公开(公告)号：US11740236B2

公开(公告)日：2023-08-29

申请号：US16158506

申请日：2018-10-12

申请人： Massachusetts Institute of Technology

发明人： Hadley Sikes Johnson ,  Eric Alexander Miller ,  Ki-Joo Sung

IPC分类号： G01N33/566 ,  C07K14/33 ,  G01N33/569 ,  C07K14/195 ,  C07K16/06 ,  C07K16/28

CPC分类号： G01N33/566 ,  C07K14/195 ,  C07K14/33 ,  C07K16/065 ,  C07K16/28 ,  G01N33/56911 ,  C07K2319/20 ,  C07K2319/24 ,  C07K2319/60 ,  G01N2400/26

摘要： The present disclosure relates to proteins comprising a target-binding domain for detection of a target of interest, methods, compositions and kits thereof.

公开(公告)号：US20170115267A1

公开(公告)日：2017-04-27

申请号：US15332588

申请日：2016-10-24

申请人： Buckman Laboratories International, Inc. ,  Universite du Quebec a Trois-Rivieres

发明人： Marc Beauregard ,  Fatma Meddeb-Mouelhi ,  Yannick Hebert-Ouellet ,  Bernard Janse ,  Kevin J. MacDonald

IPC分类号： G01N33/34 ,  C07K14/00

CPC分类号： G01N33/343 ,  C07K14/001 ,  D21H23/76 ,  G01N33/5308 ,  G01N2400/26

摘要： A polymer detection probe is provided that includes a binding module that specifically binds to at least one polymer and a reporter module that is spectroscopically detectable. The binding module can be a carbohydrate-binding module (CBM). The reporter module can be a fluorescent protein. A complex is provided that includes a probe specifically bound to a pulp or paper product including at least one surface available lignocellulosic polymer. A pulp or paper product is provided that includes at least one surface available lignocellulosic polymer and at least one probe bound thereto. Methods are provided that employ a lignocellulosic probe. A method of detecting a lignocellulosic polymer or other type of polymer is provided. A method of determining the effectiveness of an industrial treatment on pulp or a paper product is also provided. A method of determining a physical property of pulp or a paper product is further provided.

公开(公告)号：US20160187345A1

公开(公告)日：2016-06-30

申请号：US14985720

申请日：2015-12-31

申请人： WISCONSIN ALUMNI RESEARCH FOUNDATION

发明人： John Ralph ,  Steven D Karlen ,  Fachuang Lu ,  Dharshana Padmakshan

IPC分类号： G01N33/58 ,  C07C69/73 ,  C07C69/78

CPC分类号： C07C69/73 ,  C07B2200/05 ,  C07C69/007 ,  C07C69/16 ,  C07C69/732 ,  C07C69/734 ,  C07C69/84 ,  C07C69/86 ,  C07C69/92 ,  G01N33/50 ,  G01N2400/26 ,  G01N2458/15 ,  G01N2560/00

摘要： Described is a method of detecting and/or determining levels of incorporation of monolignol ferulate conjugate esters into lignin by derivatizing the lignin, reductively cleaving the lignin, labeling the cleavage products, and then detecting cleavage products derived from monolignol ester conjugates via differential attachment of the label.

摘要翻译： 描述了通过衍生木质素，还原性切割木质素，标记裂解产物，然后通过差异附着来检测衍生自单螺环酯酯缀合物的裂解产物，来检测和/或确定单胺阿魏酸酯偶联物酯掺入木质素中的水平的方法 标签。

公开(公告)号：US20030132538A1

公开(公告)日：2003-07-17

申请号：US10355073

申请日：2003-01-31

申请人： LUMINEX CORPORATION

发明人： Don J. Chandler

IPC分类号： B01J013/02

CPC分类号： G01N33/587 ,  B01J13/046 ,  B01J13/18 ,  C12Q2563/107 ,  C12Q2563/149 ,  G01N2015/1018 ,  G01N2333/78 ,  G01N2400/26 ,  G01N2400/28 ,  Y10T428/2984 ,  Y10T428/2985 ,  Y10T428/2991

摘要： The present invention provides novel encapsulation compositions and methods. In particular, the invention relates to fluorescent capsule compositions, which consists of a layer of a polymer shell enclosing one or more fluorescent materials such as fluorescent microspheres and which are capable of emitting at least two distinct fluorescent signals. Also provided are methods for their preparation. The compositions and methods of this invention are useful in a variety of applications, including preparation of multiplexed arrays for industrial, chemical, immunological, and genetic manipulation and analysis especially as related but not limited to flow cytometry.

