公开(公告)号：US20090325259A1

公开(公告)日：2009-12-31

申请号：US12354693

申请日：2009-01-15

申请人： Viola Vogel ,  Wendy Thomas ,  Manu Forero ,  Evgeni Sokurenko

发明人： Viola Vogel ,  Wendy Thomas ,  Manu Forero ,  Evgeni Sokurenko

IPC分类号： C12N11/00 ,  C07K14/00 ,  C07K16/00 ,  C07K14/42

CPC分类号： C07K14/245 ,  A61K39/00 ,  C07K16/1232

摘要： Methods, compositions and devices are provided based on changing the binding strength of an adhesion molecule to a ligand by changing the force exerted on the bound complex between adhesion molecule and ligand, for example by changing the shear stress acting on the complex. The adhesion molecules and their ligands of this invention bind more tightly when a force-activated bond stress, such as shear force, applied to the adhesion molecules is increased, and bond less tightly when the stress is decreased. The adhesion molecules can be isolated from their sources in nature or can remain attached to their natural sources. They can be engineered, e.g., by altering their amino acid sequences or by binding to antibodies or other particles, to alter their binding properties. They can be attached to a wide range of substrates including particles and device surfaces to form adhesive systems which are capable of sticking to other particles and/or device surfaces to which ligands for the adhesion molecules have been attached. The adhesion molecules and ligands described herein can be used to control binding and release of components of an adhesive system by increasing or decreasing the force-activated bond stresses applied to the adhesion molecules.

摘要翻译： 基于通过改变施加在粘附分子和配体之间的结合复合物上的力，例如通过改变作用在复合物上的剪切应力来改变粘附分子与配体的结合强度的方法，组合物和装置。 当施加到粘附分子上的力 - 活化的粘合应力（例如剪切力）增加时，本发明的粘合分子及其配体更紧密地结合，并且当应力降低时，粘合力较弱。 粘附分子可以在自然界中从其来源中分离出来，或者可以保持附着于其天然来源。 它们可以例如通过改变它们的氨基酸序列或通过结合抗体或其它颗粒来改造它们的结合性质。 它们可以连接到包括颗粒和装置表面在内的各种基材上以形成粘合剂体系，该粘合剂体系能够粘附到附着有粘附分子的配体的其它颗粒和/或装置表面。 本文所述的粘附分子和配体可用于通过增加或减少施加于粘附分子的力 - 活化的粘合应力来控制粘合剂体系的组分的结合和释放。