公开(公告)号：US20180036729A1

公开(公告)日：2018-02-08

申请号：US15670896

申请日：2017-08-07

Applicant: LI-COR, Inc.

Inventor： Michael D. Furtaw ,  Donald T. Lamb

IPC: B01L3/00 ,  G01N27/447

CPC classification number: B01L3/50273 ,  B01L2300/0681 ,  B01L2300/0829 ,  B01L2400/0421 ,  B01L2400/0478 ,  G01N27/44721 ,  G01N27/44739 ,  G01N27/44773 ,  G01N27/44782 ,  G01N27/44791

Abstract: Devices and methods are provided for the separation and dispensing of material using a microfluidic electrophoresis column, sheath liquid pump, and exit channel, all on the same monolithic chip. Material is separated in the electrophoresis column and passed into the exit chamber in response to a voltage potential between a first electrode within the electrophoresis column and a terminating electrode integrated into the chip. The terminating electrode can be in the sheath liquid pump chamber, the sheath liquid reservoir, or a separate flow channel that intersects the exit channel along with the electrophoresis column and sheath liquid pump chamber. The flow of sheath liquid into the exit chamber entrains separated analytes into an effluent that is dispensed out of the exit chamber via a discharge outlet.

公开(公告)号：US20170240882A1

公开(公告)日：2017-08-24

申请号：US15519516

申请日：2015-10-15

Applicant: Ezra S. ABRAMS ,  Danny YUN ,  Todd J. BARBERA ,  Douglas Grosvenor SABIN ,  T. Christian BOLES ,  SAGE SCIENCE, INC.

Inventor： Ezra S. ABRAMS ,  Danny YUN ,  Todd J. BARBERA ,  Douglas Grosvenor SABIN ,  T. Christian BOLES

IPC: C12N15/10 ,  G01N27/447 ,  C12Q1/68

CPC classification number: C12N15/101 ,  C12M47/06 ,  C12N15/1017 ,  C12Q1/6806 ,  G01N27/44747 ,  G01N27/44773 ,  G01N27/44782

Abstract: Methods, systems and apparatus for automated extraction, purification, and processing of nucleic acids from biological samples are presented. In some embodiments, hydrogel supports are used to immobilize particulate biological input samples and extract nucleic acids during operations. The use of hydrogel facilitates automated sample processing on robotic liquid handling systems. Devices, methods, and systems are also provided for electrophoretic sample preparation.

公开(公告)号：US09718676B2

公开(公告)日：2017-08-01

申请号：US12440546

申请日：2007-09-12

Applicant: Steven A. Soper ,  Robin L. McCarley ,  Guofang Chen ,  Hamed Shadpour

Inventor： Steven A. Soper ,  Robin L. McCarley ,  Guofang Chen ,  Hamed Shadpour

IPC: B82B3/00 ,  G01N27/26 ,  G01N33/561 ,  B81C1/00 ,  G01N27/447

CPC classification number: B81C1/00111 ,  B81C2201/034 ,  G01N27/44773 ,  Y10T428/2982

Abstract: A method is disclosed for fabricating free-standing polymeric nanopillars or nanotubes with remarkably high aspect ratios. The nanopillars and nanotubes may be used, for example, in integrated microfluidic systems for rapid, automated, high-capacity analysis or separation of complex protein mixtures or their enzyme digest products. One embodiment, preferably fabricated entirely from polymer substrates, comprises a cell lysis unit; a solid-phase extraction unit with free-standing, polymeric nanostructures; a multi-dimensional electrophoretic separation unit with high peak capacity; a solid-phase nanoreactor for the proteolytic digestion of isolated proteins; and a chromatographic unit for the separation of peptide fragments from the digestion of proteins. The nanopillars and nanotubes may also be used to increase surface area for reaction with a solid phase, for example, with immobilized enzymes or other catalysts within a microchannel, or as a solid support for capillary electrochromatography-based separations of proteins or peptides.

公开(公告)号：US20160334364A1

公开(公告)日：2016-11-17

申请号：US15103362

申请日：2014-12-04

Applicant: SHARP KABUSHIKI KAISHA

Inventor： Hideki KINOSHITA ,  Kimihiko YABE ,  Yoshiyuki ISHIDA

IPC: G01N27/447

CPC classification number: G01N27/44747 ,  G01N27/44773 ,  G01N27/44778 ,  G01N27/44795

Abstract: There are provided (i) a gel for use in polyacrylamide gel electrophoresis which gel produces a concentration effect for a longer time period and prevents heat generation during migration and (ii) an electrophoresis apparatus provided with the gel. A gel according to the present invention for use in polyacrylamide gel electrophoresis includes a concentration gel and a separation gel having a pH adjusted to a value different from the pH value of the concentration gel, an acrylamide buffer being covalently bonded to at least one of the concentration gel and the separation gel.