公开(公告)号：US6124117A

公开(公告)日：2000-09-26

申请号：US788621

申请日：1997-01-23

申请人： Douglas G. Kilburn ,  Robert C. Miller ,  Richard A. J. Warren ,  Neil R. Gilkes

发明人： Douglas G. Kilburn ,  Robert C. Miller ,  Richard A. J. Warren ,  Neil R. Gilkes

IPC分类号： G01N33/573 ,  A61K39/39 ,  C07K14/55 ,  C07K17/12 ,  C07K19/00 ,  C08B15/00 ,  C08B37/08 ,  C08H8/00 ,  C12N9/16 ,  C12N9/38 ,  C12N9/42 ,  C12N11/12 ,  C12N15/09 ,  C12P21/02 ,  C12Q1/56 ,  C12R1/19 ,  D06P3/60 ,  D21H17/00 ,  G01N33/531 ,  C12N15/56

CPC分类号： C08H8/00 ,  A61K39/39 ,  C07K14/55 ,  C08B15/00 ,  C08B37/003 ,  C12N11/12 ,  C12N9/16 ,  C12N9/2437 ,  C12N9/2468 ,  C12Q1/56 ,  C12Y302/01004 ,  C12Y302/01074 ,  C12Y302/01091 ,  D21H17/005 ,  G01N33/531 ,  C07K2319/00 ,  C07K2319/02 ,  C07K2319/034 ,  C07K2319/33 ,  C07K2319/50 ,  C07K2319/75 ,  G01N2333/924 ,  G01N2400/26 ,  Y10S435/803 ,  Y10S530/808 ,  Y10S530/814

摘要： Novel polypeptide compositions and methods for their use are provided comprising fusion proteins in which the polysaccharide binding domain or functional portion thereof of a polysaccharidase is fused to a heterologous protein or is conjugated to a chemical moiety. The compositions can be synthesized or prepared by recombinant DNA technology. The compositions find use as removable labels.

摘要翻译： 提供了新的多肽组合物及其使用方法，其包含融合蛋白，其中多糖酶的多糖结合结构域或其功能部分与异源蛋白融合或与化学部分缀合。 可以通过重组DNA技术合成或制备组合物。 该组合物用作可移除标签。

公开(公告)号：US5962289A

公开(公告)日：1999-10-05

申请号：US788622

申请日：1997-01-24

申请人： Douglas G. Kilburn ,  Robert C. Miller ,  Richard A.J. Warren ,  Neil R. Gilkes

发明人： Douglas G. Kilburn ,  Robert C. Miller ,  Richard A.J. Warren ,  Neil R. Gilkes

IPC分类号： G01N33/573 ,  A61K39/39 ,  C07K14/55 ,  C07K17/12 ,  C07K19/00 ,  C08B15/00 ,  C08B37/08 ,  C08H8/00 ,  C12N9/16 ,  C12N9/38 ,  C12N9/42 ,  C12N11/12 ,  C12N15/09 ,  C12P21/02 ,  C12Q1/56 ,  C12R1/19 ,  D06P3/60 ,  D21H17/00 ,  G01N33/531 ,  C12N15/00 ,  C12P21/00

CPC分类号： C08H8/00 ,  A61K39/39 ,  C07K14/55 ,  C08B15/00 ,  C08B37/003 ,  C12N11/12 ,  C12N9/16 ,  C12N9/2437 ,  C12N9/2468 ,  C12Q1/56 ,  C12Y302/01004 ,  C12Y302/01074 ,  C12Y302/01091 ,  D21H17/005 ,  G01N33/531 ,  C07K2319/00 ,  C07K2319/02 ,  C07K2319/034 ,  C07K2319/33 ,  C07K2319/50 ,  C07K2319/75 ,  G01N2333/924 ,  G01N2400/26 ,  Y10S435/803 ,  Y10S530/808 ,  Y10S530/814

摘要： Fusion proteins or conjugates are provided containing an amino acid sequence having a substrate binding region of a polysaccharidase such as cellulase that binds to a .beta.-1,4-glycan matrix such as cellulose. The substrate binding region is essentially without polysaccharidase activity. In the fusion protein, the substrate binding region is fused or chemically linked to a polypeptide such as an enzyme, a hormone, an immunoglobulin or a protein dye. By contacting the fusion protein with a .beta.-1,4-glycan matrix, the substrate binding region binds to the matrix to immobilize the polypeptide on the matrix. The polypeptide or fusion protein can be removed from the matrix with a protease acting on a protease recognition sequence or with a solution having a low ionic strength or high pH. In the conjugate, the substrate binding region is joined such as by covalent bonding to a non-protein chemical moiety such as a dye, chromophore, fluorescor, radionuclide or enzyme co-factor. By contacting the conjugate with a .beta.-1,4-glycan matrix, the substrate binding region binds to the matrix to immobilize the chemical moiety on the matrix. The conjugate or chemical moiety can be removed with a protease acting on a protease recognition sequence or with a solution having a low ionic strength or high pH.

