公开(公告)号：US20140272964A1

公开(公告)日：2014-09-18

申请号：US14207895

申请日：2014-03-13

Applicant: WiSys Technology Foundation, Inc.

Inventor： Wayne Schaefer ,  Ramaswamy Subramanian ,  Daniel Ferraro ,  Chi Li Yu ,  David Gibson ,  Swagatha Ghosh ,  P. Sai Sudha

IPC: G01N33/58 ,  C07K14/46

CPC classification number: G01N33/582 ,  C07K14/461 ,  G01N33/533

Abstract: This invention provides methods of making and using a fluorescent probe from the Sandercyanin protein as set forth in SEQ ID NO: 1 or SEQ ID NO: 2. In one embodiment, the invention provides a method of creating a fluorescent probe, comprising the steps of attaching a Sandercyanin moiety to a probe, wherein the probe is specific to a desired target.

Abstract translation: 本发明提供了制备和使用如SEQ ID NO：1或SEQ ID NO：2所示的来自桑an蛋白的荧光探针的方法。在一个实施方案中，本发明提供了一种产生荧光探针的方法，包括以下步骤： 将花青素部分附着到探针上，其中所述探针是特异于所需靶的。

公开(公告)号：US10962528B2

公开(公告)日：2021-03-30

申请号：US16065277

申请日：2016-12-20

Applicant: WISYS TECHNOLOGY FOUNDATION, INC.

Inventor： Ramaswamy Subramanian ,  Swagatha Ghosh ,  Wayne Ford Schaefer

IPC: C07K14/46 ,  C07K14/435 ,  C07K14/00 ,  G01N33/533 ,  G01N33/566 ,  G01N33/53

Abstract: Provided herein are monomeric variants of Sandercyanin fluorescent protein (SFP) including those monomeric SFP variants set forth in SEQ ID NO:2-6 and SFP variants with increased brightness. Also provided herein are methods of making and using fluorescent probes comprising such monomeric variants, where the fluorescent probes have specificity for desired targets.

公开(公告)号：US09383366B2

公开(公告)日：2016-07-05

申请号：US14207895

申请日：2014-03-13

Applicant: WiSys Technology Foundation, Inc.

Inventor： Wayne Schaefer ,  Ramaswamy Subramanian ,  Daniel Ferraro ,  Chi Li Yu ,  David Gibson ,  Swagatha Ghosh ,  P. Sai Sudha

IPC: G01N33/52 ,  G01N33/58 ,  C07K14/46 ,  G01N33/533

CPC classification number: G01N33/582 ,  C07K14/461 ,  G01N33/533

Abstract: This invention provides methods of making and using a fluorescent probe from the Sandercyanin protein as set forth in SEQ ID NO: 1 or SEQ ID NO: 2. In one embodiment, the invention provides a method of creating a fluorescent probe, comprising the steps of attaching a Sandercyanin moiety to a probe, wherein the probe is specific to a desired target.

Abstract translation: 本发明提供了制备和使用如SEQ ID NO：1或SEQ ID NO：2所示的来自桑an蛋白的荧光探针的方法。在一个实施方案中，本发明提供了一种产生荧光探针的方法，包括以下步骤： 将花青素部分附着到探针上，其中所述探针是特异于所需靶的。