Abstract translation: 提供（i）用于聚丙烯酰胺凝胶电泳中的凝胶，其凝胶在较长时间内产生浓度效应，并防止迁移过程中产生热量，以及（ii）提供凝胶的电泳装置。 根据本发明的用于聚丙烯酰胺凝胶电泳的凝胶包括浓度凝胶和pH调节至与浓度凝胶的pH值不同的值的分离凝胶，丙烯酰胺缓冲液共价键合至至少一个 浓缩凝胶和分离凝胶。

公开(公告)号：US09437411B2

公开(公告)日：2016-09-06

申请号：US14977584

申请日：2015-12-21

Applicant: COMMISSARIAT A L'ENERGIE ATOMIQUE ET AUX ENERGIES ALTERNATIVES (CEA)

Inventor： Frédéric Chartier ,  Laurent Vio ,  Anthony Nonell ,  Hélène Isnard

IPC: G01N27/447 ,  G01N27/26 ,  B01D57/02 ,  H01J49/10 ,  H01J49/00 ,  B01D59/42 ,  G01N27/62

CPC classification number: H01J49/105 ,  B01D59/42 ,  G01N27/44765 ,  G01N27/44773 ,  G01N27/622 ,  H01J49/0031

Abstract: A method for separating electrically charged species contained in a solution by an isotachophoresis method applied in an electrophoresis device, the isotachophoresis method being coupled to isotopic measurement using a mass spectrometer. The method notably comprises a step of stopping the voltage applied to the terminal of the electrophoresis capillary and transient application of a counter-pressure making it possible to utilize the length of the capillary several times and extend the separation distance artificially.

Abstract translation: 通过在电泳装置中应用的等速电泳法分离溶液中所含的带电物质的方法，使用质谱联用同位素测定法进行等速电泳法。 该方法特别包括停止施加到电泳毛细管末端的电压和瞬时施加反压的步骤，使得可以多次利用毛细管的长度并延长人工分离距离。

公开(公告)号：US09400260B2

公开(公告)日：2016-07-26

申请号：US14273508

申请日：2014-05-08

Applicant: Man-Hee Suh ,  Valentin Petrov

Inventor： Man-Hee Suh ,  Valentin Petrov

IPC: G01N27/447 ,  G01N27/453

CPC classification number: G01N27/44713 ,  G01N27/44756 ,  G01N27/44773

Abstract: An electrophoresis system is provided. The system includes a buffer chamber box having a front surface. The buffer chamber box is divided into a first chamber and a second chamber by a divider. The system also includes a gel plate. A gel chamber is formed by the gel plate and the front surface of the buffer chamber box. The first chamber has a first electrode and a first conductive path member. The second chamber has a second electrode and a second conductive path member.

Abstract translation: 提供电泳系统。 该系统包括具有前表面的缓冲室箱。 缓冲室箱被分隔件分成第一室和第二室。 该系统还包括凝胶板。 由凝胶板和缓冲室箱的前表面形成凝胶室。 第一室具有第一电极和第一导电路径构件。 第二室具有第二电极和第二导电路径构件。

公开(公告)号：US20160160211A1

公开(公告)日：2016-06-09

申请号：US14873319

申请日：2015-10-02

Applicant: UNIVERSITY OF NOTRE DAME DU LAC

Inventor： Bonnie Jaskowski Huge ,  Ryan John Flaherty ,  Oluwatosin O. Dada ,  Norman J. Dovichi

IPC: C12N15/10 ,  G01N27/447

CPC classification number: G01N27/44773 ,  G01N27/44791

Abstract: The present disclosure describes an apparatus for identifying a polynucleotide capable of binding a target. The apparatus comprises a first, second, and third high voltage power supply; a first, second, and third capillary tube; a first, second, and third buffer reservoir; a fraction collector, and at least one collection vessel.