摘要翻译： 提供融合蛋白或缀合物，其含有具有多糖酶的底物结合区的氨基酸序列，例如结合β-1,4-聚糖基质如纤维素的纤维素酶。 底物结合区基本上不含多糖酶活性。 在融合蛋白中，底物结合区域与诸如酶，激素，免疫球蛋白或蛋白质染料的多肽融合或化学连接。 通过使融合蛋白与β-1,4-聚糖基质接触，底物结合区结合基质将多肽固定在基质上。 多肽或融合蛋白可以用作用于蛋白酶识别序列的蛋白质或具有低离子强度或高pH的溶液从基质中除去。 在缀合物中，底物结合区域例如通过共价键连接到非蛋白质化学部分如染料，发色团，荧光，放射性核素或酶辅因子上。 通过使该缀合物与β-1,4-聚糖基质接触，底物结合区域与基质结合以将化学部分固定在基质上。 缀合物或化学部分可以用作用于蛋白酶识别序列的蛋白酶或具有低离子强度或高pH的溶液来除去。

公开(公告)号：US5340731A

公开(公告)日：1994-08-23

申请号：US865095

申请日：1992-04-08

申请人： Douglas G. Kilburn ,  Robert C. Miller ,  Neil Gilkes ,  R. Antony J. Warren

发明人： Douglas G. Kilburn ,  Robert C. Miller ,  Neil Gilkes ,  R. Antony J. Warren

IPC分类号： G01N33/573 ,  A61K39/39 ,  C07K14/55 ,  C07K17/12 ,  C07K19/00 ,  C08B15/00 ,  C08B37/08 ,  C08H8/00 ,  C12N9/16 ,  C12N9/38 ,  C12N9/42 ,  C12N11/12 ,  C12N15/09 ,  C12P21/02 ,  C12Q1/56 ,  C12R1/19 ,  D06P3/60 ,  D21H17/00 ,  G01N33/531 ,  C12N15/00 ,  C12P21/00

CPC分类号： C08H8/00 ,  A61K39/39 ,  C07K14/55 ,  C08B15/00 ,  C08B37/003 ,  C12N11/12 ,  C12N9/16 ,  C12N9/2437 ,  C12N9/2468 ,  C12Q1/56 ,  C12Y302/01004 ,  C12Y302/01074 ,  C12Y302/01091 ,  D21H17/005 ,  G01N33/531 ,  C07K2319/00 ,  C07K2319/02 ,  C07K2319/034 ,  C07K2319/33 ,  C07K2319/50 ,  C07K2319/75 ,  G01N2333/924 ,  G01N2400/26 ,  Y10S435/803 ,  Y10S530/808 ,  Y10S530/814

摘要： A fusion protein that can function as a removable label is prepared containing a polypetide such as an enzyme and an amino acid sequence having a substrate binding region of a polysaccharidase such as cellulase that has essentially no polysaccharidase activity. By contacting the fusion protein with a .beta.-1,4 glycan matrix such as cellulose, the substrate binding region binds to the matrix to immobilize the polypeptide. The polypetide or fusion protein can be removed from the matrix with a protease capable of cleaving a specific protease cleavage site, or with a solution having a low ionic strength or a high pH. The fusion protein can be prepared by recombinant DNA technology.

摘要翻译： 制备可用作可去除标记的融合蛋白，其含有多肽如酶和具有基本上不含多糖酶活性的纤维素酶的多糖酶的底物结合区的氨基酸序列。 通过使融合蛋白与β-1,4-聚糖基质例如纤维素接触，底物结合区结合基质以固定多肽。 可以用能够切割特异性蛋白酶切割位点的蛋白质或用离子强度低或高pH值的溶液从基质中除去多肽或融合蛋白。 融合蛋白可以通过重组DNA技术制备。

