivu ,  Anne Kanerva ,  Andrei Anissimov

IPC分类号： A01H1/02 ,  A01H1/04 ,  C12N15/31 ,  C12N15/55 ,  C12N15/82 ,  A01H1/00

CPC分类号： C12N15/8265 ,  A01H1/02 ,  A01H1/04 ,  C12N15/8213 ,  C12N15/8241 ,  C12N15/8251 ,  C12N15/8261 ,  C12N15/8263 ,  C12N15/8267 ,  C12N15/8287 ,  C12N15/829 ,  Y02A40/146

摘要： An improved double Recoverable Block of Function (RBF) system for transgene containment with enhanced reliability is disclosed. The system includes two blocking constructs (BCs) at the opposite sides of a transgene of interest (TGI). The system comprises means for recovery of the function blocked by expression of the blocking genes of the BCs. The invention is also related to a kit comprising a cloning vector having the BCs and TGI or alternatively a place for TGI to be inserted in. Segregating (two-insert) and inducible (one-insert) types of double RBF are disclosed. The two BCs are either similar or different in their DNA and RNA structures. The two BCs may encode same protein but promoters driving the blocking genes are different and/or the coding sequence of the blocking genes is modified by codon preference change. A system comprising two BCs having different blocking genes is also disclosed. The system may consist of one or two Recovering constructs (RC), but also a system recovering without an RC is disclosed. Whether the system includes an RC or not, the recovery is induced by an external intervention that is controlled by human being.

摘要翻译： 公开了一种改进的双重可恢复功能块（RBF）系统，用于提高可靠性的转基因遏制。 该系统在感兴趣的转基因（TGI）的相对侧包括两个阻断构建体（BC）。 该系统包括用于恢复由BC的阻断基因的表达阻断的功能的装置。 本发明还涉及一种试剂盒，其包含具有BC和TGI的克隆载体或替代地待插入的TGI的位置。公开了分离（双插入）和可诱导（单插入）类型的双RBF。 两个BC在其DNA和RNA结构中是相似的或不同的。 两个BC可以编码相同的蛋白质，但是驱动阻断基因的启动子是不同的，和/或阻断基因的编码序列被密码子偏好变化修饰。 还公开了包含具有不同阻断基因的两个BC的系统。 该系统可以由一个或两个恢复构造（RC）组成，而且还公开了没有RC的系统恢复。 无论系统是否包括RC，恢复是由受人控制的外部干预引起的。

公开(公告)号：US07728192B2

公开(公告)日：2010-06-01

申请号：US11975198

申请日：2007-10-18

申请人： Kimmo Koivu ,  Viktor Kuvshinov ,  Anne Kanerva ,  Andrei Anissimov ,  Seppo Paavo Kaijalainen

发明人： Kimmo Koivu ,  Viktor Kuvshinov ,  Anne Kanerva ,  Andrei Anissimov ,  Seppo Paavo Kaijalainen

IPC分类号： C12N15/82

CPC分类号： C12N15/8222

摘要： The present invention is related to a process based on a source-sink principle, for producing products of interest from crushed or uncrushed germinating dicotyledonous seeds comprising an expression system, which is induced or can be induced during germination. The product is either a seed derived composition comprising one or more gene products. Alternatively, it is a product of interest obtained by placing the composition in contact with a substrate, containing a substance capable of being transformed by the seed derived composition as such, dried or in down-stream processed form.

摘要翻译： 本发明涉及一种基于源槽原理的方法，用于从破碎或未破碎的发芽双子叶种子产生感兴趣的产物，其包含在发芽过程中诱导或可诱导的表达系统。 产物是包含一种或多种基因产物的种子衍生组合物。 或者，通过将组合物与底物接触而获得的是令人感兴趣的产品，其含有能够由种子衍生的组合物本身转化的物质，干燥的或下游加工的形式。

