公开(公告)号：US07881502B2

公开(公告)日：2011-02-01

申请号：US10852765

申请日：2004-05-21

申请人： William Carlson ,  Edwin Hirahara ,  Paul Roger Spencer

发明人： William Carlson ,  Edwin Hirahara ,  Paul Roger Spencer

IPC分类号： G06K9/00

CPC分类号： A01H1/04 ,  A01H4/00 ,  A01H4/006 ,  C12N5/04 ,  G02B21/0024

摘要： Methods and systems for three-dimensionally imaging an apical dome located at the cotyledon end of a plant embryo are provided. Three-dimensional information of an apical dome can be obtained by scanning the apical dome at varying focal planes along an axis of the embryo, or by using multiple cameras arranged in a confocal manner to image the apical dome. It can also be obtained by irradiating the apical dome with polychromatic light, wherein light beams of multiple wavelengths are focused at multiple focal planes along the axis of the embryo so that the dome's height information can be obtained based on the wavelength of the light precisely focused and reflected at each surface point of the dome. The three-dimensional information is used to ascertain various morphological features (e.g., size, shape, texture, etc.) of the apical dome, which are in turn used to assess the embryo's germinant vigor.

摘要翻译： 提供了用于三维地成像位于植物胚胎的子叶端的顶端圆顶的方法和系统。 可以通过沿着胚轴的不同焦平面扫描顶端圆顶，或者通过使用以共聚焦方式布置的多个相机来成像顶部圆顶来获得顶端圆顶的三维信息。 也可以通过用多色光照射顶端圆顶来获得，其中多个波长的光束沿着胚胎的轴线聚焦在多个焦平面处，使得可以基于精确聚焦的光的波长来获得圆顶的高度信息 并在圆顶的每个表面点处反射。 三维信息用于确定顶端圆顶的各种形态特征（例如，尺寸，形状，质地等），这再次用于评估胚胎的发芽活力。