公开(公告)号：US07667007B2

公开(公告)日：2010-02-23

申请号：US12102818

申请日：2008-04-14

申请人： William D. Huse ,  Herren Wu

发明人： William D. Huse ,  Herren Wu

IPC分类号： C07K16/28

CPC分类号： C07K16/2848 ,  A61K2039/505 ,  C07K2317/24 ,  C07K2317/565

摘要： The invention provides enhanced LM609 grafted antibodies exhibiting selective binding affinity to αvβ3, or a functional fragment thereof. The invention also provides nucleic acid molecules encoding the enhanced LM609 grafted antibodies. Additionally provided are methods of inhibiting a function of αvβ3 by contacting αvβ3 with an enhanced LM609 grafted antibody.

摘要翻译： 本发明提供增强的LM609接枝抗体，其表现出对αvβb或其功能片段的选择性结合亲和力。 本发明还提供编码增强的LM609接枝抗体的核酸分子。 另外提供了通过使αv＆bgr 3与增强的LM609接枝抗体接触来抑制αv和bgr 3的功能的方法。

公开(公告)号：US07175996B1

公开(公告)日：2007-02-13

申请号：US10110141

申请日：2000-10-13

申请人： Jeffry D. Watkins ,  William D. Huse ,  Herren Wu

发明人： Jeffry D. Watkins ,  William D. Huse ,  Herren Wu

IPC分类号： C12P21/06 ,  C12P21/08 ,  C07H21/04 ,  A61K39/395

CPC分类号： C07K16/465 ,  C07K16/00 ,  C07K16/2878 ,  C07K16/36 ,  C07K16/464 ,  C07K2317/24 ,  C07K2317/55 ,  C07K2317/56 ,  C07K2317/92

摘要： The present invention relates to compositions and methods of producing higher affinity molecules, and in particular, higher affinity antibodies. Specifically, the invention contemplates methods of mutagenesis to create a population of oligonucleotides coding for altered polypeptides that are expressed in a host cell and subsequently sequenced to determine the amino acid determinants conferring higher affinity.

摘要翻译： 本发明涉及产生更高亲和力分子的组合物和方法，特别是高亲和力抗体。 具体地，本发明考虑了诱变方法以产生编码在宿主细胞中表达的随后测序的改变的多肽的寡核苷酸群，以确定赋予更高亲和力的氨基酸决定簇。

公开(公告)号：US06849425B1

公开(公告)日：2005-02-01

申请号：US09434870

申请日：1999-11-04

申请人： William D. Huse ,  Jeffry D. Watkins ,  Herren Wu

发明人： William D. Huse ,  Jeffry D. Watkins ,  Herren Wu

IPC分类号： C07K16/28 ,  C07K16/36 ,  C07K16/46 ,  C12N15/00

CPC分类号： C07K16/465 ,  C07K16/00 ,  C07K16/2878 ,  C07K16/36 ,  C07K16/464 ,  C07K2317/24 ,  C07K2317/55 ,  C07K2317/56 ,  C07K2317/92

摘要： The invention provides a method of conferring donor CDR binding affinity onto an antibody acceptor variable region framework. The invention also provides a method of simultaneously grafting and optimizing the binding affinity of a variable region binding fragment. A method of optimizing the binding affinity of an antibody variable region is also provided.

摘要翻译： 本发明提供了将供体CDR结合亲和力赋予抗体受体可变区框架的方法。 本发明还提供了同时移植和优化可变区结合片段的结合亲和力的方法。 还提供了优化抗体可变区的结合亲和力的方法。

公开(公告)号：US07229619B1

公开(公告)日：2007-06-12

申请号：US09724531

申请日：2000-11-28

申请人： James F. Young ,  Scott Koenig ,  Leslie S. Johnson ,  William D. Huse ,  Jeffrey D. Watkins ,  Herren Wu

发明人： James F. Young ,  Scott Koenig ,  Leslie S. Johnson ,  William D. Huse ,  Jeffrey D. Watkins ,  Herren Wu

IPC分类号： A61K39/42

CPC分类号： C07K16/1027 ,  A61K2039/505 ,  C07K2317/24 ,  C07K2317/56 ,  C07K2317/565

摘要： The present invention encompasses novel antibodies and fragments thereof which immunospecifically bind to one or more RSV antigens and compositions comprising said antibodies and antibody fragments. The present invention encompasses methods preventing respiratory syncytial virus (RSV) infection in a human, comprising administering to said human a prophylactically effective amount of one or more antibodies or fragments thereof that immunospecifically bind to one or more RSV antigens, wherein a certain serum titer of said antibodies or antibody fragments is achieved in said human subject. The present invention also encompasses methods for treating or ameliorating symptoms associated with a RSV infection in a human, comprising administering to said human a therapeutically effective amount of one or more antibodies or fragments thereof that immunospecifically bind to one or more RSV antigens, wherein a certain serum titer of said antibodies or antibody fragments is achieved in said human subject. The present invention further encompasses compositions comprising antibodies or fragments thereof that immunospecifically bind to a RSV antigen, and methods using said compositions for detection or diagnosis a RSV infection.