公开(公告)号：US07238853B2

公开(公告)日：2007-07-03

申请号：US10644664

申请日：2003-08-20

申请人： Viktor Kuvshinov ,  Kimmo Koivu ,  Anne Kanerva ,  Andrei Anissimov

发明人： Viktor Kuvshinov ,  Kimmo Koivu ,  Anne Kanerva ,  Andrei Anissimov

IPC分类号： C12N15/82 ,  C12N5/04 ,  C12N5/10 ,  A01H5/00 ,  C12N15/31 ,  C12N15/55 ,  A01H1/02

CPC分类号： A01H1/02 ,  A01H1/04 ,  C12N15/8213 ,  C12N15/8241 ,  C12N15/8251 ,  C12N15/8261 ,  C12N15/8263 ,  C12N15/8265 ,  C12N15/8287 ,  Y02A40/146

摘要： The present invention provides a molecular mechanism for gene containment in sexually reproducing transgenic plants. The mechanism is achieved with a molecular construct comprising a blocking construct (BC) that is inserted fully or partially into an intron of a transgene of interest (TGI). The TGI encodes desired gene products, such as heterologous or homologous proteins, peptides or other useful products. The expression of the BC leads to block of at least one molecular or physiological function that is essential for development or reproduction of the transgenic plant. Thereby the BC expression leads to death or incapacity of sexual reproduction of the plant. Moreover, the mechanism comprises an externally applicable recovering tool to recover the functions blocked by the BC. The recovering tool may be a recovering construct (RC).

摘要翻译： 本发明提供了在性繁殖转基因植物中的基因遏制的分子机制。 该机制通过包含完全或部分插入感兴趣的转基因（TGI）的内含子的阻断构建体（BC）的分子构建体来实现。 TGI编码所需的基因产物，例如异源或同源蛋白质，肽或其它有用的产物。 BC的表达导致对转基因植物的发育或繁殖至关重要的至少一种分子或生理功能的阻断。 因此，BC表达导致植物的有性繁殖的死亡或丧失工作能力。 此外，该机制包括一个外部应用的恢复工具，以恢复由BC封锁的功能。 恢复工具可以是恢复构造（RC）。

公开(公告)号：US07790950B2

公开(公告)日：2010-09-07

申请号：US11632397

申请日：2005-07-05

申请人： Viktor Kuvshinov ,  Kimmo Koivu ,  Anne Kanerva ,  Andrei Anissimov

发明人： Viktor Kuvshinov ,  Kimmo Koivu ,  Anne Kanerva ,  Andrei Anissimov

IPC分类号： C12N15/82 ,  C12N15/31 ,  C12N15/52 ,  A01N45/02

CPC分类号： C12N15/8265 ,  A01H1/02 ,  A01H1/04 ,  C12N15/8213 ,  C12N15/8241 ,  C12N15/8251 ,  C12N15/8261 ,  C12N15/8263 ,  C12N15/8267 ,  C12N15/8287 ,  C12N15/829 ,  Y02A40/146

摘要： The present invention is related to a recoverable block of function (RBF) system for controlling transgene escape and transgene containment in plants. The RBF-system comprises at least one blocking construct (BC), wherein the BC comprises as the blocking gene a nucleotide sequence or crtB gene encoding phytoene synthase, which is used alone or in combination with other BCs. The crtB gene expresses under the control of a germination specific promoter or a promoter constitutively repressed by a Recovering construct (RC) and blocks the germination of a transgenic seed. The blocked germination is recoverable by a user controlled intervention including addition of an effective amount of gibberellin and sugar and may involve so called recovering constructs (RCs), wherein the recovering gene may encode ent-copalyl diphosphate synthase or ent-kaurene synthase.

摘要翻译： 本发明涉及用于控制植物中转基因逃逸和转基因遏制的可恢复功能块（RBF）系统。 RBF系统包含至少一个阻断构建体（BC），其中BC包含编码芸苔属合酶的核苷酸序列或crtB基因作为阻断基因，其单独使用或与其它BC组合使用。 crtB基因在由发芽特异性启动子或由恢复构建体（RC）组成型抑制的启动子的控制下表达，并阻断转基因种子的发芽。 封闭的萌发可以通过用户控制的干预来恢复，包括加入有效量的赤霉素和糖，并且可以涉及所谓的回收构建体（RC），其中回收基因可以编码内酰基二磷酸合成酶或ent-kaurene合酶。