公开(公告)号：US20020038001A1

公开(公告)日：2002-03-28

申请号：US09865171

申请日：2001-05-24

Applicant: Washington State University Research Foundation

Inventor： Rodney Bruce Croteau ,  Jorg Bohlmann ,  John E. Crock ,  Christopher L. Steele

IPC: C12P021/00

CPC classification number: C12N15/8243 ,  C12N9/88 ,  C12N15/8279

Abstract: cDNAs encoding E-null-bisabolelene synthase, null-selinene synthase and null-humulene synthase from Grand Fir (Abies grandis) have been isolated and sequenced, and the corresponding amino acid sequences have been determined. Accordingly, isolated DNA sequences (SEQ ID No:12; SEQ ID No:19 and SEQ ID No:23) are provided which code for the expression of E-null-bisabolene synthase (SEQ ID No:13), null-selinene synthase (SEQ ID No:20) and null-humulene synthase (SEQ ID No:24), respectively, from Grand Fir (Abies grandis). In other aspects, replicable recombinant cloning vehicles are provided which code for E-null-bisabolene synthase, null-selinene synthase and null-humulene synthase, or for a base sequence sufficiently complementary to at least a portion of E-null-bisabolene synthase, null-selinene synthase or null-humulene synthase DNA or RNA to enable hybridization therewith. In yet other aspects, modified host cells are provided that have been transformed, transfected, infected and/or injected with a recombinant cloning vehicle and/or DNA sequence encoding E-null-bisabolene synthase, null-selinene synthase or null-humulene synthase. Thus, systems and methods are provided for the recombinant expression of the aforementioned recombinant sesquiterpene synthases that may be used to facilitate their production, isolation and purification in significant amounts. Recombinant E-null-bisabolene synthase, null-selinene synthase and null-humulene synthase may be used to obtain expression or enhanced expression of E-null-bisabolene synthase, null-selinene synthase and null-humulene synthase in plants in order to enhance the production of sesquiterpenoids, or may be otherwise employed for the regulation or expression of E-null-bisabolene synthase, null-selinene synthase and null-humulene synthase, or the production of their products.