摘要翻译： 本发明包括免疫特异性结合一种或多种RSV抗原的新型抗体及其片段，以及包含所述抗体和抗体片段的组合物。 本发明包括预防人类呼吸道合胞病毒（RSV）感染的方法，包括向所述人施用预防有效量的一种或多种免疫特异性结合一种或多种RSV抗原的抗体或其片段，其中一定的血清滴度为 所述抗体或抗体片段在所述人受试者中实现。 本发明还包括用于治疗或改善与人类RSV感染有关的症状的方法，包括向所述人施用治疗有效量的一种或多种免疫特异性结合一种或多种RSV抗原的抗体或其片段，其中一定 所述抗体或抗体片段的血清滴度在所述人受试者中实现。 本发明还包括包含免疫特异性结合RSV抗原的抗体或其片段的组合物，以及使用所述组合物检测或诊断RSV感染的方法。

公开(公告)号：US07179900B2

公开(公告)日：2007-02-20

申请号：US10403180

申请日：2003-03-31

申请人： James F. Young ,  Scott Koenig ,  Leslie S. Johnson ,  William D. Huse ,  Jeffrey D. Watkins ,  Herren Wu

发明人： James F. Young ,  Scott Koenig ,  Leslie S. Johnson ,  William D. Huse ,  Jeffrey D. Watkins ,  Herren Wu

IPC分类号： A61K39/42 ,  A61K39/155

CPC分类号： C07K16/1027 ,  A61K2039/505 ,  C07K2317/24 ,  C07K2317/56 ,  C07K2317/565

摘要： The present invention encompasses novel antibodies and fragments thereof which immunospecifically bind to one or more RSV antigens and compositions comprising said antibodies and antibody fragments. The present invention encompasses methods preventing respiratory syncytial virus (RSV) infection in a human, comprising administering to said human a prophylactically effective amount of one or more antibodies or fragments thereof that immunospecifically bind to one or more RSV antigens, wherein a certain serum titer of said antibodies or antibody fragments is achieved in said human subject. The present invention also encompasses methods for treating or ameliorating symptoms associated with a RSV infection in a human, comprising administering to said human a therapeutically effective amount of one or more antibodies or fragments thereof that immunospecifically bind to one or more RSV antigens, wherein a certain serum titer of said antibodies or antibody fragments is achieved in said human subject. The present invention further encompasses compositions comprising antibodies or fragments thereof that immunospecifically bind to a RSV antigen, and methods using said compositions for detection or diagnosis of a RSV infection.

摘要翻译： 本发明包括免疫特异性结合一种或多种RSV抗原的新型抗体及其片段，以及包含所述抗体和抗体片段的组合物。 本发明包括预防人类呼吸道合胞病毒（RSV）感染的方法，包括向所述人施用预防有效量的一种或多种免疫特异性结合一种或多种RSV抗原的抗体或其片段，其中一定的血清滴度为 所述抗体或抗体片段在所述人受试者中实现。 本发明还包括用于治疗或改善与人类RSV感染有关的症状的方法，包括向所述人施用治疗有效量的一种或多种免疫特异性结合一种或多种RSV抗原的抗体或其片段，其中一定 所述抗体或抗体片段的血清滴度在所述人受试者中实现。 本发明还包括包含免疫特异性结合RSV抗原的抗体或其片段的组合物，以及使用所述组合物检测或诊断RSV感染的方法。

公开(公告)号：US08153133B2

公开(公告)日：2012-04-10

申请号：US12707527

申请日：2010-02-17

申请人： James F. Young ,  Leslie S. Johnson ,  William D. Huse ,  Herren Wu ,  Jeffry D. Watkins

发明人： James F. Young ,  Leslie S. Johnson ,  William D. Huse ,  Herren Wu ,  Jeffry D. Watkins

IPC分类号： A61K39/42

CPC分类号： C07K16/1027 ,  A61K2039/505 ,  C07K16/00 ,  C07K2317/567

摘要： High potency antibodies, including immunologically active fragments thereof, having high kinetic association rate constants and optional high affinities are disclosed, along with methods for producing such antibodies. The high potency antibodies disclosed herein are of either the neutralizing or non-neutralizing type and have specificity for antigens displayed by microorganisms, especially viruses, as well as antigenic sites present on cancer cells and on various types of toxins, and the products of toxins. Processes for producing high potency neutralizing antibodies and increasing the potency of already existing neutralizing antibodies are also described. Methods of using said antibodies in the prevention and/or treatment of diseases, especially diseases induced or caused by viruses, are disclosed.

