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公开(公告)号：US11414372B2
公开(公告)日：2022-08-16
申请号：US16252915
申请日：2019-01-21
发明人： John Ralph , Steven D Karlen , Fachuang Lu , Dharshana Padmakshan
IPC分类号： C07C69/00 , C07C69/73 , C07C69/007 , C07C69/16 , C07C69/732 , C07C69/734 , C07C69/84 , C07C69/86 , C07C69/92 , G01N33/50
摘要： Methods of detecting and, optionally, determining a level of incorporation of monolignol ester conjugates into lignin. The methods include derivatizing lignin to acylate at least a portion of free phenolic and aliphatic hydroxyls and to halogenate at least a portion of benzylic alcohols present in the lignin to yield derivatized lignin, treating the derivatized lignin with a reducing agent to cleave at least a portion of the derivatized lignin to yield lignin cleavage products, acetylating at least a portion of free hydroxyl groups in the lignin cleavage products with a labeled acetylation agent to yield labeled lignin fragments, and detecting the labeled lignin fragments.
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2.发明申请METHODS FOR DETECTING AND DETERMINING LEVELS OF MONOLIGNOL ESTER CONJUGATES INCORPORATED INTO LIGNIN AND COMPOUNDS RELATING THERETO 审中-公开用于检测和测定与其相关的葡萄糖和化合物的单烯醇酯共聚物的水平的方法公开(公告)号：US20160187345A1
公开(公告)日：2016-06-30
申请号：US14985720
申请日：2015-12-31
发明人： John Ralph , Steven D Karlen , Fachuang Lu , Dharshana Padmakshan
CPC分类号： C07C69/73 , C07B2200/05 , C07C69/007 , C07C69/16 , C07C69/732 , C07C69/734 , C07C69/84 , C07C69/86 , C07C69/92 , G01N33/50 , G01N2400/26 , G01N2458/15 , G01N2560/00
摘要： Described is a method of detecting and/or determining levels of incorporation of monolignol ferulate conjugate esters into lignin by derivatizing the lignin, reductively cleaving the lignin, labeling the cleavage products, and then detecting cleavage products derived from monolignol ester conjugates via differential attachment of the label.
摘要翻译： 描述了通过衍生木质素，还原性切割木质素，标记裂解产物，然后通过差异附着来检测衍生自单螺环酯酯缀合物的裂解产物，来检测和/或确定单胺阿魏酸酯偶联物酯掺入木质素中的水平的方法 标签。
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公开(公告)号：US10227287B2
公开(公告)日：2019-03-12
申请号：US14985720
申请日：2015-12-31
发明人： John Ralph , Steven D Karlen , Fachuang Lu , Dharshana Padmakshan
IPC分类号： C07C69/73 , C07C69/007 , C07C69/16 , C07C69/732 , C07C69/734 , C07C69/84 , C07C69/86 , C07C69/92 , G01N33/50
摘要： Described is a method of detecting and/or determining levels of incorporation of monolignol ferulate conjugate esters into lignin by derivatizing the lignin, reductively cleaving the lignin, labeling the cleavage products, and then detecting cleavage products derived from monolignol ester conjugates via differential attachment of the label.
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