Abstract translation: 本公开描述了用于鉴定能够结合靶标的多核苷酸的装置。 该装置包括第一，第二和第三高压电源; 第一，第二和第三毛细管; 第一，第二和第三缓冲储存器; 馏分收集器和至少一个收集容器。

公开(公告)号：US09126136B2

公开(公告)日：2015-09-08

申请号：US13928200

申请日：2013-06-26

Applicant: Zheng Ye ,  William D. Mathers ,  Ed Brightman

Inventor： Zheng Ye ,  William D. Mathers ,  Ed Brightman

IPC: B01D57/02 ,  G01N27/447 ,  G01N33/68

CPC classification number: B01D57/02 ,  G01N27/44756 ,  G01N27/44773 ,  G01N33/6803

Abstract: Cassette bodies for use with electrophoresis apparatus can be formed of a single piece of molded or machined plastic. Such cassette bodies can include a plurality of channels that pass through the cassette body, from a proximal end to a distal end. Such channels can be defined by upper and lower chambers. The upper chambers can be in fluid communication with a first buffer pool through a semi-permeable membrane, and the lower chambers can be in fluid communication with a second buffer pool. An electric current can be passed through the first and second buffer pools, and then reversed, to perform an electrophoresis operation that can separate a biomolecule of interest from free probes, and provide for convenient collection of said biomolecule of interest.

Abstract translation: 用于电泳装置的盒体可以由一片模制或机械加工的塑料制成。 这种盒体可以包括从近端到远端穿过盒主体的多个通道。 这样的通道可以由上部和下部室限定。 上室可以通过半透膜与第一缓冲池流体连通，并且下室可以与第二缓冲池流体连通。 电流可以通过第一和第二缓冲池，然后反向，以执行电泳操作，其可以将感兴趣的生物分子与游离探针分离，并且提供方便地收集感兴趣的所述生物分子。

公开(公告)号：US20140332382A1

公开(公告)日：2014-11-13

申请号：US14274409

申请日：2014-05-09

Applicant: The Regents of the University of California

Inventor： Amy E. Herr ,  Augusto Tentori

IPC: G01N27/447

CPC classification number: G01N27/44795 ,  G01N27/44773 ,  G01N27/44791

Abstract: Microfluidic devices and methods for using the same are provided. Embodiments include microfluidic devices that have a first separation region configured to separate a sample along a first directional axis based on a first property, and a second separation region in fluid communication with the first separation region and configured to separate the sample along a second directional axis based on a second property. Also provided are methods of using the devices as well as systems and kits that include the devices. The devices, systems and methods find use in a variety of different applications, including diagnostic and validation assays.

Abstract translation: 提供微流体装置及其使用方法。 实施例包括微流体装置，其具有被配置为基于第一属性沿着第一方向轴分离样品的第一分离区域和与第一分离区域流体连通的第二分离区域，并且被配置为沿着第二方向轴线分离样品 基于第二个属性。 还提供了使用设备的方法以及包括设备的系统和套件。 设备，系统和方法可用于各种不同的应用，包括诊断和验证测定。

公开(公告)号：US08580097B2

公开(公告)日：2013-11-12

申请号：US12709423

申请日：2010-02-19

Applicant: Tatsuo Kurosawa ,  Mitsuo Watanabe ,  Takuma Ohtsubo

Inventor： Tatsuo Kurosawa ,  Mitsuo Watanabe ,  Takuma Ohtsubo

IPC: G01N27/447 ,  G01N27/26

CPC classification number: G01N27/44773 ,  G01N27/44726

Abstract: Methods are provided for forming a complex comprising an analyte (or an analyte analogue) from a blood-derived sample and labeling substances, and separating the complex from excess labeling substances and coexisting substances from the blood-derived sample, in a rapid, simple, convenient, and highly precise isotachophoresis (ITP) process by adding 2-(N-morpholino)ethane sulfonate (MES) salt and/or glutamate salt to the ITP sample. Methods are also provided for measuring the analyte in blood-derived samples with high precision and high sensitivity, based on the amount of the complex separated or the amount of uncomplexed labeling substance-containing molecules.

Abstract translation: 提供了用于形成包含来自血液来源样品的分析物（或分析物类似物）和标记物质的复合物的复合物的方法，并且以快速，简单的方式将复合物与来自血液衍生的样品的过量标记物质和共存物质分离， 通过向ITP样品中加入2-（N-吗啉代）乙烷磺酸盐（MES）盐和/或谷氨酸盐，方便和高度精确的等速电泳（ITP）方法。 还提供了用于以高精度和高灵敏度测量血液来源的样品中的分析物的方法，基于分离的复合物的量或未复合的含标记物质的分子的量。