公开(公告)号：US20020043462A1

公开(公告)日：2002-04-18

申请号：US09885439

申请日：2001-06-19

Applicant: Washington State University Research Foundation

Inventor： Cornelius F. Ivory ,  Zheng Huang ,  Fred J. Schuetze

IPC: G01N027/26 ,  G01N027/447

CPC classification number: G01N27/44795 ,  G01N27/44743 ,  G01N27/44773

Abstract: An electrophoretic device and method for focusing a charged solute is disclosed. The device includes a first chamber for receiving a fluid medium, the first chamber having an inlet for introducing a first liquid to the chamber and an outlet for exiting the first liquid from the chamber; a second chamber comprising an electrode array, the second chamber having an inlet for introducing a second liquid to the chamber and an outlet for exiting the second liquid from the chamber; and a porous material separating the first and second chambers. The device's electrode array includes a plurality of electrodes and generates an electric field gradient profile which can be dynamically controlled. In the method, a charged solute is introduced into a fluid medium followed by the application of a hydrodynamic force. Opposing the hydrodynamic force with an electric field gradient results in solute focusing in the fluid medium. The electric field gradient is generated by an electrode array by individually adjusting the electrode voltages.

Abstract translation: 公开了一种用于聚焦带电溶质的电泳装置和方法。 所述装置包括用于接收流体介质的第一室，所述第一室具有用于将第一液体引入所述室的入口和用于从所述室离开所述第一液体的出口; 包括电极阵列的第二腔室，所述第二腔室具有用于将第二液体引入所述腔室的入口和用于从所述腔室排出所述第二液体的出口; 以及分离第一和第二室的多孔材料。 器件的电极阵列包括多个电极，并产生可以动态控制的电场梯度分布。 在该方法中，将带电溶质引入流体介质中，随后施加流体动力。 用电场梯度对流动力产生流体介质中的溶质聚焦。 通过单独调节电极电压由电极阵列产生电场梯度。