摘要翻译： 公开了具有高动力学关联速率常数和任选的高亲和力的高效力抗体，包括其免疫活性片段，以及产生此类抗体的方法。 本文公开的高效力抗体是中和或非中和型，并且对由微生物，特别是病毒以及存在于癌细胞和各种类型毒素上的抗原位点以及毒素产物显示的抗原具有特异性。 还描述了用于产生高效中和抗体并增加已经存在的中和抗体的效力的方法。 公开了在预防和/或治疗疾病，特别是由病毒引起或引起的疾病中使用所述抗体的方法。

公开(公告)号：US07847082B2

公开(公告)日：2010-12-07

申请号：US12476183

申请日：2009-06-01

申请人： James F. Young ,  Scott Koenig ,  Leslie S. Johnson ,  William D. Huse ,  Jeffrey D. Watkins ,  Herren Wu

发明人： James F. Young ,  Scott Koenig ,  Leslie S. Johnson ,  William D. Huse ,  Jeffrey D. Watkins ,  Herren Wu

IPC分类号： C07H21/04 ,  A61K39/42 ,  A61K39/155

CPC分类号： C07K16/1027 ,  A61K2039/505 ,  C07K2317/24 ,  C07K2317/56 ,  C07K2317/565

摘要： The present invention encompasses novel antibodies and fragments thereof which immunospecifically bind to one or more RSV antigens and compositions comprising said antibodies and antibody fragments. The present invention encompasses methods preventing respiratory syncytial virus (RSV) infection in a human, comprising administering to said human a prophylactically effective amount of one or more antibodies or fragments thereof that immunospecifically bind to one or more RSV antigens, wherein a certain serum titer of said antibodies or antibody fragments is achieved in said human subject. The present invention also encompasses methods for treating or ameliorating symptoms associated with a RSV infection in a human, comprising administering to said human a therapeutically effective amount of one or more antibodies or fragments thereof that immunospecifically bind to one or more RSV antigens, wherein a certain serum titer of said antibodies or antibody fragments is achieved in said human subject. The present invention further encompasses compositions comprising antibodies or fragments thereof that immunospecifically bind to a RSV antigen, and methods using said compositions for detection or diagnosis a RSV infection.

摘要翻译： 本发明包括免疫特异性结合一种或多种RSV抗原的新型抗体及其片段，以及包含所述抗体和抗体片段的组合物。 本发明包括预防人类呼吸道合胞病毒（RSV）感染的方法，包括向所述人施用预防有效量的一种或多种免疫特异性结合一种或多种RSV抗原的抗体或其片段，其中一定的血清滴度为 所述抗体或抗体片段在所述人受试者中实现。 本发明还包括用于治疗或改善与人类RSV感染有关的症状的方法，包括向所述人施用治疗有效量的一种或多种免疫特异性结合一种或多种RSV抗原的抗体或其片段，其中一定 所述抗体或抗体片段的血清滴度在所述人受试者中实现。 本发明还包括包含免疫特异性结合RSV抗原的抗体或其片段的组合物，以及使用所述组合物检测或诊断RSV感染的方法。

公开(公告)号：US20100266614A1

公开(公告)日：2010-10-21

申请号：US12777814

申请日：2010-05-11

申请人： James F. YOUNG ,  Leslie S. Johnson ,  William D. Huse ,  Herren Wu ,  Jeffry D. Watkins

发明人： James F. YOUNG ,  Leslie S. Johnson ,  William D. Huse ,  Herren Wu ,  Jeffry D. Watkins

IPC分类号： A61K39/42 ,  C07K16/08 ,  A61P31/12

CPC分类号： C07K16/00 ,  A61K2039/505 ,  C07K16/1027 ,  C07K2317/24 ,  C07K2317/565 ,  C07K2317/76 ,  C07K2317/92 ,  C07K2319/00

摘要： Ultra high affinity antibodies with binding affinities in the range of 1010 M−1, and even 1011 M−1 are disclosed. Such antibodies include antibodies having novel high affinity complementarity determining regions (CDRs), especially those with framework and constant regions derived from either humans or mice. Methods of preparing and screening such antibodies, as well as methods of using them to prevent and/or treat disease, especially virus-induced diseases, are also disclosed.