公开(公告)号：US20150293082A1

公开(公告)日：2015-10-15

申请号：US14750970

申请日：2015-06-25

申请人： Asahi Kasei Fibers Corporation

发明人： Yoshiyuki SHIOMI ,  Toshihiko Matsui ,  Masanori Doi

IPC分类号： G01N33/548

CPC分类号： G01N33/548 ,  C08B1/006 ,  C08B1/08 ,  C08B5/14 ,  C08B11/02 ,  C08B11/04 ,  C08B11/08 ,  C08B11/10 ,  C08B11/12 ,  C08B11/145 ,  C08B11/155 ,  C08B11/20 ,  C08B15/005 ,  C08B15/04 ,  C08B15/10 ,  C08B16/00 ,  C08J3/16 ,  C08J2301/28 ,  C08L1/08 ,  C08L1/26 ,  C08L1/28 ,  C08L1/282 ,  C08L1/284 ,  C08L1/286 ,  C08L1/288 ,  G01N2400/26 ,  Y10T428/2982

摘要： An object of the present invention is to provide a hydrophilic cellulose derivative fine particle having a small particle size, a dispersion liquid thereof and a dispersion body thereof; and provide a diagnostic reagent composed of the hydrophilic particle, which is excellent in storage stability and does not require excess components, such as an emulsifier or surfactant. The cellulose derivative fine particle of the present invention is a cellulose derivative fine particle comprising a cellulose derivative with a part of hydroxyl groups of cellulose being substituted with a substituent, wherein the average particle diameter is from 9 to 1,000 nm; and the diagnostic reagent of the present invention is a diagnostic reagent obtained by loading a substance differentially interacting with a test object substance on the above-described cellulose derivative fine particle.

摘要翻译： 本发明的目的是提供一种具有小粒径的亲水性纤维素衍生物微粒，其分散液及其分散体; 并且提供由亲水性颗粒组成的诊断试剂，其具有优异的储存稳定性，并且不需要过量的组分如乳化剂或表面活性剂。 本发明的纤维素衍生物微粒是由纤维素衍生物构成的纤维素衍生物微粒，其纤维素的一部分羟基被取代基取代，平均粒径为9〜1000nm， 本发明的诊断试剂是通过将与试验对象物质差异相互作用的物质负载在上述纤维素衍生物微粒上而得到的诊断试剂。

公开(公告)号：US06905766B2

公开(公告)日：2005-06-14

申请号：US10355073

申请日：2003-01-31

申请人： Don J. Chandler

发明人： Don J. Chandler

IPC分类号： B01J13/04 ,  B01J13/18 ,  G01N15/10 ,  G01N33/58 ,  B32B15/02 ,  B01J13/02

CPC分类号： G01N33/587 ,  B01J13/046 ,  B01J13/18 ,  C12Q2563/107 ,  C12Q2563/149 ,  G01N2015/1018 ,  G01N2333/78 ,  G01N2400/26 ,  G01N2400/28 ,  Y10T428/2984 ,  Y10T428/2985 ,  Y10T428/2991

摘要： The present invention provides novel encapsulation compositions and methods. In particular, the invention relates to fluorescent capsule compositions, which consists of a layer of a polymer shell enclosing one or more fluorescent materials such as fluorescent microspheres and which are capable of emitting at least two distinct fluorescent signals. Also provided are methods for their preparation. The compositions and methods of this invention are useful in a variety of applications, including preparation of multiplexed arrays for industrial, chemical, immunological, and genetic manipulation and analysis especially as related but not limited to flow cytometry.

摘要翻译： 本发明提供了新颖的包封组合物和方法。 特别地，本发明涉及荧光胶囊组合物，其由包围一种或多种荧光材料如荧光微球的聚合物壳层组成，并且能够发射至少两种不同的荧光信号。 还提供了它们的制备方法。 本发明的组合物和方法可用于各种应用，包括制备用于工业，化学，免疫和遗传操作和分析的复合阵列，特别是相关但不限于流式细胞术。

公开(公告)号：US06528165B2

公开(公告)日：2003-03-04

申请号：US10103807

申请日：2002-03-25

申请人： Don J. Chandler

发明人： Don J. Chandler

IPC分类号： B32B1502

CPC分类号： G01N33/587 ,  B01J13/046 ,  B01J13/18 ,  C12Q2563/107 ,  C12Q2563/149 ,  G01N2015/1018 ,  G01N2333/78 ,  G01N2400/26 ,  G01N2400/28 ,  Y10T428/2984 ,  Y10T428/2985 ,  Y10T428/2991

摘要： The present invention provides novel encapsulation compositions and methods. In particular, the invention relates to fluorescent capsule compositions, which consists of a layer of a polymer shell enclosing one or more fluorescent materials such as fluorescent microspheres and which are capable of emitting at least two distinct fluorescent signals. Also provided are methods for their preparation. The compositions and methods of this invention are useful in a variety of applications, including preparation of multiplexed arrays for industrial, chemical, immunological, and genetic manipulation and analysis especially as related but not limited to flow cytometry.