摘要翻译： 公开了具有1010M-1甚至1011M-1范围内的结合亲和力的超高亲和力抗体。 此类抗体包括具有新型高亲和力互补决定区（CDR）的抗体，特别是具有来自人或小鼠的骨架和恒定区的抗体。 还公开了制备和筛选这些抗体的方法，以及使用它们来预防和/或治疗疾病，特别是病毒诱导的疾病的方法。

公开(公告)号：US07323172B2

公开(公告)日：2008-01-29

申请号：US10962285

申请日：2004-10-08

申请人： James F. Young ,  Scott Koenig ,  Leslie S. Johnson ,  William D. Huse ,  Jeffrey D. Watkins ,  Herren Wu

发明人： James F. Young ,  Scott Koenig ,  Leslie S. Johnson ,  William D. Huse ,  Jeffrey D. Watkins ,  Herren Wu

IPC分类号： A61K39/42 ,  A61K39/155

CPC分类号： C07K16/1027 ,  A61K2039/505 ,  C07K2317/24 ,  C07K2317/56 ,  C07K2317/565

摘要： The present invention encompasses novel antibodies and fragments thereof which immunospecifically bind to one or more RSV antigens and compositions comprising said antibodies and antibody fragments. The present invention encompasses methods preventing respiratory syncytial virus (RSV) infection in a human, comprising administering to said human a prophylactically effective amount of one or more antibodies or fragments thereof that immunospecifically bind to one or more RSV antigens, wherein a certain serum titer of said antibodies or antibody fragments is achieved in said human subject. The present invention also encompasses methods for treating or ameliorating symptoms associated with a RSV infection in a human, comprising administering to said human a therapeutically effective amount of one or more antibodies or fragments thereof that immunospecifically bind to one or more RSV antigens, wherein a certain serum titer of said antibodies or antibody fragments is achieved in said human subject. The present invention further encompasses compositions comprising antibodies or fragments thereof that immunospecifically bind to a RSV antigen, and methods using said compositions for detection or diagnosis a RSV infection.

摘要翻译： 本发明包括免疫特异性结合一种或多种RSV抗原的新型抗体及其片段，以及包含所述抗体和抗体片段的组合物。 本发明包括预防人类呼吸道合胞病毒（RSV）感染的方法，包括向所述人施用预防有效量的一种或多种免疫特异性结合一种或多种RSV抗原的抗体或其片段，其中一定的血清滴度为 所述抗体或抗体片段在所述人受试者中实现。 本发明还包括用于治疗或改善与人类RSV感染有关的症状的方法，包括向所述人施用治疗有效量的一种或多种免疫特异性结合一种或多种RSV抗原的抗体或其片段，其中一定 所述抗体或抗体片段的血清滴度在所述人受试者中实现。 本发明还包括包含免疫特异性结合RSV抗原的抗体或其片段的组合物，以及使用所述组合物检测或诊断RSV感染的方法。

公开(公告)号：US06656467B2

公开(公告)日：2003-12-02

申请号：US09771415

申请日：2001-01-26

申请人： James F. Young ,  Leslie S. Johnson ,  William D. Huse ,  Herren Wu ,  Jeffry D. Watkins

发明人： James F. Young ,  Leslie S. Johnson ,  William D. Huse ,  Herren Wu ,  Jeffry D. Watkins

IPC分类号： A61K3942

CPC分类号： C07K16/00 ,  A61K2039/505 ,  C07K16/1027 ,  C07K2317/24 ,  C07K2317/565 ,  C07K2317/76 ,  C07K2317/92 ,  C07K2319/00

摘要： Ultra high affinity antibodies with binding affinities in the range of 1010 M−1, and even 1011 M−1 are disclosed. Such antibodies include antibodies having novel high affinity complementarity determining regions (CDRs), especially those with framework and constant regions derived from either humans or mice. Methods of preparing and screening such antibodies, as well as methods of using them to prevent and/or treat disease, especially virus-induced diseases, are also disclosed.

摘要翻译： 公开了具有10 10 M -1，甚至10 11 M -1范围内的结合亲和力的超高亲和力抗体。 此类抗体包括具有新型高亲和力互补决定区（CDR）的抗体，特别是具有来自人或小鼠的骨架和恒定区的抗体。 还公开了制备和筛选这些抗体的方法，以及使用它们来预防和/或治疗疾病，特别是病毒诱导的疾病的方法。